Razi Journal of Medical Sciences
مجله علوم پزشکی رازی
RJMS
Medical Sciences
http://rjms.iums.ac.ir
39
journal39
2228-7043
2228-7051
en
jalali
1400
2
1
gregorian
2021
5
1
28
3
online
1
fulltext
fa
تاثیر تمرین تناوبی شدید بر بیان ژن FGF-21 در عضله تاکننده دراز انگشت پا(FHL) و سطح سرمی TNF-α در موش های صحرایی دیابتی
The effect high-intensity interval training on FGF-21 expression in flexor hallucis longus muscle and serum TNF-α levels in diabetic rats
فیزیولوژی ورزش
Exercise Physiology
پژوهشي
Research
<strong><span style="color:#0070c0;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">زمینه و هدف: </span></span></span></strong><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">دیابت نوع 2 یک اختلال متابولیک شایع و گسترده در دنیا است. نامتعادل بودن الگوی متابولیکی، مقاومت به انسولین و افزایش سطح شاخصهای التهابی در بیماران دیابتی بیان شده است. افزایش مایوکاینها به دلیل تمرینات ورزشی میتواند در تنظیم فرآیندهای متابولیکی و التهابی موثر باشند. هدف از این پژوهش بررسی تأثیر 6 هفته تمرین تناوبی شدید بر بیان ژن</span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> فاکتور رشدی فیبروبلاست 21 (</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">Fibroblast growth factor</span></span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">21</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">) در </span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">عضله تاکننده دراز انگشت پا (</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">Flexor</span></span></span></span> <span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">Hallucis Longus- FHL</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">) </span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">و مقدار سرمی </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">TNF-α</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> در</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">موشهای صحرایی دیابتی بود.</span></span></span><br>
<strong><span style="color:#0070c0;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">روش کار:</span></span></span></strong><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> در این مطالعه تجربی، تعداد</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">48 موش نر صحرایی نوع ویستار بهطور تصادفی به 4 گروه دیابت، تمرین، دیابت+تمرین و کنترل تقسیم شدند. پروتکل، تمرین بر روی نوارگردان با سرعت و شیب پیش رونده در طی 6 هفته بود. در پایان هفتهی ششم پس از استخراج نمونه، بیان ژن </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">FGF-21</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> با روش </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">Real time PCR</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> اندازهگیری شد. تجزیه تحلیل دادهها با استفاده از آزمون تحلیل واریانس یک طرفه در سطح معناداری (05/0</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">p<</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">) انجام شد. </span></span></span><br>
<strong><span style="color:#0070c0;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">یافتهها:</span></span></span></strong><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> نتایج بدست آمده نشان میدهد که 6 هفته تمرین تناوبی شدید بر بیان ژن </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">FGF-21</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> عضله </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">FHL</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> موشهای دیابتی تأثیر معناداری ندارد (</span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">095</span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">/0</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">p=</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> ). درحالیکه کاهش التهاب و عوامل التهابی نظیر </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">TNF-α</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> در اثر تمرین تناوبی شدید را نشان داده شد (</span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">0</span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">00/0</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">p=</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">). </span></span></span><br>
<strong><span style="color:#0070c0;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">نتیجهگیری:</span></span></span></strong><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> تمرین تناوبی شدید موجب کاهش التهاب و مانع تحلیل عضله میشود. میتوان از تمرین تناوبی به عنوان یک رویکرد درمانی برای مدیریت دیابت استفاده کرد.</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;"></span></span></span></span>
<strong>Background & Aims:</strong> Type 2 diabetes is one of the metabolic diseases that is associated with partial or absolute insulin deficiency, high blood glucose and disorders of carbohydrate, fat and protein metabolism. This condition is characterized by a persistent rise in blood sugar and can be caused by a disorder in the secretion or action of insulin, or both. Metabolic pattern imbalance, insulin resistance, and increased levels of inflammatory markers have been reported in diabetic patients. The effects of diabetes threaten the lives of millions of people around the world every year. A healthy lifestyle, proper nutrition and regular exercise are the most important factors in preventing or reducing the effects of diabetes. main imperfection in the pathology of type 2 diabetes include insulin resistance in muscle and liver.<br>
Skeletal muscle is considered to be the largest organ in the body in non-obese individuals, and as an active endocrine organ it releases myokines. Myokines have systemic effects on the liver, adipose tissue, brain and immune system. They can be effective in regulating metabolic and inflammatory processes. Fibroblast growth factor-21 is known as a member of the family of fibroblast growth factors and has recently been introduced as a factor in regulating the metabolic process.<br>
Because diabetes medications have many limitations, including high cost, and side effects, many strategies have been proposed to control insulin and reduce insulin resistance in diabetes. Regular exercise and following a healthy diet is a good strategy to treat and reduce costs in many metabolic disorders, including type 2 diabetes and obesity. High-Intensity Interval Training has recently been considered as a suitable training option, not only among athletes but also among those who are limited in time. Exercise reduces inflammation and reduces cell death caused by diabetes in the body's cells by activating intracellular and extracellular pathways. Increased myokines due to exercise can be effective in regulating metabolic and inflammatory processes. The aim of this study was to investigate the effect of 6 weeks of High-Intensity Interval Training on FGF21 Expression in Flexor Hallucis Longus muscle tand serum TNF-α Levels of diabetic rats.<br>
<strong>Methods:</strong> In this experimental study, 48 Wistar male rats weighing 150±10g were randomly divided into 4 groups of Diabetes (n = 10), Control (n = 10), Diabetes +Training (n = 12) and Training (n = 12). All animals were kept in separate cages under the similar conditions. In this study, For induction of experimental diabetes Wistar rats received streptozotocin as a single dose of 50 mg / kg body weight by intraperitoneal injection. The training program consisted of 10 sets of 1 minute running (2 minutes rest between each set) on a treadmill with gradually increasing speed and incline, for 6 weeks and 3 training sessions per week. Running speed gradually increased from 18 to 31 m / min over 6 weeks and the treadmill slope increased from 2 to 10 ° (50 to 110% VO2max). At the end of the sixth week, after FHL muscle tissue sampling, FGF-21 gene expression was measured by Real time PCR and serum TNF-α level was measured by ELISA using Diaclone kits from France. In the inferential statistics section, Shapiro-Wilk test was used to the normality of data. equality of variances with Levene test was also measured. Findings were evaluated at a significance level of 95% (p <0.05). SPSS software version 22 was used for statistical analysis of data.<br>
<strong>Results:</strong> The results showed that 6 weeks of High-Intensity Interval Training did not have a significant effect on FGF-21 gene expression of FHL muscle in diabetic rats (p = 0.095). However, the reduction of inflammation and inflammatory factors such as TNF-α was shown due to High-Intensity Interval Training (p = 0.000).<br>
<strong>Conclusion:</strong> Considering all the above and also the results of statistical analysis, it was found that High-Intensity Interval Training has no significant effect on the expression of FGF-21 gene in FHL muscle tissue of diabetic rats. But regular exercise, in addition to weight control, has anti-inflammatory effects and suppresses systemic inflammation and muscle atrophy. Tnf-α is one of the major pro-inflammatory cytokines that causes inflammation. The results of the present study showed that High-Intensity Interval Training reduces the negative effects of diabetes on the cardiovascular system and health by activating various cellular and molecular pathways and mechanisms. Serum Tnf-α levels, which are a factor in pro-inflammation and apoptosis, are reduced by High-Intensity Interval Training. However, much research is needed to substantiate the above results. In addition, High-Intensity Interval Training can be used as a therapeutic approach to diabetes management for patients who are unable to perform continuous exercise for a variety of reasons.
تمرین تناوبی شدید, دیابت, FGF-21, بیان ژن, مایوکاین,TNF-α
High Intensity Interval Training, diabetes, FGF-21, expression gene, myokines, TNF-α
9
18
http://rjms.iums.ac.ir/browse.php?a_code=A-10-4692-1&slc_lang=fa&sid=1
Mohammad Ayoub
Shirzadi
محمدایوب
شیرزادی
std_ayoubshirzadi@khu.ac.ir
3900319475328460057594
3900319475328460057594
No
Kharazmi University, Tehran, Iran
دانشگاه خوارزمی، تهران، ایران
Neda
Khaledi
ندا
خالدی
N.khaledi@khu.ac.ir
3900319475328460057595
3900319475328460057595
Yes
Kharazmi University, Tehran, Iran
دانشگاه خوارزمی، تهران، ایران