Razi Journal of Medical Sciences
مجله علوم پزشکی رازی
RJMS
Medical Sciences
http://rjms.iums.ac.ir
39
journal39
2228-7043
2228-7051
en
jalali
1397
9
1
gregorian
2018
12
1
25
9
online
1
fulltext
fa
تاثیر مصرف مکمل ویتامین D بر سطح پلاسمایی پروتئین ضد پیری کلوتو درسالمندان
The effect of vitamin D supplementation on plasma levels of anti-aging protein in elderly
علوم تغذیه
Nutrition Sciences
پژوهشي
Research
<strong><span style="color:#0070C0;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">زمینه و هدف: </span></span></span></strong><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">درصد افراد سالمند کشور ما رو به رشد است و سالخوردگی با بیماری</span></span></span><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;"></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">های متعدد، ازکارافتادگی و هزینه</span></span></span><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;"></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">های درمانی سنگین همراه است. پروتئین چند عملکردی جدید کلوتو نهتنها سبب کاهش روند پیری می</span></span></span><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;"></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">شود بلکه بر بیماری</span></span></span><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;"></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">های متعدد مرتبط با سنین بالا نیز مؤثر است. این مطالعه با هدف تعیین اثر مکمل ویتامین</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;"> D </span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">بر سطح پلاسمایی کلوتو در سالمندان انجام گردید</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">.</span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;"></span></span></span><br>
<strong><span style="color:#0070C0;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">روش کار:</span></span></span></strong><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> این پژوهش به روش کارآزمایی بالینی تصادفی در سالمندان با سطح پلاسمایی ناکافی ویتامین </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">D</span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">، </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">ng/ml</span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">10-30 به مدت 12 هفته، صورت گرفت. افراد به طور تصادفی به دو گروه دریافتکننده هفته</span></span></span><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;"></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">ای 1 عدد مکمل ویتامین</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;"> D (IU50000)</span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> و دارونما تقسیم شدند. ویتامین</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;"> D </span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">به روش</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;"> Chemiluminescence </span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">و کلوتو به روش</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;"> ELISA </span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">اندازهگیری شدند. آنالیز داده</span></span></span><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;"></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">ها توسط نرمافزار</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">SPSS </span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> نسخه 22 انجام شد و 05/0</span></span></span> <span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">p</span></span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;"><</span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">بهعنوان سطح معنی</span></span></span><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;"></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">داری در نظر گرفته شد</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">.</span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;"></span></span></span><br>
<strong><span style="color:#0070C0;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">یافتهها: </span></span></span></strong><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">میانگین سطح ویتامین </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">D</span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> بعد از مداخله در گروه دارونما و ویتامین </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">D</span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> به ترتیب کاهش و افزایش معنی</span></span></span><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;"></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">داری داشت (به ترتیب 001/0></span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">p</span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> و 001/0></span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">p</span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">) و تغییرات آن بین دو گروه بعد از مداخله نیز دارای تفاوت معنی</span></span></span><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;"></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">داری شد (001/0></span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">p</span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">). میانگین سطح کلوتو پلاسمایی و تغییرات آن بعد از مداخله بین دو گروه نیز تفاوت معنی</span></span></span><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;"></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">داری پیدا کرد (به ترتیب 014/0=</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">p</span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> و 001/0></span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">p</span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">). در گروه ویتامین </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">D</span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> در سطح پروتئین کلوتو بعد از مداخله افزایش معنی</span></span></span><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;"></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">داری مشاهده نشد (164/0=</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">p</span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">).</span></span></span><br>
<strong><span style="color:#0070C0;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">نتیجهگیری:</span></span></span></strong><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> یافته</span></span></span><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;"></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">های پژوهش حاضر نشان داد، مصرف هفته</span></span></span><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;"></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">ای یک عدد مکمل ویتامین </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">D</span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> با دوز </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">IU 50000</span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> در سالمندان، باعث ایجاد تفاوت معنی</span></span></span><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;"></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">داری در تغییرات کلوتو و ویتامین </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">D</span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> بین دو گروه و افزایش معنی</span></span></span><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;"></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">دار سطح ویتامین </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">D</span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> پلاسمایی در گروه ویتامین </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">D</span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> می</span></span></span><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;"></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">شود.</span></span></span> <div></div> <div style="text-align: center;"></div>
<div style="text-align: justify;"><strong>Background:</strong> The percentage of elderly people is growing in our country. Aging is associated with multiple diseases, disabilities and heavy medical expenses. The new multi-functionalProtein, Klotho, not only reduces the aging process but also affects several diseases associated with older ages.The aim of this study was to evaluate the effect of vitamin D supplements on the plasma levels of klotho in the elderly.<br>
<strong>Methods: </strong>This study was conducted in a randomized clinical trial in 81 elderly, with insufficient vitamin D plasma levels (10-30 ng/ml) for 12 weeks.Individuals randomly divided into two groups of vitamin D supplements (50000 IU) and placebo once a week. Vitamin D was measured by chemiluminescence method and klotho by ELISA. Data was analyzed by SPSS (version 22) software at meaningful level of P <0.05.<br>
<strong>Results:</strong> 25(OH)D levels were increased and decreased significantly in vitamin D and placebo group respectively (p<0.001 & p<0.001 respectively), and its changes between the two groups was significantly different at the end of the intervention (p<0.001). The mean klotho protein levels and its changes after the intervention were significantly different between the two groups (p=0.014 & p<0.001 respectively). The mean klotho protein levels in the vitamin D group was not change significantly (p=0.164).<br>
<strong>Conclusion: </strong>The findings of this study showed, weekly intake of 50,000 IU vitamin D for 12 weeks in elderly peoplewith insufficient vitamin D levels, caused significant difference in klotho protein and vitamin D changes between the two groups, and also vitamin D levels in vitamin D group was increased significantly at the end of the intervention.<br>
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ویتامین D, پروتئین کلوتو, سالمند
Vitamin D, Klotho protein, Elderly
84
93
http://rjms.iums.ac.ir/browse.php?a_code=A-10-4051-1&slc_lang=fa&sid=1
Maryam
Jebreil Azimzadeh
مریم
جبرائیل عظیم زاده
azimzadeh_maryam@ymail.com
3900319475328460043196
3900319475328460043196
No
Iran University of Medical Sciences, Tehran, Iran
پردیس بینالملل، دانشگاه علوم پزشکی ایران، تهران، ایران.
farzad
shidfar
فرزاد
شیدفر
shidfar.f@iums.ac.ir
3900319475328460043197
3900319475328460043197
Yes
Iran University of Medical Sciences, Tehran, Iran
دانشگاه علوم پزشکی ایران، تهران، ایران
Shima
Jazayeri
شیما
جزایری
sh_jaz@yahoo.com
3900319475328460043198
3900319475328460043198
No
Iran University of Medical Sciences, Tehran, Iran
دانشگاه علوم پزشکی ایران، تهران، ایران
Fatemeh
Agha Hosseini
فاطمه
آغا حسینی
fatemeh_h@yahoo.com
3900319475328460043199
3900319475328460043199
No
Iran University of Medical Sciences, Tehran, Iran
دانشگاه علوم پزشکی ایران، تهران، ایران