fa تأثیر هشت هفته تمرین مقاومتی بر ActRIIB عضله اسکلتی تند و کند انقباض و سطوح پلاسمایی GDF8، GDF11 و GASP-1 در رت‌های نر پیر فیزیولوژی ورزش Exercise Physiology پژوهشي Research <strong><span style="color:#0070C0;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">زمینه و هدف: </span></span></span></strong><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">تغییر غلظت</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">فاکتورهای</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">رشدی و گیرنده‌های آن می‌تواند سارکوپنیا (آتروفی عضلانی وابسته به سن) را تحت تأثیر قرار دهد. هدف</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">از</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">این</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">مطالعه</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">بررسی تأثیر هشت هفته تمرین مقاومتی بر </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">ActRIIB</span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> در عضله اسکلتی تند و کند انقباض و سطوح پلاسمایی </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">GDF8</span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">، </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">GDF11</span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> و </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">GASP-1</span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> در رت&shy;های نر پیر بود.</span></span></span><br> <strong><span style="color:#0070C0;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">روش کار:</span></span></span></strong> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">مطالعه</span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> حاضر</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">در</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">قالب</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">طرح</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">تجربی</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">دوگروهی</span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> با تعداد 14 سر موش صحرائی نر پیر با محدوده&shy;ی سنی 24 تا 27 ماه و وزن 24</span></span></span><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">&plusmn;</span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">440</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">گرم </span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">انجام</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">شد.</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">پس از تمرینات آشناسازی، رت&shy;ها به‌صورت تصادفی به 2 گروه 7 تایی کنترل و تمرین تقسیم شدند. پروتکل تمرینی شامل 8 هفته صعود از نردبان 1 متری بود. 48 ساعت بعد از آخرین جلسه&shy;ی تمرین مقدار </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">cc</span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">5 خون از بطن چپ به منظور اندازه‌گیری </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">GDF8</span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">، </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">GDF11</span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> و </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">GASP-1</span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> به روش الایزا گرفته شد و بافت&shy;های عضله تاکننده بلند انگشت شست پا (</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">FHL</span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">) و عضله نعلی (</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">SOL</span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">) نیز جهت اندازه‌گیری </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">ActRIIB</span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> به روش </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">IHC</span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> جدا شد. داده‌ها با استفاده از آزمون </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">&nbsp;t</span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> مستقل، در سطح معنی‌داری کمتر از 05/0 تجزیه و تحلیل شدند</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">.</span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;"></span></span></span><br> <strong><span style="color:#0070C0;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">یافته‌ها:</span></span></span></strong><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> مقایسه</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">بین</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">گروهی نشان داد که هشت هفته تمرین مقاومتی تأثیر</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">معنی‌داری بر </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">GDF11</span></span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">(001/0=</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">p</span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">)</span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">، </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">GDF8</span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">/</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">GDF11</span></span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">(001/0=</span></span></span><a name="_Hlk1340690"><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">p</span></span></span></span></a><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">)</span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">، </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">GDF8</span></span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">(0</span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">27</span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">/0=</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">p</span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">)</span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> و </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">ActRII&beta;</span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> عضله کند انقباض </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">Sol</span></span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">(013/0=</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">p</span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">)</span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> دارد؛ اما اختلاف</span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">معنی‌داری در </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">GASP-1</span></span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">(</span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">704</span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">/0=</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">p</span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">) </span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">و </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">ActRII&beta;</span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> عضله تند انقباض </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">FHL</span></span></span></span> <span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">(325/0=</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">p</span></span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">)</span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> مشاهده نشد.</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;"></span></span></span></span><br> <strong><span style="color:#0070C0;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">نتیجه‌گیری:</span></span></span></strong><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;"> به نظر می‌رسد تمرینات مقاومتی می‌تواند با اثرات مطلوب بر فاکتورهای رشدی و گیرنده‌های آن، سارکوپنیا را کاهش دهد. با این حال، باید مطالعات بیشتری در این زمینه انجام شود</span></span></span><span style="color:black;"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">.</span></span></span><br> &nbsp; <strong>Background:</strong> Changing the concentration of growth factors and their receptors can affect sarcopenia (age-related muscular atrophy). The aim of this study was to determine the effects of an 8-week resistance training on ActRII&beta; in fast- and slow-twitch (FT & ST) skeletal muscles and plasma levels of GDF8, GDF11 and GASP1 in old male rats.<br> <strong>Methods:</strong> This study was conducted as a two-group experimental design with 14 old male rats with age range 24 to 27 months and weighing 440&plusmn;24g. After the familiarization training, the rats were randomly divided into 2 groups of 7 control and training. The training protocol was included 8 weeks climbing from a 1 meter ladder. 5cc blood was taken from the left ventricle, 48 hours after the last session, for measurement of GDF8, GDF11 and GASP-1 by ELISA method. The soleus (SOL) muscle (ST) and flexor hallucis longus (FHL) muscle (FT) of rats were taken for measurement of ActRIIB by IHC method. Independent t-test was applied for statistical analysis of the data (p<0.05).<br> <strong>Results:</strong> Comparisons between groups showed that 8-week resistance training have a significant effects on GDF11 (p=0.001), GDF8/GDF11 (p=0.001), GDF8 (p=0.027) and ActRII&beta; in SOL muscle (p=0.013), but no between-groups differences were observed in GASP-1 (p=0.704) and ActRII&beta; in FHL muscle (p=0.325).<br> <strong>Conclusion:</strong> It seems that resistance training can reduce sarcopenia by favorable effects on growth factors and their receptors. However, more studies are needed in this regard. تمرینات قدرتی,ActRIIβ, GDF11, GDF8, GASP-1, پیر Resistance Training, ActRIIβ, GDF11, GDF8, GASP-1, Old 104 115 http://rjms.iums.ac.ir/browse.php?a_code=A-10-4081-1&slc_lang=fa&sid=1 Behnam Masoudian بهنام مسعودیان masoudian_b@yahoo.com 3900319475328460088766 3900319475328460088766 No Shahrekord University دانشگاه شهرکرد Akbar Azamian Jazi اکبر اعظمیان جزی azamianakbar@yahoo.com 3900319475328460088767 3900319475328460088767 Yes Shahrekord University, Shahrekord, Iran دانشگاه شهرکرد، شهرکرد، ایران Mohammad Faramarzi محمد فرامرزی faramerzi@yahoo.com 3900319475328460088768 3900319475328460088768 No Shahrekord University دانشگاه شهرکرد، شهرکرد، ایران Ardeshir Talebi اردشیر طالبی talebi@med.mui.ac.ir 3900319475328460088769 3900319475328460088769 No Isfahan University of Medical Sciences دانشگاه علوم پزشکی اصفهان، اصفهان، ایران