Razi Journal of Medical Sciences

fa تاثیر تمرین مقاومتی کم شدت همراه با محدودیت جریان خون (BFR) تناوبی و تداومی بر P38 و PI3K در مردان میانسال غیرفعال The effect of low intensity resistance training with intermittent and continuous blood flow restriction (BFR) on P38 and PI3K in inactive middle-aged men فیزیولوژی ورزش Exercise Physiology پژوهشي Research زمینه و هدف: روش زندگی کم تحرک با گسترش رویدادهای بالینی خطرناک در افراد میانسال همراه است. هدف از انجام این تحقیق، تبیین تاثیر تمرین مقاومتی کم شدت همراه با محدودیت جریان خون (BFR) تناوبی و تداومی بر P38 و PI3K در مردان میانسال غیرفعال بود.   روش کار: در این تحقیق نیمه تجربی، 45 مرد میانسال سالم غیرفعال (سن 40 تا 65 سال) به طور تصادفی به سه <a name="_Hlk45702907">گروه تمرین مقاومتی </a>همراه با محدودیت جریان خون (BFR) تناوبی، تمرین مقاومتی همراه با BFR تداومی، تمرین مقاومتی بدون BFR تقسیم شدند. گروههای تمرین مقاومتی کم شدت همراه با BFR تناوبی و تداومی، سه جلسه تمرین در هفته همراه با محدودیت جریان خون با فشار کاف 110 میلیمتر جیوه در ناحیه ران طی 8 هفته اجرا کردند. همچنین گروه تمرین مقاومتی، 3 جلسه در هفته بدون BFR تمرین کردند. در پایان مداخله، نمونه خونی گرفته شد. دادهها به روش t وابسته، تحلیل واریانس یکطرفه و آزمون تعقیبی توکی در سطح معنیداری 05/0p< تجزیه و تحلیل شدند. یافته ها: نتایج نشان داد که تمرین مقاومتی کم شدت همراه با BFR تناوبی و تداومی منجر به کاهش معنیدار میزان p38 (001/0p=) و افزایش معنیدار سطوح PI3kinase نسبت به گروه تمرین مقاومتی بدون BFR در مردان میانسال شد (001/0p=) همچنین بین تاثیر دو روش تمرین همراه با BFR تفاوت معنیداری مشاهده نشد (05/0<p). نتیجه‌گیری: بهنظر میرسد تمرین مقاومتی کم شدت همراه با محدودیت جریان خون (BFR) تناوبی و تداومی میتواند به بهبود شاخصهای قلبی-عروقی در مردان میانسال غیرفعال کمک نماید و بین دو روش تفاوت وجود ندارد. Background: A sedentary lifestyle is associated with the spread of dangerous clinical events in middle-aged people. The most serious health problems of sedentary lifestyle are obesity, type 2 diabetes and cardiovascular diseases. In the cardiovascular system, cellular responses to various stimuli are mediated through coordinated signaling pathways. Mitogen-activated protein kinases (MAPKs) are a family of known proteins that play an important role in these signaling events. However, this family also has good and bad members who can protect or damage the heart. P38 is expressed in heart cells. In cultured cells of heart tissue, P38 activity induces myocyte hypertrophy and apoptosis, as well as increased cytokine production. Also, in the cardiovascular system, special emphasis has been placed on the role of phosphoinositide-3 kinase (PI3K) in health and disease. Studies have shown that the PI3K-Akt signaling cascade is associated with cardiac hypertrophy. Resistance training improves strength and muscle mass and cardiovascular function in middle-aged and elderly people. However, many inactive people are reluctant to exercise at moderate or high intensity, and high intensity training may not be appropriate for these people and may be associated with injury. Therefore, the effectiveness of alternative training methods has been investigated. Among these methods is low intensity resistance training with blood flow restriction (BFR). Research has reported an increase in cardiovascular function after resistance training with BFR. it may be possible to achieve an optimal training method for inactive middle-aged people, for treatment and to prevent the effects of aging, by determining the effect of blood flow restriction with low intensity resistance training on cardiovascular parameters. However, the effects of intermittent and continuous blood flow restriction on inactive cardiovascular parameters are not known, Therefore, the aim of this study was to evaluate the effect of low intensity resistance training with intermittent and continuous blood flow restriction (BFR) on P38 and PI3K in inactive middle-aged men. Methods: This is a semi-experimental study. This research was conducted on inactive healthy middle-aged men. 45 inactive healthy middle-aged men (40-65 years) were randomly divided into three groups include resistance training with intermittent BFR, resistance training with continuous BFR, Resistance training without BFR. Low-intensity resistance training groups with intermittent and continuous BFR performed 3 sessions per week blood flow restriction with a cuff pressure of 110 mm Hg in the thigh area for 8 weeks. also, the resistance training group performed the exercise, 3 sessions per week without BFR. At the end of the intervention, blood was taken. The p38 and PI3kinase levels were measured by ELISA kit. Data were analyzed by dependent t test, One-way ANOVA and Tukey post hoc test at the p<0.05. Results: Data analysis showed that there was a difference between changes of p38 and PI3kinase levels in middle-aged men in different research groups (p=0.001). The results showed that low-intensity resistance training with intermittent and continuous BFR led to significant decrease in p38 (p=0.001), as well as significant increase in PI3kinase levels compared to the resistance training group without BFR in middle-aged men (p=0.001). There was no significant difference between the effect of two methods training with BFR (p<0.05). Conclusion: The results of our study suggest that both low-intensity resistance training with intermittent and continuous BFR may have greater cardiac benefits for middle-aged people than low-intensity resistance training. The findings of this study were consistent with the results of Previous research [31-29]. Regarding the mechanisms of change of MAPKs following exercise with BFR, it seems that part of the activation of this pathway is due to increased GH secretion, fluid exchange between blood and active muscle tissue, and BFR-induced hypoxia [26-28]. Regarding the mechanisms affecting PI3K changes, it has been reported that high expression of IGF-1 or IGF receptors activates PI3K (p110α) and induces physiological hypertrophy of the heart and myocardial infarction. Therefore, it is possible that low-intensity resistance training with intermittent and continuous BFR and low-intensity resistance training without BFR in the present study led to an increase in PI3K by affecting IGF-1 levels. There were some limitations in the present study, such as the lack of measurement of other cardiovascular indicators in inactive middle-aged men. Also, Measurement of cardiac function indices can more clearly show the effects of resistance training with BFR on the cardiovascular system in inactive middle-aged people. According to the results, it seems that low intensity resistance training with intermittent and continuous blood flow restriction (BFR) can help improve cardiovascular performance in inactive middle-aged men, and there is no difference between the two methods. . تمرین مقاومتی, محدودیت جریان خون تداومی, محدودیت جریان خون تناوبی, شاخص‌های قلبی-عروقی, مردان میانسال غیرفعال Resistance training, Continuous blood flow restriction, Intermittent blood flow restriction, Cardiovascular indices, Inactive middle-aged men 178 187 http://rjms.iums.ac.ir/browse.php?a_code=A-10-2723-3&slc_lang=fa&sid=1 Sara Nasiri Semnani سارا نصیری سمنانی sara.nasiri64@gmail.com 3900319475328460053410 3900319475328460053410 No Department of Exercise Physiology, Shahid Rajaee Teacher Training University Faculty of Science, Tehran, Iran گروه فیزیولوژی ورزشی، دانشگاه تربیت دبیر شهید رجایی، تهران، ایران. Majid Kashef مجید کاشف kashef1327@gmail.com 3900319475328460053411 3900319475328460053411 Yes Shahid Rajaee Teacher Training University Faculty of Science, Tehran, Iran دانشگاه تربیت دبیر شهید رجایی، تهران، ایران Gholam Reza Partovi غلامرضا پرتوی Gholamrezapartovi33@gmail.com 3900319475328460053412 3900319475328460053412 No Department of Exercise Physiology, Shahid Rajaee Teacher Training University Faculty of Science, Tehran, Iran گروه فیزیولوژی ورزشی، دانشگاه تربیت دبیر شهید رجایی، تهران، ایران. Fereshteh Shahidi فرشته شهیدی Shahidi.11@gmail.com 3900319475328460053413 3900319475328460053413 No Department of Exercise Physiology, Shahid Rajaee Teacher Training University Faculty of Science, Tehran, Iran گروه فیزیولوژی ورزشی، دانشگاه تربیت دبیر شهید رجایی، تهران، ایران.