fa اثر دوزهای مختلف عصاره اتانولی اسطوخدوس بر درد حاد در موش صحرایی فارماکولوژی Pharmacology پژوهشي Research <p class="MsoNormal" dir="rtl" style="margin-bottom: 0.0001pt line-height: normal direction: rtl unicode-bidi: embed"><b><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'">زمینه و هدف</span></b><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'">: استفاده</span><span dir="ltr" lang="AR-SA" style="font-size: 9pt font-family: 'Times New Roman', serif"> </span><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'">از</span><span dir="ltr" lang="AR-SA" style="font-size: 9pt font-family: 'Times New Roman', serif"> </span><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'">گیاهان</span><span dir="ltr" lang="AR-SA" style="font-size: 9pt font-family: 'Times New Roman', serif"> </span><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'">دارویی</span><span dir="ltr" lang="AR-SA" style="font-size: 9pt font-family: 'Times New Roman', serif"> </span><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'">جهت</span><span dir="ltr" lang="AR-SA" style="font-size: 9pt font-family: 'Times New Roman', serif"> </span><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'">کاهش</span><span dir="ltr" lang="AR-SA" style="font-size: 9pt font-family: 'Times New Roman', serif"> </span><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'">و</span><span dir="ltr" lang="AR-SA" style="font-size: 9pt font-family: 'Times New Roman', serif"> </span><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'">تسکین</span><span dir="ltr" lang="AR-SA" style="font-size: 9pt font-family: 'Times New Roman', serif"> </span><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'">درد</span><span dir="ltr" lang="AR-SA" style="font-size: 9pt font-family: 'Times New Roman', serif"> </span><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'">اهمیت</span><span dir="ltr" lang="AR-SA" style="font-size: 9pt font-family: 'Times New Roman', serif"> </span><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'">دارد</span><span dir="ltr" style="font-size: 9pt font-family: 'Times New Roman', serif">.</span><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'"> گیاه اسطوخدوس در طب سنتی ایران به‌عنوان یک گیاه دارای خاصیت ضد دردی استفاده می‌شده است. در این مطالعه علاوه بر اندازه‌گیری ترکیبات فنولی، فلاونوئیدی و فلاونولی گیاه، اثر پیش تیمار عصاره اتانولی اسطوخدوس با دوزهای مختلف بر درد حاد در موش‌های صحرایی نر مورد مطالعه قرار گرفت.<!--stripped--><!--stripped--></span></p> <p class="MsoNormal" dir="rtl" style="margin-bottom: 0.0001pt line-height: normal direction: rtl unicode-bidi: embed"><b><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'">روش</span></b><b><span dir="ltr" lang="AR-SA" style="font-size: 9pt font-family: 'Times New Roman', serif"> </span></b><b><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'">کار</span></b><span lang="FA" style="font-size: 10pt font-family: 'B Mitra'">:</span><b><span lang="FA" style="font-size: 10pt font-family: 'B Mitra'"> </span></b><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'">این</span><span dir="ltr" lang="AR-SA" style="font-size: 9pt font-family: 'Times New Roman', serif"> </span><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'">مطالعه</span><span dir="ltr" lang="AR-SA" style="font-size: 9pt font-family: 'Times New Roman', serif"> </span><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'">تجربی</span><span dir="ltr" lang="AR-SA" style="font-size: 9pt font-family: 'Times New Roman', serif"> </span><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'">بر</span><span dir="ltr" lang="AR-SA" style="font-size: 9pt font-family: 'Times New Roman', serif"> </span><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'">روی </span><span dir="ltr" lang="AR-SA" style="font-size: 9pt font-family: 'Times New Roman', serif"> </span><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'">28 سر</span><span dir="ltr" lang="AR-SA" style="font-size: 9pt font-family: 'Times New Roman', serif"> </span><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'">موش</span><span dir="ltr" lang="AR-SA" style="font-size: 9pt font-family: 'Times New Roman', serif"> </span><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'">صحرایی نر نژاد ویستار</span><span dir="ltr" lang="AR-SA" style="font-size: 9pt font-family: 'Times New Roman', serif"> </span><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'">در</span><span dir="ltr" lang="AR-SA" style="font-size: 9pt font-family: 'Times New Roman', serif"> </span><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'">محدوده</span><span dir="ltr" lang="AR-SA" style="font-size: 9pt font-family: 'Times New Roman', serif"> </span><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'">وزنی </span><span dir="ltr" lang="AR-SA" style="font-size: 9pt font-family: 'Times New Roman', serif"> </span><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'">200-250 گرم انجام</span><span dir="ltr" lang="AR-SA" style="font-size: 9pt font-family: 'Times New Roman', serif"> </span><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'">شد. برای</span><span dir="ltr" lang="AR-SA" style="font-size: 9pt font-family: 'Times New Roman', serif"> </span><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'">مطالعه</span><span dir="ltr" lang="AR-SA" style="font-size: 9pt font-family: 'Times New Roman', serif"> </span><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'">درد</span><span dir="ltr" lang="AR-SA" style="font-size: 9pt font-family: 'Times New Roman', serif"> </span><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'">حاد</span><span dir="ltr" lang="AR-SA" style="font-size: 9pt font-family: 'Times New Roman', serif"> </span><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'">از</span><span dir="ltr" lang="AR-SA" style="font-size: 9pt font-family: 'Times New Roman', serif"> </span><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'">آزمون</span><span dir="ltr" lang="AR-SA" style="font-size: 9pt font-family: 'Times New Roman', serif"> </span><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'">صفحه</span><span dir="ltr" lang="AR-SA" style="font-size: 9pt font-family: 'Times New Roman', serif"> </span><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'">داغ</span><span dir="ltr" lang="AR-SA" style="font-size: 9pt font-family: 'Times New Roman', serif"> </span><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'">با</span><span dir="ltr" lang="AR-SA" style="font-size: 9pt font-family: 'Times New Roman', serif"> </span><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'">دمای</span><span dir="ltr" lang="AR-SA" style="font-size: 9pt font-family: 'Times New Roman', serif"> </span><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'">8/52</span><span dir="ltr" lang="AR-SA" style="font-size: 9pt font-family: 'Times New Roman', serif"> </span><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'">درجه</span><span dir="ltr" lang="AR-SA" style="font-size: 9pt font-family: 'Times New Roman', serif"> </span><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'">سانتی‌گراد</span><span dir="ltr" lang="AR-SA" style="font-size: 9pt font-family: 'Times New Roman', serif"> </span><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'">و</span><span dir="ltr" lang="AR-SA" style="font-size: 9pt font-family: 'Times New Roman', serif"> </span><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'">زمان</span><span dir="ltr" lang="AR-SA" style="font-size: 9pt font-family: 'Times New Roman', serif"> </span><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'">قطع</span><span dir="ltr" lang="AR-SA" style="font-size: 9pt font-family: 'Times New Roman', serif"> </span><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'">آزمایش</span><span dir="ltr" lang="AR-SA" style="font-size: 9pt font-family: 'Times New Roman', serif"> </span><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'">60</span><span dir="ltr" lang="AR-SA" style="font-size: 9pt font-family: 'Times New Roman', serif"> </span><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'">ثانیه</span><span dir="ltr" lang="AR-SA" style="font-size: 9pt font-family: 'Times New Roman', serif"> </span><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'">استفاده</span><span dir="ltr" lang="AR-SA" style="font-size: 9pt font-family: 'Times New Roman', serif"> </span><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'">شد</span><span dir="ltr" style="font-size: 9pt font-family: 'Times New Roman', serif">.</span><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'"> در این آزمایش موش‌های صحرایی به‌صورت تصادفی به 4 گروه آزمایشی تقسیم شدند. گروه کنترل آب مقطر دریافت کرد و گروه‌های تیمار عصاره اسطوخدوس (با دوز 100،200 و 400 میلی‌گرم بر کیلوگرم وزن بدن) را 1 ساعت قبل از شروع آزمایش از طریق گاواژ دریافت کردند. داده‌ها از طریق آزمون تجزیه و تحلیل واریانس یک‌طرفه آنالیز شدند.<b><!--stripped--><!--stripped--></b></span></p> <p class="MsoNormal" dir="rtl" style="margin-bottom: 0.0001pt line-height: normal direction: rtl unicode-bidi: embed"><b><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'">یافته‌ها</span></b><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'">:<b> </b>عصاره اسطوخدوس با دوز 400 میلی‌گرم بر کیلوگرم وزن بدن به‌صورت معنی‌داری میزان تأخیر در پاسخ به محرک گرمایی را در زمان‌های 5 و 15 دقیقه بعد از شروع آزمایش افزایش داد.</span></p> <p class="MsoNormal" dir="rtl" style="margin-bottom: 0.0001pt line-height: normal direction: rtl unicode-bidi: embed"><b><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'">نتیجه‌گیری</span></b><span lang="AR-SA" style="font-size: 10pt font-family: 'Times New Roman', serif">:<b> </b></span><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'">اگرچه مطالعات بیشتری در این زمینه مورد نیاز است نتایج نشان می‌دهد که عصاره اسطوخدوس ممکن است در بیمارانی که از درد رنج می‌برند اثرات سودمندی داشته باشد.</span></p> <p dir="ltr" style="text-align: justify"><strong>Background</strong>: The use of medicinal plants is important in reducing pain. Extracts obtained from the leaves of Lavandula officinalis (Lamiaceae) are used in Iranian folk medicine as remedies for anti-nociceptive effects. This study was aimed to investigate the effects of pretreatment with different doses of Lavandula officinalis ethanolic extract on hot plate induced nociception in male Wistar rats.</p> <p dir="ltr" style="text-align: justify"><strong>Methods</strong>: This experimental study was carried out on 28 male Wistar rats weighing 200-250 g. Acute pain was investigated using a hot plate test with set point 52.8 °C and cut off time of 60 seconds. In this experiment rats were divided into 4 groups. Control group received distilled water and three treatment groups received different doses of lavender ethanolic extract (100, 200, 400 mg/kg, respectively) 1 hour prior beginning of experiment via gastric gavage. The data were compared using one way ANOVA (post hoc LSD).</p> <p dir="ltr" style="text-align: justify"><strong>Results</strong>: Lavender extract with 400 mg/kg dose significantly increased latency to respond to heat stimulus 5 and 15 minutes after beginning of the experiment.</p> <p dir="ltr" style="text-align: justify"><strong>Conclusion</strong>: Although further studies are needed, the results indicate that lavender extract might be beneficial in patients suffering pain.</p> گیاه اسطوخدوس, صفحه داغ, درد, موش صحرایی Lavandula officinalis, Hot plate, Pain 115 121 http://rjms.iums.ac.ir/browse.php?a_code=A-10-1-1711&slc_lang=fa&sid=1 زهرا ربیعی 3900319475328460030256 3900319475328460030256 No کز تحقیقات گیاهان دارویی محمدرضا حجتی 3900319475328460030257 3900319475328460030257 No عاطفه مرادی نافچی 3900319475328460030258 3900319475328460030258 No مرکز تحقیقات گیاهان دارویی محمود رفیعیان کوپایی rafieian@yahoo.com 3900319475328460030259 3900319475328460030259 Yes