Razi Journal of Medical Sciences
مجله علوم پزشکی رازی
RJMS
Medical Sciences
http://rjms.iums.ac.ir
39
journal39
2228-7043
2228-7051
en
jalali
1402
1
1
gregorian
2023
4
1
30
2
online
1
fulltext
fa
مطالعه اثر آنتی اکسیدانی و ضد التهابی عصاره هیدرواتانولی گل گیاه همیشه بهار بر بافت مغز استخوان در موشهای صحرایی نر درمان شده با سیکلوفسفامید
Study of Antioxidant and Anti-inflammatory Effects of Calendula officinalis L. Hydrethanolic Extract on Bone Marrow Tissue in Wistar Rats Treated with Cyclophosphamide
فیزیولوژی
Physiology
پژوهشي
Research
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="tab-stops:10.5pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:115%"><span style="font-family:Calibri,sans-serif"><b><span lang="FA" style="font-size:9.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:#0070c0">زمینه و هدف: </span></span></span></span></b><span lang="FA" style="font-size:9.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black">مصرف داروهای سایتوتوکسیک مانند سیکلوفسفامید در روند شیمی درمانی برخی از سرطانها رایج میباشد. عوارض جانبی این گونه داروها بسیار مهم میباشند. گیاهان دارویی به دلیل خواص آنتی اکسیدانی به عنوان داروهای درمانی اصلی یا مکمل، میتوانند از عوارض جانبی داروهای شیمیایی بکاهند. هدف از این مطالعه تجربی بررسی اثرات آنتی اکسیدانی و ضد التهابی عصاره گل گیاه همیشه بهار بر بافت مغز استخوان در موشهای صحرایی نر درمان شده با سیکلوفسفامید میباشد.</span></span></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="tab-stops:10.5pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:115%"><span style="font-family:Calibri,sans-serif"><b><span lang="FA" style="font-size:9.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:#0070c0">روش کار:</span></span></span></span></b><span lang="FA" style="font-size:9.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black"> در این مطالعه تجربی، 35 سر موش صحرائی نر نژاد ویستار در محدوده وزنی 250-220 گرم مورد استفاده قرار گرفت. موشها در 5 گروه 7 سری به طور تصادفی تقسیم شدند: کنترل (روزانه 5/0 میلی لیتر نرمال سالین)، گروه شاهد (سیکلوفسفامید، 15 میلی گرم بر کیلو گرم وزن بدن، روزانه)، گروه کنترل مثبت (روزانه 600 میلی گرم بر کیلوگرم وزن بدن عصاره گل گیاه همیشه بهار)، تیمار 1 (سیکلوفسفامید، 15 میلی گرم بر کیلو گرم وزن بدن + عصاره گل گیاه همیشه بهار 300 میلی گرم بر کیلوگرم وزن بدن، روزانه) و تیمار2 (سیکلوفسفامید، 15 میلی گرم بر کیلو گرم وزن بدن + عصاره گل گیاه همیشه بهار 600 میلی گرم بر کیلوگرم وزن بدن، روزانه). سیکلوفسفامید و عصاره به مدت 15 روز تجویز شدند. تمامی تزریقات درون صفاقی انجام شد. در پایان آزمایش، حیوانات توسط کتامین هیدروکلراید + زایلازین بیهوش شده و خون گیری مستقیم از قلب جهت بررسی پارامترهای سرمی نظیر </span></span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:black">NO</span></span></span></span><span lang="FA" style="font-size:9.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black"> ، </span></span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:black">GSH</span></span></span></span><span lang="FA" style="font-size:9.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black"> ، </span></span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:black">MDA</span></span></span></span><span lang="FA" style="font-size:9.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black"> ، </span></span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:black">GPx</span></span></span></span><span lang="FA" style="font-size:9.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black"> ، </span></span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:black">TNF-α</span></span></span></span><span lang="FA" style="font-size:9.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black"> و </span></span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:black">IL-6β</span></span></span></span><span lang="FA" style="font-size:9.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black"> انجام شد. استخوان جناغ موشها جهت مطالعات بافت شناسی جدا و توسط فرمالین10% فیکس و مقاطع بافتی آنها تهیه و مطالعه شد. دادههای به دست آمده با استفاده از آنالیز واریانس یک طرفه و تست توکی ارزیابی شدند. </span></span></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="tab-stops:10.5pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:115%"><span style="font-family:Calibri,sans-serif"><b><span lang="FA" style="font-size:9.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:#0070c0">یافتهها: </span></span></span></span></b><span lang="FA" style="font-size:9.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black">نتایج این مطالعه نشان داد که سیکلوفسفامید موجب افزایش سطح سرمی پارامترهای </span></span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:black">NO</span></span></span></span><span lang="FA" style="font-size:9.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black"> ، </span></span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:black">MDA</span></span></span></span><span lang="FA" style="font-size:9.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black"> ، </span></span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:black">TNF-α</span></span></span></span><span lang="FA" style="font-size:9.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black"> و </span></span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:black">IL-6β</span></span></span></span><span lang="FA" style="font-size:9.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black"> و کاهش سطح سرمی پارامترهای </span></span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:black">GSH</span></span></span></span><span lang="FA" style="font-size:9.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black"> و </span></span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:black">GPx</span></span></span></span><span lang="FA" style="font-size:9.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black"> گردید. علاوه بر این، سیکلوفسفامید اثر تخریبی بر بافت خون ساز مغز استخوان داشت. عصاره گل گیاه همیشه بهار توانست از تغییرات ایجاد شده ی پارامترهای خونی و تخریب بافت مغز استخوان توسط سیکلوفسفامید جلوگیری نماید. </span></span></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="tab-stops:10.5pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:115%"><span style="font-family:Calibri,sans-serif"><b><span lang="FA" style="font-size:9.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:#0070c0">نتیجهگیری:</span></span></span></span></b><span lang="FA" style="font-size:9.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black"> عصارهی هیدرواتانولی گل گیاه همیشه بهار دارای اثرات آنتی اکسیدانی و ضد التهابی بر بافت خون ساز مغز استخوان میباشد.</span></span></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="tab-stops:10.5pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:115%"><span style="font-family:Calibri,sans-serif"><span lang="AR-SA" style="font-size:9.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black"></span></span></span></span></span></span></span></span></span></span></span></span><br>
<div style="text-align: justify;"><span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="tab-stops:10.5pt"><span style="line-height:115%"><span style="font-family:Calibri,sans-serif"><span style="font-size:8.5pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:black"></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="tab-stops:10.5pt"><span style="line-height:115%"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:8.5pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:#0070c0">Background & Aims:</span></span></span></span></b> <span lang="EN" style="font-size:8.5pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:black">Some drugs used in chemotherapy have destructive effects on hematopoietic tissue.</span></span></span></span><span style="font-size:8.5pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:black">Cyclophosphamide is a commonly used anti-cancer drug which used for some of malignant cancers treatment and causes toxicity by its reactive metabolites such as acrolein and phosphoramide mustard. This drug has oxidant, inflammatory and subversion effects on blood hemopoetic bone marrow tissue.</span></span></span></span> <span style="font-size:8.5pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:black">Medicinal plants with antioxidant and anti-inflammatory effects have protective effects on bone marrow tissue.</span></span></span></span> <span style="font-size:8.5pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:black">The aim of this experimental study is to investigate antioxidant and anti-infammatory effects of <i>Calendula officinalis</i> L. hydroethanolic extract (CHE) on bone marrow in Wistar rats treated with cyclophosphamide.</span></span></span></span><b> </b><span dir="RTL" lang="FA" style="font-size:8.5pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black"></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="tab-stops:10.5pt"><span style="line-height:115%"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:8.5pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:#0070c0">Methods:</span></span></span></span></b> <span style="font-size:8.5pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:black">In this experimental study 35 male Wistar rats (220-250 g) were divided in 5 groups (n=7): control (Normal saline, 0.5 ml), Cyclophosphamide (CP, 15mg/kg), positive control (CHE, 600 mg/kg), treatment 1 (CP, 15mg/kg + CHE, 300 mg/kg) and treatment 2 (CP, 15mg/kg + CHE, 600 mg/kg). All injections were done interaperituneally and the experiments were done for 15 days. At the end of experiments all animals were anesthetized ( ketamine hydrochloride, 50mg/kg + xylasine hydrochloride, 10mg/kg). </span></span></span></span><span lang="EN" style="font-size:8.5pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:black">After splitting the chest with surgical scissor, blood samples were collected directly from the right ventricle of the heart</span></span></span></span><span style="font-size:8.5pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:black">. </span></span></span></span><span lang="EN" style="font-size:8.5pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:black">The collected blood samples were centrifuged at 4000 rpm for 5 minutes and their serums were separated</span></span></span></span><span style="font-size:8.5pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:black">.</span></span></span></span><span lang="EN" style="font-size:8.5pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:black"> Serums prepared send to the laboratory for measuring serum parameters</span></span></span></span><span style="font-size:8.5pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:black"> such as: Nitric oxide(NO), Glutamine (GSH), Malondialdehyde (MDA), Glutathione peroxidase (GPx), Tumor Necrosis Factor- alfa(TNF-α) and Inetrlukin-6β (IN-6β).</span></span></span></span><span lang="EN" style="font-size:8.5pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:black"> In addition, rat sternum's tissue samples were isolated and fixed with 10% </span></span></span></span><span style="font-size:8.5pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:black">Formaldehyde</span></span></span></span><span lang="EN" style="font-size:8.5pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:black">. Bone marrow tissue samples were studied in the histology laboratory after various steps and preparation of microscopic sections. Data were analyzed using one-way analysis of variance and Tukey's test. The significant difference of the data were considered with <i>p</i> <0.05.</span></span></span></span> <span style="font-size:8.5pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:black"></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="tab-stops:10.5pt"><span style="line-height:115%"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:8.5pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:#0070c0">Results</span></span></span></span></b><span style="font-size:8.5pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:#0070c0">: </span></span></span></span><span lang="EN" style="font-size:8.5pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:black">The results of this study showed that cyclophosphamide caused a significant increase in serum TNF-α levels in the group receiving cyclophosphamide compared to the control group (<i>p</i><0.001).</span></span></span></span> <span lang="EN" style="font-size:8.5pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:black">While in the positive control group and treatments 1 and 2 groups, there was no significant difference compared to the control group. Also, there was no significant difference between treatment 1 and treatment 2 groups. Cyclophosphamide significantly increased serum IL-6β levels in the CP group compared to the control group (<i>p</i><0.001). However, serum levels of IL-6β in the groups treated with CHE were significantly reduced compared to the CP group. There was significant difference of serum levels of IL-6β between treatment 1 and treatment 2 groups (<i>p</i><0. 01). The use of cyclophosphamide showed that the NO serum level in the CP group was significantly higher than the control group. The groups treated with CHE had a significant decrease in NO serum level compared to the CP group (<i>p</i><0.001). There was significant difference of serum levels of NO serum level between treatment 1 and treatment 2 groups (<i>p</i><0. 001). In addition, MDA serum levels in the CP group were significantly increased compared to the control group. There was significant difference of serum levels of MDA serum levels between treatment 1 and treatment 2 groups (<i>p</i><0. 001). There was significant differences of MDA serum levels in CP group, positive group and treatment groups 1&2 (<i>p</i><0. 001). Another result of this study was that cyclophosphamide significantly reduced serum levels of GSH and GPx in the CP group compared with control group (<i>p</i><0. 001). While serum levels of these parameters in positive control groups and treatments 1 and 2 showed a significant increase compared to the CP group. There were no significant differences of GPx and GSH serum levels between treatment groups 1&2. Histological results in this study showed that CP reduces cell density, loss of cell cohesion and also infiltration of lymphocytes in bone marrow tissue samples in CP group. These results also showed that in treatment group 1 cell density is relatively higher than CP group. In treatment group 2, cell density increased and a significant decrease in inflammatory cells were observed. No significant changes were observed in the positive control group compared to the control group.</span></span></span></span><span style="font-size:8.5pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:black"></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="tab-stops:10.5pt"><span style="line-height:115%"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:8.5pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:#0070c0">Conclusion</span></span></span></span></b><span style="font-size:8.5pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:#0070c0">: </span></span></span></span><span lang="EN" style="font-size:8.5pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:black">The results of this study showed that cyclophosphamide has destructive effects on various tissues of the body, including hematopoietic tissue of bone marrow in test rats. The effects of oxidative stress and cell death of cyclophosphamide have been previously reported. The use of drugs used in the process of chemotherapy is very common and inevitable. Cyclophosphamide, which is used in pediatric oncology as part of standard treatment for leukemia, solid tumors and brain tumors, is one of these drugs. Cyclophosphamide, as a cytotoxic drug, causes inflammation and increases inflammatory cytokines such as tumor necrosis factor-alpha (TNF-α), IL-1β and IL-6β.</span></span></span></span> <span lang="EN" style="font-size:8.5pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:black">Cyclophosphamide has been identified as one of the most important tissue toxicity agents due to its reactive metabolites such as acrolein and phosphoramide mustard and it has been shown that this anticancer drug increases inflammatory serum factors such as TNF-α and nitric oxide (NO) and decreases regenerative glutathione (GSH). In various studies, the antioxidant effects of CHE extract have been proven. Many chemical compounds have been identified in CHE, including carotenoids, flavonoids, terpenoids, coumarins, quinones, amino acids, lipids, and carbohydrates. These compounds can be used for various medicinal and therapeutic purposes as an antioxidant, anti-inflammatory, antibacterial, antifungal, anti-cancer, anti-HIV, for wound healing and more. The main chemical constituents of CHE include steroids, terpenoids, free and esterified terterpene alcohols, phenolic acids, flavonoids (quercetin, rutin, narcissin, isoramentin, campferrol) and other compounds. Research has shown that coumarins have very beneficial effects on cell proliferation and preventing apoptosis. Large amounts of coumarin have been mentioned in the extract of </span></span></span></span><i><span style="font-size:8.5pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:black">Calendula officinalis</span></span></span></span></i><i><span lang="EN" style="font-size:8.5pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:black">'s</span></span></span></span></i><span lang="EN" style="font-size:8.5pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:black"> extract. Coumarins have been used to treat prostate cancer, renal cell carcinoma and leukemia. In addition, coumarins play an important role in preventing side effects from radiotherapy.</span></span></span></span> <span lang="EN" style="font-size:8.5pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:black">The antioxidant activity of CHE was shown in a study performed on polymorphonuclear leukocytes.</span></span></span></span> <span lang="EN" style="font-size:8.5pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:black">Flavonoids and phenolic compounds with iron-binding region have iron chelating properties. By flaking iron, flavonoids both absorb more iron from the intestines and prevent it from being excreted by the kidneys. By doing this, flavonoids store iron in tissues, especially in hematopoietic tissues. </span></span></span></span><i><span style="font-size:8.5pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:black">Calendula officinalis</span></span></span></span></i><i><span lang="EN" style="font-size:8.5pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:black">'s</span></span></span></span></i><span lang="EN" style="font-size:8.5pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:black"> flowers contain large amounts of antioxidant compounds (flavonoids and polyphenols), which indicates the antioxidant properties of this plant. The presence of propylene glycol in CHE has been effective in preventing the formation of oxidizing free radicals such as ROS and RNS in isolated leukocytes in the respiratory tract. The protective effects of CHE on inflammatory parameters and serum antioxidants seem to be related to its antioxidant and anti-inflammatory effects. Therefore, it is thought that CHE extract, due to its antioxidant and anti-inflammatory agents, is able to prevent the destructive effects of cyclophosphamide on hematopoietic tissue.</span></span></span></span><span style="font-size:8.5pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:black"></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="tab-stops:10.5pt"><span style="line-height:115%"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:8.5pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:#0070c0"></span></span></span></span></b></span></span></span></span></span></span></div>
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<span style="font-size:11pt"><span style="background:#f8f9fa"><span style="unicode-bidi:embed"><span style="line-height:115%"><span sans-serif="" style="font-family:Calibri,"><span new="" roman="" style="font-family:" times=""><span style="color:#202124"></span></span></span></span></span></span></span>
گل همیشه بهار, نشانگرهای اکسیدانی, مغز استخوان, سیکلو فسفامید
Calendula officinalis, Ant-ioxidant markers, Bone marrow, Cyclophosphamide
25
39
http://rjms.iums.ac.ir/browse.php?a_code=A-10-3553-4&slc_lang=fa&sid=1
Ida
Shahabi Baher
آیدا
شهابی باهر
3900319475328460074233
3900319475328460074233
No
MSc in Animal Physiology, Department of Biology, Faculty of Basic Sciences, Bu-Ali Sina University, Hamedan, Iran
کارشناس ارشد، فیزیولوژی جانوری، گروه زیست شناسی، دانشکده علوم پایه، دانشگاه بوعلی سینا، همدان، ایران
Naser
Mirazi
ناصر
میرازی
mirazi205@gmail.com
3900319475328460074234
3900319475328460074234
Yes
Professor, Department of Biology, Faculty of Basic Sciences, Bu-Ali Sina University, Hamedan, Iran
استاد، گروه زیست شناسی، دانشکده علوم پایه، دانشگاه بوعلی سینا، همدان
Zahra
Izadi
زهرا
ایزدی
3900319475328460074235
3900319475328460074235
No
Assistant Professor, Department of Horticulture, Nahavand Higher Education Complex, Bu-Ali Sina University, Hamedan, Iran
استادیار، گروه علوم و مهندسی باغبانی، مجتمع آموزش عالی نهاوند، دانشگاه بوعلی سینا، همدان، ایران
Alireza
Nourian
علیرضا
نوریان
3900319475328460074236
3900319475328460074236
No
Associate Professor, Department of Pathobiology, Faculty of Veterinary Medicine, Bu-Ali Sina University, Hamedan, Iran
دانشیار، گروه پاتوبیولوژی، دانشکده پیرادامپزشکی، دانشگاه بوعلی سینا، همدان، ایران
Samaneh
Safari
سمانه
صفری
3900319475328460074237
3900319475328460074237
No
PhD Student, Department of Neuroscience, School of Science and Advanced Technologies in Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
دانشجوی دکتری، گروه علوم اعصاب، دانشکده علوم و فناوری ها ی پیشرفته در پزشکی، دانشگاه علوم پزشکی همدان، همدان، ایران