fa تغییرات سطوح GDNF ساقه مغز موش های پارکینسونی به دنبال مصرف عصاره گل ازگیل ژاپنی و 12 هفته تمرین اختیاری فیزیولوژی ورزش Exercise Physiology پژوهشي Research <font face="Times New Roman" size="3">&nbsp;</font> <p class="MsoNormal" dir="rtl" style="margin: 0cm 0cm 0pt text-align: justify line-height: normal unicode-bidi: embed direction: rtl text-justify: kashida text-kashida: 0%"><b><span 10pt="" 9.0pt="" b="" font-size:="" lang="AR-SA" mso-ansi-font-size:="" mso-ascii-font-family:="" mso-hansi-font-family:="" new="" span="" style="font-family: " times="">: </span><span 10pt="" 9.0pt="" 9pt="" b="" dir="ltr" fa="" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ascii-font-family:="" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-bidi-language:="" mso-hansi-font-family:="" new="" span="" style="font-family: " times="">گل</span><span dir="ltr"></span><span dir="ltr"></span><span 9pt="" b="" dir="ltr" fa="" font-size:="" lang="FA" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-bidi-language:="" new="" style="font-family: " times=""><span dir="ltr"></span><span dir="ltr"></span> </span><span 10pt="" 9.0pt="" 9pt="" b="" dir="ltr" fa="" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ascii-font-family:="" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-bidi-language:="" mso-hansi-font-family:="" new="" span="" style="font-family: " times="">ژاپنی</span><span dir="ltr"></span><span dir="ltr"></span><span 9pt="" b="" dir="ltr" fa="" font-size:="" lang="FA" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-bidi-language:="" new="" style="font-family: " times=""><span dir="ltr"></span><span dir="ltr"></span> </span><span 10pt="" 9.0pt="" 9pt="" b="" dir="ltr" fa="" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ascii-font-family:="" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-bidi-language:="" mso-hansi-font-family:="" new="" span="" style="font-family: " times="">های</span><span dir="ltr"></span><span dir="ltr"></span><span 9pt="" b="" dir="ltr" fa="" font-size:="" lang="FA" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-bidi-language:="" new="" style="font-family: " times=""><span dir="ltr"></span><span dir="ltr"></span> </span><span 10pt="" 9.0pt="" 9pt="" b="" dir="ltr" fa="" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ascii-font-family:="" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-bidi-language:="" mso-hansi-font-family:="" new="" span="" style="font-family: " times=""><span dir="rtl"></span><span dir="rtl"></span>12هفته</span><span dir="ltr"></span><span dir="ltr"></span><span 9pt="" b="" dir="ltr" fa="" font-size:="" lang="FA" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-bidi-language:="" new="" style="font-family: " times=""><span dir="ltr"></span><span dir="ltr"></span> </span><span 10pt="" 9.0pt="" 9pt="" b="" dir="ltr" fa="" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ascii-font-family:="" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-bidi-language:="" mso-hansi-font-family:="" new="" span="" style="font-family: " times="">اختیاری روی چرخ</span><span dir="ltr"></span><span dir="ltr"></span><span 9pt="" b="" dir="ltr" fa="" font-size:="" lang="FA" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-bidi-language:="" new="" style="font-family: " times=""><span dir="ltr"></span><span dir="ltr"></span> </span><span 10pt="" 9.0pt="" b="" class="MsoNormal" dir="rtl" fa="" face="Times New Roman" font="" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ascii-font-family:="" mso-bidi-language:="" mso-hansi-font-family:="" new="" p="" size="3" span="" style="font-family: " times="">روش کار</span></b><span 10pt="" 9.0pt="" 9pt="" :="" b="" dir="ltr" fa="" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ascii-font-family:="" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-bidi-language:="" mso-hansi-font-family:="" new="" span="" style="font-family: " times="">های صحرایی به 6 گروه: کنترل (کنترل سالم)، کنترل پارکینسونی، تمرین، تمرین-سم، عصاره-سم، و تمرین-عصاره-سم تقسیم شدند. گروه تمرین به مدت 12 هفته تمرین انجام داد. گروه تمرین-سم 12 هفته تمرین انجام داد، سپس در معرض سم نورونی قرار گرفت. گروه تمرین- عصاره به مدت دوازده هفته تمرین کرد و هر هفته سه بار عصاره را به صورت صفاقی و به میزان 200 میلی گرم به ازای هر کیلوگرم دریافت کرد. تخریب ساقه مغز با تزریق محلول 6-هیدروکسی دوپامین با استریوتاکسی به داخل بطن مغز صورت گرفت. داده&shy;ها به روش </span><span 9pt="" b="" dir="ltr" fa="" font-size:="" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-bidi-language:="" new="" style="font-family: " times="">One way ANOVA</span><span dir="rtl"></span><span dir="rtl"></span><span 10pt="" 9.0pt="" 9pt="" b="" dir="ltr" fa="" face="B Mitra" font="" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ascii-font-family:="" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-bidi-language:="" mso-hansi-font-family:="" new="" size="2" span="" style="font-family: " times="">تعقیبی</span><span dir="ltr"></span><span dir="ltr"></span><span 9pt="" b="" dir="ltr" fa="" font-size:="" lang="FA" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-bidi-language:="" new="" style="font-family: " times=""><span dir="ltr"></span><span dir="ltr"></span> </span><span 9pt="" b="" dir="ltr" fa="" font-size:="" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-bidi-language:="" new="" style="font-family: " times="">tukey</span><span dir="rtl"></span><span dir="rtl"></span><span 10pt="" 9.0pt="" 9pt="" b="" dir="ltr" fa="" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ascii-font-family:="" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-bidi-language:="" mso-hansi-font-family:="" new="" span="" style="font-family: " times="">و تحلیل</span><span dir="ltr"></span><span dir="ltr"></span><span 9pt="" b="" dir="ltr" fa="" font-size:="" lang="FA" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-bidi-language:="" new="" style="font-family: " times=""><span dir="ltr"></span><span dir="ltr"></span> </span><span 10pt="" 9.0pt="" b="" class="MsoNormal" dir="rtl" fa="" face="Times New Roman" font="" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ascii-font-family:="" mso-bidi-language:="" mso-hansi-font-family:="" new="" p="" size="3" span="" style="font-family: " times="">یافته ها</span><span 10pt="" 9.0pt="" 9pt="" :="" b="" dir="ltr" fa="" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ascii-font-family:="" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-bidi-language:="" mso-hansi-font-family:="" new="" p="</span" span="" style="font-family: " times=""><span dir="rtl"></span><span dir="rtl"></span>). همچنین مقدار این پروتئین در گروه&shy;تمرین پارکینسون با گروه کنترل پارکینسون (015/0</span><span 9pt="" b="" dir="ltr" fa="" font-size:="" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-bidi-language:="" new="" style="font-family: " times="">p=</span><span dir="rtl"></span><span dir="rtl"></span><span .="" 10pt="" 9.0pt="" 9pt="" b="" dir="ltr" fa="" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ascii-font-family:="" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-bidi-language:="" mso-hansi-font-family:="" new="" p="</span" span="" style="font-family: " times=""><span dir="rtl"></span><span dir="rtl"></span>). بین سطوح این فاکتور در گروه</span><span 14pt="" b="" font-size:="" lang="FA" mso-bidi-language:="" style="color: black font-family: ">&nbsp;</span><span 10pt="" 9.0pt="" 9pt="" b="" dir="ltr" fa="" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ascii-font-family:="" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-bidi-language:="" mso-hansi-font-family:="" new="" p="</span" span="" style="font-family: " times=""><span dir="rtl"></span><span dir="rtl"></span>). سطوح این نورتروفین ساقه مغز در گروه عصاره-پارکینسون با گروه کنترل پارکینسون (191/0</span><span 9pt="" b="" dir="ltr" fa="" font-size:="" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-bidi-language:="" new="" style="font-family: " times="">p=</span><span dir="rtl"></span><span dir="rtl"></span><span 10pt="" 9.0pt="" 9pt="" b="" dir="ltr" fa="" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ascii-font-family:="" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-bidi-language:="" mso-hansi-font-family:="" new="" p="</span" span="" style="font-family: " times=""><span dir="rtl"></span><span dir="rtl"></span>) تفاوت معناداری نداشت.</span></p> <font face="Times New Roman" size="3">&nbsp;</font> <p class="MsoNormal" dir="rtl" style="margin: 0cm 0cm 0pt text-align: justify line-height: normal unicode-bidi: embed direction: rtl text-justify: kashida text-kashida: 0%"><b><span 10pt="" 9.0pt="" b="" fa="" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ascii-font-family:="" mso-bidi-language:="" mso-hansi-font-family:="" new="" span="" style="font-family: " times="">: پیش درمان با استفاده از ورزش به تنهایی و انجام ورزش اختیاری همراه با مصرف عصاره توانست از کاهش مقدار فاکتور نوروتروفیک مشتق از گلیال در برابر سم عصبی 6 هیدروکسی دوپامین جلوگیری کند. در نتیجه این پروتکل دارای اثر حفاظتی می باشد.</span></b></p> <b><font face="Times New Roman" size="3">&nbsp;</font></b> <font face="Times New Roman" size="3">&nbsp;</font> <p class="MsoNormal" style="margin: 0cm 0cm 0pt text-align: justify line-height: normal text-justify: kashida text-kashida: 0%"><b><span 12pt="" font-size:="" mso-bidi-language:="" new="" style="font-family: " times="">Background</span></b><span 12pt="" font-size:="" mso-bidi-language:="" new="" style="font-family: " times="">: We investigated the effects of 12 weeks of voluntary exercise on a running wheel with extraction of flowers Eriobotrya japonica on GDNF in the brain stem induced by 6-hydroxy dopamine.</span></p> <font face="Times New Roman" size="3">&nbsp;</font> <p class="MsoNormal" style="margin: 0cm 0cm 0pt text-align: justify line-height: normal text-justify: kashida text-kashida: 0%"><b><span 12pt="" font-size:="" mso-bidi-language:="" new="" style="font-family: " times="">Methods</span></b><span 12pt="" font-size:="" mso-bidi-language:="" new="" style="font-family: " times="">: In this study, 43 rats were divided into six groups: healthy control, parkinsonian control, training group, parkinsonian training, extract parkinson, training-extract parkinsonian. Training-extract group were housed in individual cages and attached to running wheels during the study period they received 200 mg/kg extract intraperitoneally three times per week. To induce Parkinson, 6-hydroxy dopamine (6-OHDA) (dissolved in saline) was administered intracerebrovenricular (ICV) by a stereotaxic apparatus. GDNF levels in the brain stem were measured by ELISA. Data was analyzed using one-way analysis of variance (ANOVA) and tukey post-hoc test.</span></p> <font face="Times New Roman" size="3">&nbsp;</font> <p class="MsoNormal" style="background: white margin: 0cm 0cm 0pt text-align: justify text-justify: inter-ideograph"><font style="background-color: rgb(255, 255, 255)"><b><span 12pt="" font-size:="" mso-bidi-language:="" new="" style="line-height: 115% font-family: " times="">Results</span></b><span 12pt="" font-size:="" mso-bidi-language:="" new="" style="line-height: 115% font-family: " times="">: There are significant differences in the level of GDNF between training group and parkinsonian control (p=0.015) between parkinsonian training group and parkinsonian control group there was significant difference (p=0.015). GDNF level between training group and parkinsonian training with parkinsonian control was not significant (0. 87, 0.095). There was significant difference between control and parkinsonian control group (p=0.004). Difference between extract-parkinson group and parkinsonian control was not significant (p=0.191). GDNF level difference between extract-training and parkinsonian control was significant (p=0.008). Brainstem GDNF level in extract-parkinson and parkinsonian control (p=0.191) and extract-parkinson and control (P=0.164) was not significant.</span></font></p> <font face="Times New Roman" size="3">&nbsp;</font> <p class="MsoNormal" style="margin: 0cm 0cm 0pt text-align: justify line-height: normal text-justify: kashida text-kashida: 0%"><b><span 12pt="" font-size:="" mso-bidi-language:="" new="" style="font-family: " times="">Conclusion</span></b><span 12pt="" font-size:="" mso-bidi-language:="" new="" style="font-family: " times="">: Pre-treatment with exercise alone and exercise with extraction of Eriobotrya japonica could prevent the decrease of GDNF level in brainstem against neurotoxic 6-OHDA.</span></p> <font face="Times New Roman" size="3">&nbsp;</font> <p class="MsoNormal" style="margin: 0cm 0cm 0pt text-align: justify line-height: normal text-justify: kashida text-kashida: 0%"><span 12pt="" font-size:="" mso-bidi-language:="" new="" style="font-family: " times=""><font size="3">&nbsp;</font></span></p> <font face="Times New Roman" size="3">&nbsp;</font> فاکتور نوروتروفیک مشتق از گلیال, ساقه مغز, عصاره گل ازگیل ژاپنی, تمرین اختیاری, 6-هیدروکسی دوپامین GDNF, Brainstem, Eriobotrya japonica, Voluntary exercise, 6-hydroxydopamine 1 7 http://rjms.iums.ac.ir/browse.php?a_code=A-10-1-1632&slc_lang=fa&sid=1 Raziyeh Mohammadi راضیه محمدی 3900319475328460043509 3900319475328460043509 Yes University of Mazandaran, Babolsar, Iran ، دانشگاه مازندران، بابلسر، مازندران Zia Fallahmohammadi ضیاء فلاح محمدی 3900319475328460043510 3900319475328460043510 No University of Mazandaran, Babolsar, Iran ، دانشگاه مازندران، بابلسر، مازندران Khadijeh Aghajani خدیجه آقاجانی 3900319475328460043511 3900319475328460043511 No Branch of sari, Sari واحد علوم تحقیقات ساری، ساری، ایران