Razi Journal of Medical Sciences
مجله علوم پزشکی رازی
RJMS
Medical Sciences
http://rjms.iums.ac.ir
39
journal39
2228-7043
2228-7051
en
jalali
1400
1
1
gregorian
2021
4
1
28
2
online
1
fulltext
fa
تغییر مقادیر IGF1 و Akt قلبی رتهای مبتلا به انفارکتوس میوکارد در اثر شدتهای گوناگون تمرین تناوبی
Changes of cardiac IGF-1 and Akt levels in rats with myocardial infarction due to different intensities of interval training s
فیزیولوژی ورزش
Exercise Physiology
پژوهشي
Research
<strong><span style="color:#0070c0;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">زمنیه و هدف:</span></span></span></strong> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">بیماری قلبی­-عروقی یکی از عامل­های اصلی مرگ­ومیر در همه­ی کشور­های دنیا به شمار می­رود و فعالیت ورزشی یکی از مهم­ترین اقدامات بالینی برای پیشگیری و درمان بیماری­های قلبی­-عروقی است</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">.</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> لذا، هدف از انجام پژوهش حاضر، تبیین تغییرات مقادیر </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">IGF1</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> و </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">Akt</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> قلبی موش­های مبتلا به آنفارکتوس میوکارد (</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">MI</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">-</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;"> Myocardial Infarction-</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">) پس از شش هفته تمرین تناوبی با شدتهای گوناگون بود. </span></span></span><br>
<strong><span style="color:#0070c0;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">روش کار:</span></span></span></strong> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">در این مطالعه نیمه تجربی، ابتدا 55 سر موش­­ نر نژاد ویستار 10 هفته­ای با وزن 250 تا 300 گرم تحت عمل جراحی بستن شریان کرونری </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">LAD</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> قرار گرفتند و سپس توسط اکوکاردیوگرافی ایجاد </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">MI</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> تأیید شد. چهار هفته پس از جراحی، موشهای مبتلا به </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">MI</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">، به طور تصادفی در گروه­های تمرین ­ورزشی تناوبی با شدت­های کم (60 </span></span></span><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">–</span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> 55 درصد </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">VO<sub>2max</sub></span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">)، متوسط (70-65 درصد </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">VO<sub>2max</sub></span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">)، زیاد (90-85 درصد </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">VO<sub>2max</sub></span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">)، </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">MI</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> بی­تمرین (</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">MI-SED</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">)</span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> و </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">Sham</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> قرار گرفتند و پروتکل­های تمرین ورزشی را به مدت شش هفته و پنج جلسه در هفته اجرا کردند. در نهایت برای سنجش مقادیر پروتئین </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">Akt</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> و</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">IGF1 </span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> از روش الایزا استفاده گردید</span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">.</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">داده­ها با آزمون </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">ANOVA</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> یک­طرفه و آزمون بونفرونی تجزیه و تحلیل شدند. </span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"></span></span></span><br>
<strong><span style="color:#0070c0;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">یافته ­ها:</span></span></span></strong> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">نتایج نشان داد بین گروه­های مورد مطالعه در مقادیر پروتئین­های </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">IGF1</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> (</span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">279/0 =</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">P</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">) و </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">Akt </span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> (</span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">063/0 =</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">P</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">) تفاوت معناداری وجود نداشت.</span></span></span><br>
<strong><span style="color:#0070c0;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">نتیجه ­گیری:</span></span></span></strong> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">براساس نتایج این پژوهش، بین تأثیر شدت­های مختلف فعالیت ورزشی تناوبی بر پروتئین­های </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">IGF1</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> و </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">Akt</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> تفاوتی وجود ندارد. با این حال، برای روشن­تر شدن سازوکار اثر شدت­های گوناگون تمرین تناوبی بر پروتئین­های </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">IGF1</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> و </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">Akt </span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">انجام پژوهش­های بیشتر ضرورت دارد.</span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"></span></span></span><br>
<strong>Background and Aims: </strong>Cardiovascular disease is one of the leading causes of death in all countries of the world and exercise is one of the most important clinical measures for the prevention and treatment of cardiovascular disease. Therefore, the aim of this study was to explain the changes in IGF1 and cardiac Akt levels in mice with myocardial infarctionAims: Despite advances in healthcare practices, cardiovascular disease remains the leading cause of death in all countries of the world. Among patients who survive myocardial infarction (MI), which is a direct result of cardiovascular disease, the extent of injury prevention depends on the size of the myocardium, which is destroyed by ischemic injury (infarct size). Therefore, it is believed that a significant reduction in the size of myocardial infarction reduces morbidity and subsequent mortality. Myocardial ischemia is caused by the cessation of blood flow, followed by the cessation of blood supply and nutrients to the heart muscle. These conditions cause specific physiological changes and a series of events in myocardial cells that if this process continues for a long time, the death of the heart muscle is inevitable. Therefore, any change, however small, in improving and increasing the life expectancy of heart patients can have a significant impact on promoting community health. Studies have shown that exercise is one of the most important clinical measures for the prevention and treatment of cardiovascular disease. Physical activity not only reduces cardiovascular risk factors such as diabetes and hypertension and thus helps prevent heart disease, but also seems to improve the condition and functional outcomes of patients with heart disease. It does better. Cardiovascular benefits Exercise involves several factors, including important general effects on skeletal muscle, peripheral arteries, and metabolism, as well as beneficial changes within the myocardium. In the study of exercise-related signaling pathways, it has been acknowledged that physiological hypertrophy is first achieved through insulin-like growth factor-1 (IGF-1) or the IGF-1-PI3K-Akt axis. IGF-1 is produced by the liver and to a lesser extent by the heart. Exercise also stimulates the hepatic secretion of IGF-1 into the bloodstream and stimulates cardiac expression of IGF-1. In heart tissue, IGF-1 binds to its tyrosine kinase receptor, IGF-1R, and activates the PI3K-Akt cascade. Protein serine threonine kinase (STK) is the main downstream stimulus of PI3k and is phosphorylated (activated) in physiological hypertrophy of the heart. The effects of Akt1 on the heart are numerous, though generally considered beneficial. These effects include: inhibiting cardiac myocyte death, improving calcium control, heart growth factor, and metabolism. In general, it can be stated, due to the various biological effects attributed to IGF-1 and also because of the wide therapeutic capacity for this factor and also due to the lack of research in which the expression of this factor to Along with the messaging pathway involved in physiological cardiac hypertrophy (Akt1) under the influence of various intensities of intermittent exercise, and finally due to the importance of health care in cardiovascular patients, the present study seeks to design an intermittent exercise protocol with intense Answers the question of whether the levels of IGF-1 and Akt in rats with myocardial infarction differ in different intensities of intermittent exercise after six weeks?<br>
<strong>Methods:</strong> The present study with the code RHC.AC.IR.REC.1393.28 was approved by the Ethics Committee of Shahid Rajaei Cardiovascular Training, Research, and Treatment Center. It is fundamental-developmental in terms of purpose and experimental in terms of implementation and based on the level of supervision and degree of control, it is a type of laboratory research. In this experimental study, 55, 10-week-old male Wistar rats weighing 250 to 300 g were purchased from the Pasteur Institute of Iran. During the interventions, rats were kept in three cages in each cage with free access to water and food packages, and according to the 12-hour sleep-wake cycle at the Experimental Research Center of Shahid Rajaei Heart Hospital. The room temperature was maintained in the range of 37°C, and efforts were made to maintain and work with animals in accordance with the recommendations of the Laboratory Animal Protection Act (NIH). In the present study, Direct intervention method was used to induce MI in rats in which the left-anterior descending-coronary-artery (LAD) of rats was blocked by suture. Finally, surviving rats with MI were randomly divided into four groups (6 heads): low-intensity training (LIT), moderate-intensity training (MIT), high-intensity training (HIT), and myocardial infarction and Sham. Finally, the ELISA method was used to measure the levels of Akt and IGF1 protein. Shapiro-Wilk test was used to determine the normality of the data and one-way ANOVA and Bonferroni post hoc test at a significance level of α = 0.05 were used to analyze the hypotheses. SPSS software version 19 was used for statistical analysis.<br>
<strong>Results:</strong> The results showed that there was no significant difference in the levels of IGF1 and Akt proteins between the studied groups (p≥0.05).<br>
<strong>Conclusion: </strong>It can be concluded that there was no difference between the effect of different intensities of Interval Training on IGF1 and Akt proteins, therefore, in order to clarify the mechanism of the effect of different intensities of Interval Training on IGF1 and Akt proteins, more studies are needed.<br>
<br>
آنفارکتوس میوکارد, تمرین تناوبی شدید,IGF1,Akt
Myocardial Infarction, High Intensity Interval Training, IGF1, Akt.
44
55
http://rjms.iums.ac.ir/browse.php?a_code=A-10-5354-1&slc_lang=fa&sid=1
Reyhane
Zoghi
ریحانه
ذوقی
r.zoghi@ut.ac.ir
3900319475328460056879
3900319475328460056879
No
Kish International Campus, University of Tehran, Kish, Iran
پردیس بینالمللی دانشگاه تهران، کیش، ایران
Abbasali
Gaeini
عباسعلی
گائینی
aagaeini@ut.ac.ir
3900319475328460056880
3900319475328460056880
No
Professor, Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran
دانشگاه تهران، تهران، ایران
Reza
Nuri
رضا
نوری
nuri_r7@ut.ac.ir
3900319475328460056881
3900319475328460056881
Yes
Kish International Campus, University of Tehran, , Kish, Iran
پردیس بین المللی دانشگاه تهران، کیش، ایران
Mohammad
Hemati Nafar
محمد
همتی نفر
mhemati.66@ut.ac.ir
3900319475328460056882
3900319475328460056882
No
Assistant Professor of Exercise Physiology, Faculty of Educational Sciences and Psychology, Department of Sport Sciences, Shiraz University, Shiraz, Iran
بخش علوم ورزشی، دانشگاه شیراز، شیراز، ایران