fa اثر جایگزینی miR-34a در مهار رشد رده سلول سرطانی مری خون و انکولوژی Hematology & oncology پژوهشي Research <h2 dir="RTL"><strong><span style="color:#0070C0;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">زمینه و هدف: </span></span></span></strong><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">سرطان مری ششمین علت مرگ و میر ناشی از سرطان در سرتاسر جهان به علت بدخیم بودن و پیش آگهی ضعیف است. </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;">miR-34a</span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> یک سرکوبگر تومور می‌باشد. خانواده </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;">miRNA-34</span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> در سرکوب کردن تومور و مسیرهای انکوژنی در برخی سرطان‌ها نقش دارد. منظور از این تحقیق این است که پس از جایگزین کردن </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;">miR-34a</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">تغییرات بیان ژن </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;">Vimentin</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">معیین گردد و از طرفی تأثیر جایگزین کردن این میکرو</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-size:10.0pt;">RNA</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">بر روی تکثیر سلولی مشخص گردد.</span></span></span><br> <strong><span style="color:#0070C0;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">روش کار:</span></span></span></strong><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> بعد از کشت دادن رده سلولی </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">TE-8</span></span></span></span><strong><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">،</span></span></span></strong> <span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">miR-34a</span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> در سلول‌های سرطانی مری جایگزین </span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">شد و بعد از جایگزینی </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">miR-34a</span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">، بیان ژن </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Vimentin</span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> با</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">روش</span></span></span> <span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">qRT-PCR</span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> مورد ارزیابی قرار گرفت. تکثیر سلولی در رده سلولی </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">TE-8</span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> در زمان و دوز مؤثر بعد از جایگزینی </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">mir-34a</span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> با روش</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;"> MTT</span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">مورد بررسی قرار گرفت.</span></span></span><br> <strong><span style="color:#0070C0;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">یافته‌ها:</span></span></span></strong><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">&nbsp; جایگزین کردن </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">miR-34a</span></span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">در&nbsp; رده سلولی </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">TE-8</span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> موجب</span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> کاهش معنی داری در بیان ژن بیان ژن </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Vimentin</span></span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">شد (0001/ 0 > </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">p</span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">). </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">mir-34a</span></span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">توانست (در دوز مؤثر بدست آمده) در 24 ساعت بطور چشمگیری تکثیر سلولی را کاهش دهد (0001/ 0 > </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">p</span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">). </span></span></span><br> <strong><span style="color:#0070C0;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">&nbsp;نتیجه‌گیری:</span></span></span></strong><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> احتمالا جایگزینی </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">miR-34a</span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> می‌تواند نقش درمانی در سرطان مری داشته باشد.</span></span></span><br> <br> <em><span style="font-style:normal;"><span style="color:windowtext;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"></span></span></span></span></em></h2> <strong>Background:</strong> Because of its malignancy and poor prognosis, esophageal cancer is the sixth cause of death around the world. MiR-34a is a tumor suppressor. The miRNA-34 family is involved in suppressing the tumor and oncogenic pathways in some cancers. The purpose of this research is to determine the changes in the expression of the Vimentin gene after the replacement of miR-34a, on the other hand the effect of replacing this micro-RNA on cell proliferation is determined.<strong><span dir="RTL"></span></strong><br> <strong>Methods:</strong> After cultivating the TE-8 cell line, miR-34a was replaced in esophageal cancer cells and after miR-34a replacement, <em>Vimentin</em> gene expression was evaluated using qRT-PCR method. Cell proliferation in the TE-8 cell line was evaluated in effective time and dose after the replacement of mir-34a by the MTT method. <span dir="RTL"></span><br> <strong>Results:</strong> Replacing miR-34a in the TE-8 cell line significantly reduced the expression of <em>Vimentin</em> (p<0.0001). Mir-34a could (at an effective dose) within 24 hours significantly reduce cell proliferation (p<0.0001).<br> <strong>Conclusion:</strong> Possibly, the replacement of miR-34a could have a therapeutic role in esophageal cancer.<br> &nbsp;<br> &nbsp; سرطان مری, miRNA-34a, TE-8, ویمنتین Esophageal cancer, miRNA-34a, TE-8, Vimentin 29 38 http://rjms.iums.ac.ir/browse.php?a_code=A-10-3136-2&slc_lang=fa&sid=1 Parvin Moshfegh پروین مشفق 3900319475328460047492 3900319475328460047492 No Islamic Azad University, Bonab, Iran دانشگاه آزاد اسلامی، بناب، ایران Mansoor Aletaha منصور آل طه manal239@yahoo.com 3900319475328460047493 3900319475328460047493 Yes Tabriz University of Medical Sciences, Tabriz, Iran دانشگاه علوم پزشکی تبریز، تبریز، ایران Behzad Baradaran بهزاد برادران 3900319475328460047494 3900319475328460047494 No Tabriz University of Medical Sciences, Tabriz, Iran دانشگاه علوم پزشکی تبریز، تبریز، ایران