Razi Journal of Medical Sciences
مجله علوم پزشکی رازی
RJMS
Medical Sciences
http://rjms.iums.ac.ir
39
journal39
2228-7043
2228-7051
en
jalali
1401
12
1
gregorian
2023
3
1
29
12
online
1
fulltext
fa
اثر همزمان تمرین مقاومتی با شدت بالا همراه با مکمل ارگانیک و غیرارگانیک بر بیان ژن myoD در عضله نعلی موشهای نر بالغ
Simultaneous Effect of a High-Intensity Resistance Training with Organic and Inorganic Supplements on myoD Gene Expression in the Horseshoe Muscle of Adult Male Mice
فیزیولوژی ورزش
Exercise Physiology
پژوهشي
Research
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="tab-stops:10.5pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:115%"><span style="font-family:Calibri,sans-serif"><b><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:#0070c0">زمینه و هدف: </span></span></span></span></b><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black">مصرف مکمل­ها راهکاری مناسب در جهت مهار عوارض ناشی از فعالیت­های بدنی است، بنابراین هدف مطالعه، بررسی اثر</span></span></span></span><b> </b><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black">همزمان یک جلسه تمرین</span></span></span></span><b> </b><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black">مقاومتی</span></span></span></span><b> </b><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black">با شدت بالا</span></span></span></span><b> </b><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black">و</span></span></span></span><b> </b><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black">مقایسه عملکرد</span></span></span></span><b> </b><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black">مکملهای</span></span></span></span><b> </b><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black">ارگانیک</span></span></span></span><b> </b><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black">و</span></span></span></span><b> </b><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black">غیر</span></span></span></span><b> </b><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black">ارگانیک بر</span></span></span></span><b> </b><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black">بیان</span></span></span></span><b> </b><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black">ژن</span></span></span></span><b> </b><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:black">myoD</span></span></span></span><span style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black"> در</span></span></span></span><b> </b><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black">عضله کند انقباض عضله نعلی</span></span></span></span><b> </b><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black">موشهای نر</span></span></span></span><b> </b><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black">بالغ</span></span></span></span><b> </b><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black">انجام شد</span></span></span></span><span lang="X-NONE" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black">.</span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black"></span></span></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="tab-stops:10.5pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:115%"><span style="font-family:Calibri,sans-serif"><b><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:#0070c0">روش کار</span></span></span></span></b><b><span lang="X-NONE" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:#0070c0">:</span></span></span></span></b><b> </b><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black">در این مطالعه آزمایشی، تعداد 40</span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black"> موش نژاد ویستار با میانگین </span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black">وزن 200- 100 گرم تهیه و در گروههای </span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black">کنترل (10 = تعداد)</span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black">، </span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black">تمرین (10 = تعداد)، اسپیرولینا+ تمرین (10 = تعداد)، گلوتامین+</span></span></span></span><b> </b><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black">تمرین (10 = تعداد) تقسیم شدند. موشهای گروه تمرین، مدت دو هفته برنامه تمرین 3 روز در هر هفته راه رفتن روی سطح شیبدار (</span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black">۴ </span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black">ست، </span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black">۵ </span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black">تکرار، </span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black">۳۰ </span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black">ثانیه استراحت بین تکرارها) انجام دادند و گروه مکمل+ تمرین، برنامه مصرف مکمل پنج روز قبل از اجرای پروتکل اصلی روزانه یک بار نیم گرم /کیلوگرم وزن بدن مصرف داشتند. </span></span></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="tab-stops:10.5pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:115%"><span style="font-family:Calibri,sans-serif"><b><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:#0070c0">یافتهها:</span></span></span></span></b><b> </b><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black">نتایج تحلیل واریانس نشان داد که بین</span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black"> میزان بیان نسبی ژن </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:black">MyoD</span></span></span></span><span style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black"> در گروههای مورد مطالعه تفاوت معنیداری وجود دارد [001/0 </span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:black">≥</span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black"> P و 182/1010= F] و همچنین نتایج آزمون تعقیبی بونفرونی نشان داد که این تفاوت بین گروه­ تمرین و تمرین+ </span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black">اسپیرولینا</span></span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black">(001/0 </span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:black">≥</span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black"> P) و نیز گروه تمرین و </span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black">تمرین+ گلوتامین </span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black">معنیدار میباشد (001/0 </span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:black">≥</span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black"> P). در حالی که بین دو گروه تمرین+ </span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black">اسپیرولینا و تمرین+ گلوتامین تفاوت معنیداری مشاهده نشد </span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black">(245/0 = P). </span></span></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="tab-stops:10.5pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:115%"><span style="font-family:Calibri,sans-serif"><b><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:#0070c0">نتیجهگیری</span></span></span></span></b><b><span lang="X-NONE" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:#0070c0">:</span></span></span></span></b> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black">به طور کلی میتوان نتیجه گیری کرد که مصرف مکمل ارگانیک، شیوهی مناسبی جهت جلوگیری از کاهش بیان و میزان آسیبهای وارده به تارهای عضلانی </span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black">پس از ورزش مقاومت با شدت بالا </span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black">است</span></span></span></span><span lang="X-NONE" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""><span style="color:black">.</span></span></span></span></span></span></span></span></span></span></span></span>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="tab-stops:10.5pt"><span style="line-height:115%"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:9.5pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:#0070c0">Background & Aims:</span></span></span></span></b> <span style="font-size:9.5pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:black">Increasing the intensity of physical activity and as a result increasing oxidative stress causes free radicals in the body and these free radicals destroy cell biological structures such as proteins, fats, membranes, and structures. They are inherited. Considering that taking supplements can be a good way to control the effects of strenuous physical activity; therefore, it is important to study the mechanism of action of complementary compounds of organic and non-organic origin and compare their effects and performance at the biochemical and genetic level. In this regard, the study of the expression of genes related to the inhibition of oxidative stress can be a direct and appropriate solution to evaluate the function of various supplements. The satellite cells beneath the skeletal muscle basement membrane are adjacent to Myofibrillar sarcoma and makeup 2 to 7% of the nuclei of a muscle fiber. The number of satellite cells depends on the type of muscle fiber, age, and species. The amount of these cells varies at different ages; in the neonatal, adult, and older mice, they make up 30%, 4%, and 2% of the muscle nuclei, respectively, and as they age, the decrease in satellite cells increases the number of muscle nuclei. It becomes glycolytic fibers. The migration capacity of satellite cells depends on the integrity and integrity of the cell's basement membrane. After rupture (high-intensity destruction) of the basement membrane by muscle damage, satellite cells migrate to adjacent damaged myofibrils using tissue connections, but only if tissue damage is limited. And if there is no rupture in the basal lamina, the satellite cells move to the affected area from the beginning of the healthy myofibril section (below the membrane) to participate in the repair of muscle tissue. Activation of satellite cells (six hours after muscle injury) rapidly increases the expression of the myoD gene, which is why this factor transcribes into adult skeletal muscle, activates and multiplies satellite cells. They take into account. The amount of myoD transcription factor mRNA varies at different ages and is more pronounced in fast-twitch muscles. In animal models, the amount of myoD gene protein is lower in the fast and slow-twitch muscles of older mice. This value is lowest in the horseshoe muscle and therefore the response of the horseshoe myoD gene (slow-twitch muscle) is also lower than that of the Plantaris muscle (fast-twitch muscle). In addition, the effects of supplements on myoD gene expression in muscle have not been studied, but in a study, the protective effect of organic Spirulina supplementation in preventing the destruction of hereditary structures in mice has been shown and appears. Consumption of this supplement is a good way to prevent injuries caused by oxidative stress in muscle, and most of the research done on this gene has paid less attention to slow-twitch and fast-twitch muscle contraction and experimental models (animal and human). While the protocols for this research were either endurance or the measurement times after physical activity was not appropriate. With this description, the aim of this study was to compare the effects of organic and inorganic supplementation on myoD gene expression in slow-twitch muscle after a session of high-intensity resistance activity to determine whether organic and inorganic supplementation on myoD gene expression. In slow muscle, does the contraction of the horseshoe muscle have different effects after a session of high-intensity resistance activity?</span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="tab-stops:10.5pt"><span style="line-height:115%"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:9.5pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:#0070c0">Methods:</span></span></span></span></b><b> </b><span style="font-size:9.5pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:black">In this experimental study, 40 Wistar rats with an average weight of 100-200 g were prepared and in the control groups (number = 10), exercise (number = 10), Spirulina + exercise (number = 10), glutamine + exercise (number = 10) were divided. Mice in the exercise group performed a two-week exercise program of 3 days per week of walking on a sloping surface (4 sets, 5 repetitions, 30 seconds rest between repetitions), and the supplement + exercise group performed a supplement program five days before the main protocol. They consumed half a gram/kg of body weight once a day. The obtained data were evaluated by t-test.</span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="tab-stops:10.5pt"><span style="line-height:115%"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:9.5pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:#0070c0">Results: </span></span></span></span></b><span style="font-size:9.5pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:black">The results showed that the relative expression of myoD gene in slow-twitch muscle tissue after taking an organic Spirulina supplement was significant after performing a session of high-intensity resistance activity (P </span></span></span></span><span style="font-size:9.5pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:black">≤</span></span></span></span><span style="font-size:9.5pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:black">0.001). While in slow muscle contraction, the change in expression after glutamine and resistance exercise is no different from the control group (P = 0.245).</span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="tab-stops:10.5pt"><span style="line-height:115%"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:9.5pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:#0070c0">Conclusion: </span></span></span></span></b><span style="font-size:9.5pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:black">In general, it can be concluded that the use of organic supplements is a good way to prevent reduced expression and the amount of damage to muscle fibers after high-intensity resistance exercise. These results may be due to possible injuries after high-intensity resistance exercise in slow-twitch fibers, or they may indicate a response to the development of adaptations related to the performance of resistance training in such fibers. It is important to note that in the Spirulina organic supplement group, prior to a high-intensity resistance activity session, myoD gene expression was lower than in high-intensity resistance activity without supplementation. Taking an organic supplement is a good way to prevent reduced expression and the amount of damage to muscle fibers. So far, the effects of glutamine and Spirulina supplements on the expression of myoD gene in muscle have not been studied, but a study has shown the protective effect of organic Spirulina supplementation in preventing DNA damage in mice. Taking this supplement is a good way to prevent injuries caused by oxidative stress in the muscle. Since in each study, there are limitations that can affect the results of the study, so this study also had such limitations that include not determining some of the basic physiological capacities in resting and training states of mice, lack of Determining the effects of organic and inorganic supplements on the expression of the target gene independently and without performing intense one-session resistance activity, no measurement of morphological changes in adult male Wistar rats, no measurement of protein content by the method Western blot and lack of control over the activity of adult male Wistar rats were in cages. According to the results of the study, it is suggested that in combination with high-intensity resistance activity, it is better to use appropriate supplements such as organic Spirulina supplement to increase the expression of myoD gene, which may be affected by the amount of damage to muscle fibers.</span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="tab-stops:10.5pt"><span style="line-height:115%"><span style="font-family:Calibri,sans-serif"><span style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"><span style="color:black"></span></span></span></span></span></span></span></span></span></span>
اسپیرولینا, ژن MyoD, عضله کند انقباض, آسیب عضلانی
Spirulina, Myod Gene, Slow-Twitch Muscle, Muscle Injury
1
11
http://rjms.iums.ac.ir/browse.php?a_code=A-10-6360-1&slc_lang=fa&sid=1
Ameneh
Zandi
آمنه
زندی
3900319475328460071746
3900319475328460071746
No
PhD Student in Sports Physiology, Department of Physical Education and Sport Sciences, Islamic Azad University, Damghan Branch, Damghan, Iran
دانشجوی دکتری فیزیولوژی ورزشی، گروه تربیت بدنی و علوم ورزشی دانشگاه آزاد اسلامی واحد دامغان، دامغان، ایران
Tahereh
Bagherpoor
طاهره
باقرپور
bagherpoor_ta@yahoo.com
3900319475328460071747
3900319475328460071747
Yes
Assistant Professor, Department of Exercise Physiology, Damghan Branch, Islamic Azad University, Damghan, Iran
استادیار، گروه فیزیولوژی ورزش، واحد دامغان، دانشگاه آزاد اسلامی، دامغان، ایران
Nematolah
Nemati
نعمت ا...
نعمتی
3900319475328460071748
3900319475328460071748
No
Associate Professor, Department of Exercise Physiology, Damghan Branch, Islamic Azad University, Damghan, Iran
دانشیار، گروه فیزیولوژی ورزش، واحد دامغان، دانشگاه آزاد اسلامی، دامغان، ایران