fa بررسی بیان شاخص‌های بنیادینگی ALDH1 و CD133 در رده‌های سلولی ملانوما A375 و D10 آسیب‌شناسی Pathology پژوهشي Research <p class="MsoNormalCxSpFirst" dir="rtl" style="margin-bottom: 0.0001pt line-height: normal direction: rtl unicode-bidi: embed"><b><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'">زمینه و هدف</span></b><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'">: </span><span lang="FA" style="font-size: 10pt font-family: 'B Mitra'">سلول‌های بنیادی سرطان در تهاجم و متاستاز ملانوما، کشنده‌ترین و تهاجمی‌ترین شکل سرطان پوست، دخیل می‌باشند. در پژوهش حاضر بیان</span><span lang="FA" style="font-size: 10pt font-family: 'B Mitra'"> شاخص‌های کاندید بنیادینگی</span><span dir="ltr"></span><span dir="ltr"></span><span dir="ltr" style="font-size: 9pt font-family: 'Times New Roman', serif"><span dir="ltr"></span><span dir="ltr"></span> CD133 </span><span dir="rtl"></span><span dir="rtl"></span><span lang="FA" style="font-size: 10pt font-family: 'B Mitra'"><span dir="rtl"></span><span dir="rtl"></span> و </span><span dir="ltr" style="font-size: 9pt font-family: 'Times New Roman', serif">ALDH1</span><span dir="rtl"></span><span dir="rtl"></span><span style="font-size: 10pt font-family: 'B Mitra'"><span dir="rtl"></span><span dir="rtl"></span> <span lang="FA">در رده‌های سلولی ملانوما ارزیابی و سپس جداسازی این سلول‌ها بر اساس بیان این شاخص‌ها بررسی شد. <o:p /></span></span></p> <p class="MsoNormalCxSpMiddle" dir="rtl" style="margin-bottom: 0.0001pt line-height: normal direction: rtl unicode-bidi: embed"><b><span lang="FA" style="font-size: 10pt font-family: 'B Mitra'">روش کار</span></b><span lang="FA" style="font-size: 10pt font-family: 'B Mitra'">: در این مطالعه بیان شاخص‌های </span><span dir="ltr" style="font-size: 9pt font-family: 'Times New Roman', serif">ALDH1</span><span dir="rtl"></span><span dir="rtl"></span><span lang="FA" style="font-size: 10pt font-family: 'B Mitra'"><span dir="rtl"></span><span dir="rtl"></span> و </span><span dir="ltr" style="font-size: 9pt font-family: 'Times New Roman', serif">CD133</span><span dir="rtl"></span><span dir="rtl"></span><span style="font-size: 10pt font-family: 'B Mitra'"><span dir="rtl"></span><span dir="rtl"></span> <span lang="FA">در رده‌های سلولی </span></span><span dir="ltr" style="font-size: 9pt font-family: 'Times New Roman', serif">A375</span><span dir="rtl"></span><span dir="rtl"></span><span lang="FA" style="font-size: 10pt font-family: 'B Mitra'"><span dir="rtl"></span><span dir="rtl"></span> و </span><span dir="ltr" style="font-size: 9pt font-family: 'Times New Roman', serif">D10</span><span dir="rtl"></span><span dir="rtl"></span><span style="font-size: 10pt font-family: 'B Mitra'"><span dir="rtl"></span><span dir="rtl"></span> <span lang="FA">با استفاده از روش فلوسایتومتری ارزیابی شد. سپس رده‌ی سلولی و شاخص مناسب انتخاب و سلول‌ها بر اساس بیان و عدم بیان شاخص موردنظر به دو جمعیت مثبت و منفی جداسازی شدند.<o:p /></span></span></p> <p class="MsoNormalCxSpMiddle" dir="rtl" style="margin-bottom: 0.0001pt line-height: normal direction: rtl unicode-bidi: embed"><b><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'">یافته‌ها</span></b><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'">: نتایج این مطالعه نشان داد که شاخص </span><span dir="ltr" style="font-size: 9pt font-family: 'Times New Roman', serif">ALDH1</span><span dir="rtl"></span><span dir="rtl"></span><span lang="FA" style="font-size: 10pt font-family: 'B Mitra'"><span dir="rtl"></span><span dir="rtl"></span> به میزان %88/7</span><span dir="ltr"></span><span dir="ltr"></span><span dir="ltr" style="font-size: 9pt font-family: 'Times New Roman', serif"><span dir="ltr"></span><span dir="ltr"></span>±</span><span dir="rtl"></span><span dir="rtl"></span><span lang="FA" style="font-size: 10pt font-family: 'B Mitra'"><span dir="rtl"></span><span dir="rtl"></span>7/55</span><span lang="FA" style="font-size: 10pt font-family: 'B Mitra'"> </span><span lang="FA" style="font-size: 10pt font-family: 'B Mitra'">و</span><span lang="FA" style="font-size: 10pt font-family: 'B Mitra'"> </span><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'">%43/6</span><span dir="ltr"></span><span dir="ltr"></span><span dir="ltr" style="font-size: 9pt font-family: 'Times New Roman', serif"><span dir="ltr"></span><span dir="ltr"></span>±</span><span dir="rtl"></span><span dir="rtl"></span><span style="font-size: 10pt font-family: 'B Mitra'"><span dir="rtl"></span><span dir="rtl"></span> </span><span lang="FA" style="font-size: 10pt font-family: 'B Mitra'">9/49 به ترتیب در رده‌های سلولی </span><span dir="ltr" style="font-size: 9pt font-family: 'Times New Roman', serif">A375</span><span dir="rtl"></span><span dir="rtl"></span><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'"><span dir="rtl"></span><span dir="rtl"></span> و</span><span dir="ltr" style="font-size: 9pt font-family: 'Times New Roman', serif">D10</span><span dir="ltr" style="font-size: 9pt font-family: 'Times New Roman', serif"> </span><span dir="rtl"></span><span dir="rtl"></span><span lang="FA" style="font-size: 10pt font-family: 'B Mitra'"><span dir="rtl"></span><span dir="rtl"></span> بیان می‌شود، درحالی‌که شاخص </span><span dir="ltr" style="font-size: 9pt font-family: 'Times New Roman', serif">CD133</span><span dir="rtl"></span><span dir="rtl"></span><span lang="FA" style="font-size: 10pt font-family: 'B Mitra'"><span dir="rtl"></span><span dir="rtl"></span> تنها در رده‌ی سلولی </span><span dir="ltr" style="font-size: 9pt font-family: 'Times New Roman', serif">D10</span><span dir="rtl"></span><span dir="rtl"></span><span style="font-size: 10pt font-family: 'B Mitra'"><span dir="rtl"></span><span dir="rtl"></span> <span lang="FA"> به میزان %</span></span><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'">83/2 </span><span dir="ltr"></span><span dir="ltr"></span><span dir="ltr" style="font-size: 9pt font-family: 'Times New Roman', serif"><span dir="ltr"></span><span dir="ltr"></span> ±</span><span dir="rtl"></span><span dir="rtl"></span><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'"><span dir="rtl"></span><span dir="rtl"></span>8/26 بیان شد. جداسازی بر اساس </span><span lang="FA" style="font-size: 10pt font-family: 'B Mitra'">بیان</span><span dir="ltr"></span><span dir="ltr"></span><span lang="FA" dir="ltr" style="font-size: 9pt font-family: 'Times New Roman', serif"><span dir="ltr"></span><span dir="ltr"></span> </span><span dir="ltr" style="font-size: 9pt font-family: 'Times New Roman', serif">ALDH1 </span><span lang="FA" style="font-size: 10pt font-family: 'B Mitra'">در هر دو رده‌ی سلولی و </span><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'">بر اساس </span><span lang="FA" style="font-size: 10pt font-family: 'B Mitra'">بیان </span><span dir="ltr" style="font-size: 9pt font-family: 'Times New Roman', serif">CD133</span><span dir="rtl"></span><span dir="rtl"></span><span lang="FA" style="font-size: 10pt font-family: 'B Mitra'"><span dir="rtl"></span><span dir="rtl"></span> در رده‌ی سلولی</span><span dir="ltr" style="font-size: 9pt font-family: 'Times New Roman', serif">D10</span><span dir="ltr" style="font-size: 9pt font-family: 'Times New Roman', serif"> </span><span dir="rtl"></span><span dir="rtl"></span><span style="font-size: 10pt font-family: 'B Mitra'"><span dir="rtl"></span><span dir="rtl"></span> <span lang="FA">با درصد خلوص بالا ( </span></span><span dir="ltr"></span><span dir="ltr"></span><span dir="ltr" style="font-size: 9pt font-family: 'Times New Roman', serif"><span dir="ltr"></span><span dir="ltr"></span><</span><span dir="rtl"></span><span dir="rtl"></span><span lang="FA" style="font-size: 10pt font-family: 'B Mitra'"><span dir="rtl"></span><span dir="rtl"></span>٩٠%) انجام شد، ولی درصد خلوص جمعیت‌های </span><span dir="ltr" style="font-size: 9pt font-family: 'Times New Roman', serif">ALDH1</span>^{<span dir="ltr" style="font-size: 9pt font-family: 'Times New Roman', serif">+</span>}<span dir="rtl"></span><span dir="rtl"></span><span lang="FA" style="font-size: 10pt font-family: 'B Mitra'"><span dir="rtl"></span><span dir="rtl"></span> و </span><span dir="ltr" style="font-size: 9pt font-family: 'Times New Roman', serif">ALDH1^-</span><span dir="rtl"></span><span dir="rtl"></span><span style="font-size: 10pt font-family: 'B Mitra'"><span dir="rtl"></span><span dir="rtl"></span> <span lang="FA">جداسازی شده بسیار پایین بود.  </span></span><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'"><o:p /></span></p> <p class="MsoNormalCxSpMiddle" dir="rtl" style="margin-bottom: 0.0001pt line-height: normal direction: rtl unicode-bidi: embed"><b><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'">نتیجه‌گیری</span></b><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'">: یافته‌های این پژوهش</span><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'"> </span><span lang="FA" style="font-size: 10pt font-family: 'B Mitra'">نشان داد که جداسازی سلول‌ها بر اساس شاخص بنیادینگی </span><span dir="ltr" style="font-size: 9pt font-family: 'Times New Roman', serif">CD133</span><span dir="rtl"></span><span dir="rtl"></span><span style="font-size: 10pt font-family: 'B Mitra'"><span dir="rtl"></span><span dir="rtl"></span> </span><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra'">در رده‌ی سلولی </span><span dir="ltr" style="font-size: 9pt font-family: 'Times New Roman', serif">D10</span><span dir="rtl"></span><span dir="rtl"></span><span style="font-size: 10pt font-family: 'B Mitra'"><span dir="rtl"></span><span dir="rtl"></span> <span lang="FA">به‌خوبی انجام‌شده و این شاخص می‌تواند به‌عنوان کاندید مناسب برای شناسایی سلول‌های بنیادی سرطان  در این رده سلولی در نظر گرفته شود. </span></span><span dir="ltr" style="font-size: 9pt font-family: 'Times New Roman', serif"><o:p /></span></p> <p class="MsoNormal" style="margin-bottom: 0.0001pt line-height: normal"><b><span style="font-size: 12pt font-family: 'Times New Roman', serif">Background</span></b><span style="font-size: 12pt font-family: 'Times New Roman', serif">: The cancer stem cells (CSCs) are involved in invasion and metastasis of melanoma, the most lethal and aggressive form of skin cancer. In the present study, the expression of putative stem cell markers CD133 and ALDH1 were evaluated in melanoma cell lines and then the cells sorted based on the expression of these markers.<o:p /></span></p> <p class="MsoNormal" style="margin-bottom: 0.0001pt line-height: normal"><b><span style="font-size: 12pt font-family: 'Times New Roman', serif">Methods</span></b><span style="font-size: 12pt font-family: 'Times New Roman', serif">: In the present study expression of CD133 and ALDH1 was evaluated in A375 and D10 cell lines using flow cytometry. Then, selected cell line was sorted up on the expression of selected stem cell marker into positive and negative populations.<o:p /></span></p> <p class="MsoNormal" style="margin-bottom: 0.0001pt line-height: normal"><b><span style="font-size: 12pt font-family: 'Times New Roman', serif">Results</span></b><span style="font-size: 12pt font-family: 'Times New Roman', serif">: Our results indicated that ALDH1 marker expressed in 55.71 ± 7.88% and 49.88 ± 6.43% in A375 and D10 cell lines, respectively, while the CD133 only expressed in D10 cells in 26.76 ± 2.83%. Cell sorting was performed according to ALDH1 expression in both cell lines and CD133 expression only in D10 cells. The purity of isolated cells was high according to CD133 expression (90% <), whereas the purity of ALDH1+ and ALDH1- populations was very low.<o:p /></span></p> <p class="MsoNormal" style="margin-bottom: 0.0001pt line-height: normal"><b><span style="font-size: 12pt font-family: 'Times New Roman', serif">Conclusion</span></b><span style="font-size: 12pt font-family: 'Times New Roman', serif">: Findings of this study showed that cell sorting based on stem cell marker CD133 was suitable in D10 cells, therefore this marker could be a reliable marker for isolating of CSCs in this cell line.<o:p /></span></p> <p class="MsoNormal" style="margin-bottom: 0.0001pt line-height: normal"><span lang="FA" dir="rtl" style="font-size: 12pt font-family: 'Times New Roman', serif"><!--stripped--> <!--stripped--></span></p> ملانوما, شاخص‌های بنیادینگی, CD133,ALDH1 , A375, D10 Melanoma, Stem cell marker, CD133, ALDH1, A375, D10 8 15 http://rjms.iums.ac.ir/browse.php?a_code=A-10-1-1676&slc_lang=fa&sid=1 مهرداد نصرالله زاده ثابت 3900319475328460030010 3900319475328460030010 No راحله رودی 3900319475328460030011 3900319475328460030011 No مرضیه ابراهیمی 3900319475328460030012 3900319475328460030012 No پژوهشگاه رویان علی صمدی کوچکسرایی 3900319475328460030013 3900319475328460030013 No مطهره رجبی فومشی 3900319475328460030014 3900319475328460030014 No پژوهشگاه رویان زهرا مجد madjd.z@iums.ac.ir 3900319475328460030015 3900319475328460030015 Yes