Razi Journal of Medical Sciences

fa اثر تمرینات تناوبی با شدت بالا بر فاکتور مهارکننده تومورزایی و اینترلوکین-33 زنان مبتلا به دیابت نوع 2 The Effects of High-Intensity Interval Training on Suppression of Tumorigenicity 2 and Interleukin-33 in Women with Type 2 Diabetes فیزیولوژی ورزش Exercise Physiology پژوهشي Research زمینه و هدف: تمرینات ورزشی دارای اثرات مفید و ضدالتهابی است که موجب سرکوب التهاب سیستمی با درجه پایین در دیابت نوع 2 می‌شود. هدف از پژوهش حاضر بررسی اثر تمرین تناوبی با شدت بالا بر فاکتور مهارکننده تومورزایی و اینترلوکین-33 در زنان مبتلا به دیابت نوع 2 بود.  روش‌ کار: در این پژوهش نیمه تجربی و میدانی، 30 نفر از زنان مبتلا به دیابت نوع 2 با میانگین سنی 05/3 ±75/38، شاخص توده بدن 44/1±17/28 و گلوکز ناشتای بالاتر از 126 تا250 میلی‌گرم بر دسی لیتر به‌طور داوطلبانه در تحقیق حاضر شدند و بر اساس مقادیر هموگلوبین گلیکوزیله در دو گروه تمرین تناوبی (15 نفر) و کنترل (15 نفر) قرار گرفتند. گروه تمرین تناوبی تمرینات را به مدت دوازده هفته، سه جلسه در هفته گروه با 75 درصد ضربان قلب بیشینه شروع و تا شدت ۹0 درصد ضربان قلب بیشینه افزایش دادند. نمونه‌های خونی فاکتور مهار کننده تومورزایی و اینترلوکین 33 قبل و پس از 12 هفته تمرین، با روش الایزا اندازه‌گیری شد. به منظور بررسی آماری داده‌ها از آزمون شاپروویلک برای تعیین نرمالیته داده‌ها، از آزمون t وابسته برای  مقایسه میانگین‌های پیش آزمون و پس آزمون متغیرهای مورد مطالعه درون گروه‌ها و از آزمون کواریانس برای مقایسه بین گروه‌ها استفاده گردید. یافته‌ها: نتایج تحلیل داده‌ها با استفاده از آزمون تی همبسته نشان داد که 12 هفته تمرین اثرات معنی‌داری بر فاکتور مهار کننده تومورزایی و اینترلوکین 33 داشته است. مقادیر سرمی فاکتور مهار کننده تومورزایی پس از تمرین تناوبی نسبت به شرایط کنترل، کاهش معناداری داشته است. همچنین در گروه تمرین تناوبی، اینترلوکین 33  در مقایسه با گروه بی تمرین تفاوت معناداری را در پس آزمون نسبت به پیش آزمون نشان داد (05/0≥P). نتیجه‌گیری: نتایج تحقیق حاضر نشان می‌دهد تمرینات تناوبی می‌تواند باعث بهبود سطح گلوکز و کاهش درجه التهاب در بیماران مبتلا به دیابت نوع دو شود. Background & Aims: Diabetes is a metabolic disorder in which the body becomes insulin resistant and therefore the insulin produced cannot function normally. Among the long-term complications of this disease, the most common cause of death is cardiovascular disease, especially atherosclerosis (2). Researches has shown that the presence of levels of systemic inflammation, independent of glycemic control, contributes to the complications of diabetes, especially cardiovascular disease (3). Interleukin-33 and its receptor (ST2) play an important role in inflammation, infection, and autoimmune diseases. Interleukin-33 is known as "dual cytokine" and acts as a protective and detrimental effect or as a pro-inflammatory or anti-inflammatory factor as well as a nucleus transcription factor that depends on the disease model and cell type (7). Researches has shown that exercise training, with its effect on inflammation, plays a key role in improving inflammatory factors and insulin resistance in patients with type 2 diabetes and leads to a reduction in mortality from diabetes and its complications (9). Many studies have reported that exercise has anti-inflammatory effects in diseases such as obesity and cardiovascular disease and type 2 diabetes and improves metabolic disorders. Among the training methods, most of the studies on the effect of exercise on cardiovascular risk factors used continuous and often long-term aerobic activity in their studies. In contrast, interval training is a training method that requires less time with high intensity and low volume and improves diseases and body composition (10). Methods: The statistical population of the present study consists of women 35 to 50 years with type 2 diabetes in Esfahan that were selected purposefully and divided randomly into two groups of experimental and control. Inclusion criteria included type 2 diabetes, fasting blood sugar above 126 to 250 mg / dL, no history of cardiovascular, pulmonary, renal disease, no drug addiction, no regular exercise during the last 6 months And there were no skeletal abnormalities. To measure the height of the subjects, the SECA model's gauge was used, to calculate the weight of the subjects, the GLMOR brand digital scale was used and to control the heart rate, the polar pacemaker was used. Regarding the measurement of blood variables, ELISA laboratory kit was used to measure serum levels of Suppression of  Tumorigenicity 2  and interleukin 33. Rockport test was also used to estimate the aerobic capacity of patients. diet 24-hour dietary recalls was used to control diet; Anthropometric and physiological measurements of the subjects were performed. Fasting blood sample were taken from the subjects 24 hours before the first session and 48 hours after the last exercise session. The interval Training conducted including 12 weeks, three sessions in a week with 75 to 90% of the maximum heart rate. Also, the collected information was presented in two levels of descriptive and inferential statistics. Results: The results of one-way ANOVA demonstrated that there was no significant difference in the values of anthropometric features at the beginning of the period between groups (P <0.05). This point is a sign of homogeneity of groups. The anthropometric and physiological characteristics of the subjects are shown in Table 1. The results of data analysis using correlated t-test showed that 12 weeks of training had significant effects on body weight loss, Suppression of  Tumorigenicity 2, Interleukin-33 and Vo2max. Also the results of data analysis using ANCOVA showed that serum levels of Suppression of  Tumorigenicity 2 after high-intensity interval training had a significant decrease compared to control conditions. Conclusion: Disorders of lipid and glucose metabolism are characteristic of type 2 diabetes. The link between ST2 circulation and metabolic disorders in diabetes is not yet fully understood. The IL-33/ST2 signaling pathway may be involved in the development of type 2 diabetes through inflammation. Its mechanism is as follows: Interleukin 33 has a protective role against glucose metabolism and obesity in obese mice and a Suppression of  Tumorigenicity 2 for interleukin-33. Binding of the two stimulates the activity of Th2 cells and Th2 increases inflammation by releasing inflammatory cytokines. Therefore, increased levels of Suppression of  Tumorigenicity 2 in diabetes may impair the protective effects of interleukin 33 (20). Analyzes show links between immune status and exercise. Knowing the function of the immune system in the context of exercise and the mechanism regulation of nerve endocrine glands and the physiological significance of these changes is invaluable.  Recent findings show that high-intensity interval training is an useful way to reduce fat and inflammation and cause a negative energy balance (21). Overall, the present study showed that regular interval exercise can alleviate the inflammatory response and Control blood sugar in patients with type 2 diabetes. Therefore, exercise intervention can minimize the incidence of complications associated with type 2 diabetes. As a result, exercise training is widely recommended as a treatment option for these people.    تمرین تناوبی, فاکتور مهارکننده تومور زایی, اینترلوکین-33 , دیابت نوع 2 Interval training, Suppression of Tumorigenicity 2, Interleukin-33, Type 2 Diabetes 49 58 http://rjms.iums.ac.ir/browse.php?a_code=A-10-5481-1&slc_lang=fa&sid=1 Laleh Naderi لاله نادری 3900319475328460065247 3900319475328460065247 No PhD Student, Department of Physical Education and Sport Sciences, Najafabad Branch, Islamic Azad University, Najafabad, Iran دانشجوی دکتری فیزیولوژی ورزشی، گروه تربیت بدنی و علوم ورزشی، واحد نجف‌آباد، دانشگاه آزاد اسلامی، نجف‌آباد، ایران Jamshid Banaei Borojeni جمشید بنایی بروجنی jamshid.banaii@gmail.com 3900319475328460065248 3900319475328460065248 Yes Assistant professor, Sport Medicine Research Center, Najafabad Branch, Islamic Azad University, Najafabad, Iran استادیار، مرکز تحقیقات طب ورزشی، واحد نجف‌آباد، دانشگاه آزاد اسلامی، نجف‌آباد، ایران Mehdi Kargarfard مهدی کارگرفرد 3900319475328460065249 3900319475328460065249 No Professor, Department of Exercise Physiology,faculty of sport sciences, University of Isfahan, Iran استاد، گروه فیزیولوژی ورزش، دانشکده علوم ورزشی، دانشگاه اصفهان، دانشگاه اصفهان، اصفهان، ایران Saeed Keshavarz سعید کشاورز keshavarz1357@gmail.com 3900319475328460065250 3900319475328460065250 No Assistant professor, Sport Medicine Research Center, Najafabad Branch, Islamic Azad University, Najafabad, Iran استادیار، مرکز تحقیقات طب ورزشی، واحد نجف‌آباد، دانشگاه آزاد اسلامی، نجف‌آباد، ایران