fa ﺗﺃثیر تمرین استقامتی و مصرف مکمل امگا-۳ بر عامل نوروتروفیک مشتق از مغز (BDNF) در هیپوکمپ موش‌های صحرایی نر بالغ فیزیولوژی Physiology پژوهشي Research <font face="Times New Roman" size="3"> </font><p class="MsoNormal" style="margin: 0in 0in 0pt text-align: justify line-height: normal unicode-bidi: embed direction: rtl text-justify: kashida text-kashida: 0%" dir="rtl"><b><span lang="AR-SA" style="font-family: "B Mitra" font-size: 10pt mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 9.0pt">زمینه و هدف</span></b><span lang="AR-SA" style="font-family: "B Mitra" font-size: 10pt mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 9.0pt">: هدف از پژوهش حاضر بررسی ﺗﺃثیر 8 هفته تمرین استقامتی و مصرف مکمل امگا-3 بر مقادیر عامل نوروتروفیک مشتق از مغز (</span><span style="font-family: "Times New Roman","serif" font-size: 9pt mso-bidi-font-family: "B Mitra" mso-bidi-font-size: 10.0pt" dir="ltr">BDNF</span><span dir="rtl"></span><span lang="AR-SA" style="font-family: "B Mitra" font-size: 10pt mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 9.0pt"><span dir="rtl"></span>) در هیپوکمپ موش­های صحرایی نر بالغ بود.<o:p /></span></p><font face="Times New Roman" size="3"> </font><p class="MsoNormal" style="margin: 0in 0in 0pt text-align: justify line-height: normal unicode-bidi: embed direction: rtl text-justify: kashida text-kashida: 0%" dir="rtl"><b><span lang="AR-SA" style="font-family: "B Mitra" font-size: 10pt mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 9.0pt">روش کار</span></b><span lang="AR-SA" style="font-family: "B Mitra" font-size: 10pt mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 9.0pt">: در این پژوهش تجربی 28 سر موش صحرایی (</span><span style="font-family: "Times New Roman","serif" font-size: 9pt mso-bidi-font-family: "B Mitra" mso-bidi-font-size: 10.0pt" dir="ltr">rat</span><span dir="rtl"></span><span lang="AR-SA" style="font-family: "B Mitra" font-size: 10pt mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 9.0pt"><span dir="rtl"></span>) نر نژاد ویستار (با سن 8 هفته­ و میانگین وزن 10±170 گرم)، در 4 گروه مساوی 7 تایی کنترل، تمرین، مکمل و تمرین- مکمل به طور تصادفی تقسیم شدند. گروه تمرین، تحت تمرین استقامتی روی نوارگردان به مدت 8 هفته قرار گرفتند. گروه مکمل، روزانه (100 میلی­گرم/کیلوگرم وزن بدن) مکمل امگا-3 به صورت خوراکی به روش گاواژ دریافت کردند. گروه تمرین- مکمل نیز تحت ترکیبی از همان برنامه تمرینی به همراه با مصرف رژیم مکمل قرار گرفتند. مقادیر </span><span style="font-family: "Times New Roman","serif" font-size: 9pt mso-bidi-font-family: "B Mitra" mso-bidi-font-size: 10.0pt" dir="ltr">BDNF</span><span dir="rtl"></span><span style="font-family: "B Mitra" font-size: 10pt mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 9.0pt"><span dir="rtl"></span> <span lang="AR-SA"><font face="B Mitra" size="2">با روش الایزا سنجیده شد و داده</font></span></span><span style="font-family: "Times New Roman","serif" font-size: 9pt mso-bidi-font-family: "B Mitra" mso-bidi-font-size: 10.0pt" dir="ltr">­</span><span lang="AR-SA" style="font-family: "B Mitra" font-size: 10pt mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 9.0pt">ها با استفاده از روش آماری آنالیز واریانس یک راهه با 05/0</span><span style="font-family: "Times New Roman","serif" font-size: 9pt mso-bidi-font-family: "B Mitra" mso-bidi-font-size: 10.0pt" dir="ltr">p <</span><span dir="rtl"></span><span lang="AR-SA" style="font-family: "B Mitra" font-size: 10pt mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 9.0pt"><span dir="rtl"></span> تحلیل شدند.</span><span lang="FA" style="font-family: "B Mitra" font-size: 13pt mso-bidi-language: FA"><o:p /></span></p><font face="Times New Roman" size="3"> </font><p class="MsoNormal" style="margin: 0in 0in 0pt text-align: justify line-height: normal unicode-bidi: embed direction: rtl text-justify: kashida text-kashida: 0%" dir="rtl"><b><span lang="AR-SA" style="font-family: "B Mitra" font-size: 10pt mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 9.0pt">یافته ها:</span></b><span lang="AR-SA" style="font-family: "B Mitra" font-size: 13pt mso-bidi-language: FA"> </span><span lang="AR-SA" style="font-family: "B Mitra" font-size: 10pt mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 9.0pt">یافته­های پژوهش حاضر نشان داد، مقادیر </span><span style="font-family: "Times New Roman","serif" font-size: 9pt mso-bidi-font-family: "B Mitra" mso-bidi-font-size: 10.0pt" dir="ltr">BDNF</span><span dir="rtl"></span><span style="font-family: "B Mitra" font-size: 10pt mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 9.0pt"><span dir="rtl"></span> <span lang="AR-SA"><font face="B Mitra" size="2">هیپوکمپ پس از 8 هفته، در گروه تمرین و گروه مکمل نسبت به گروه کنترل افزایش معنی­داری نداشتند (447/0=</font></span></span><span style="font-family: "Times New Roman","serif" font-size: 9pt mso-bidi-font-family: "B Mitra" mso-bidi-font-size: 10.0pt" dir="ltr">p</span><span dir="rtl"></span><span style="font-family: "B Mitra" font-size: 10pt mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 9.0pt"><span dir="rtl"></span> <span lang="AR-SA"><font face="B Mitra" size="2">و 054/0=</font></span></span><span style="font-family: "Times New Roman","serif" font-size: 9pt mso-bidi-font-family: "B Mitra" mso-bidi-font-size: 10.0pt" dir="ltr">p</span><span dir="rtl"></span><span lang="AR-SA" style="font-family: "B Mitra" font-size: 10pt mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 9.0pt"><span dir="rtl"></span>). بعلاوه مقادیر </span><span style="font-family: "Times New Roman","serif" font-size: 9pt mso-bidi-font-family: "B Mitra" mso-bidi-font-size: 10.0pt" dir="ltr">BDNF</span><span dir="rtl"></span><span style="font-family: "B Mitra" font-size: 10pt mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 9.0pt"><span dir="rtl"></span> <span lang="AR-SA"><font face="B Mitra" size="2">در گروه تمرین- مکمل، در مقایسه با گروه کنترل و گروه تمرین افزایش معنی­داری داشت (003/0=</font></span></span><span style="font-family: "Times New Roman","serif" font-size: 9pt mso-bidi-font-family: "B Mitra" mso-bidi-font-size: 10.0pt" dir="ltr">p</span><span dir="rtl"></span><span style="font-family: "B Mitra" font-size: 10pt mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 9.0pt"><span dir="rtl"></span> <span lang="AR-SA"><font face="B Mitra" size="2">و 021/0=</font></span></span><span style="font-family: "Times New Roman","serif" font-size: 9pt mso-bidi-font-family: "B Mitra" mso-bidi-font-size: 10.0pt" dir="ltr">p</span><span dir="rtl"></span><span lang="AR-SA" style="font-family: "B Mitra" font-size: 10pt mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 9.0pt"><span dir="rtl"></span>) و در مقایسه با گروه مکمل این افزایش معنی­دار نبود (237/0=</span><span style="font-family: "Times New Roman","serif" font-size: 9pt mso-bidi-font-family: "B Mitra" mso-bidi-font-size: 10.0pt" dir="ltr">p</span><span dir="rtl"></span><span lang="AR-SA" style="font-family: "B Mitra" font-size: 10pt mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 9.0pt"><span dir="rtl"></span>).<o:p /></span></p><font face="Times New Roman" size="3"> </font><p class="MsoNormal" style="margin: 0in 0in 0pt text-align: justify line-height: normal unicode-bidi: embed direction: rtl text-justify: kashida text-kashida: 0%" dir="rtl"><b><span lang="AR-SA" style="font-family: "B Mitra" font-size: 10pt mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 9.0pt">نتیجه</span></b><b><span style="font-family: "Times New Roman","serif" font-size: 9pt mso-bidi-font-size: 10.0pt" dir="ltr">‌</span></b><b><span lang="AR-SA" style="font-family: "B Mitra" font-size: 10pt mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 9.0pt">گیری</span></b><span lang="AR-SA" style="font-family: "B Mitra" font-size: 10pt mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 9.0pt">: با توجه به پارامترهای حاصل از مطالعه به نظر می­رسد، فعالیت ورزشی استقامتی همراه با مصرف مکمل امگا-3 می­تواند موجب افزایش بیشتر مقادیر </span><span style="font-family: "Times New Roman","serif" font-size: 9pt mso-bidi-font-family: "B Mitra" mso-bidi-font-size: 10.0pt" dir="ltr">BDNF</span><span dir="rtl"></span><span style="font-family: "B Mitra" font-size: 10pt mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 9.0pt"><span dir="rtl"></span> <span lang="AR-SA"><font size="2"><font face="B Mitra">هیپوکمپ موش­های صحرایی نر بالغ شود.<o:p /></font></font></span></span></p><font face="Times New Roman" size="3"> </font> <font face="Times New Roman" size="3"> </font><p class="MsoNormal" style="margin: 0in 0in 0pt text-align: justify line-height: normal text-justify: inter-ideograph"><b><span style="font-family: "Times New Roman","serif" font-size: 12pt mso-bidi-language: FA">Background: </span></b><span style="font-family: "Times New Roman","serif" font-size: 12pt">This study was to examine the effects of endurance training and omega-3 supplementation in brain-derived neurotrophic factor (BDNF) in the male adult rat hippocampus. </span><span style="font-size: 12pt mso-ascii-font-family: Calibri mso-fareast-font-family: Calibri mso-hansi-font-family: Calibri mso-bidi-font-family: "B Mitra""><o:p /></span></p><font face="Times New Roman" size="3"> </font><p class="MsoNormal" style="margin: 0in 0in 0pt text-align: justify line-height: normal text-justify: inter-ideograph"><b><span style="font-family: "Times New Roman","serif" font-size: 12pt">Methods</span></b><span style="font-family: "Times New Roman","serif" font-size: 12pt">: Twenty eight Wistar rats were divided into four equal groups: control, exercise, diet, and exercise with diet Animals in the exercise group received 8 weeks of endurance training and animals in the diet group were treated with 100/mg/kg/day of omega-3 supplementation via oral gavage for 8weeks. The exercise with diet group received a combination of exercise and also supplementation. Hippocampal BDNF protein was assessed using commercial ELISA methods andthe data were analyzed by one-way ANOVA (p<0.05).<span lang="AR-SA" dir="rtl"><o:p /></span></span></p><font face="Times New Roman" size="3"> </font><p class="MsoNormal" style="margin: 0in 0in 0pt text-align: justify line-height: normal text-justify: inter-ideograph tab-stops: right 7.3pt center 265.9pt"><b><span style="font-family: "Times New Roman","serif" font-size: 12pt">Results:</span></b><span style="font-size: 12pt mso-ascii-font-family: Calibri mso-fareast-font-family: "Times New Roman" mso-hansi-font-family: Calibri mso-bidi-font-family: "B Mitra""><font face="Calibri"> </font></span><span style="font-family: "Times New Roman","serif" font-size: 12pt">The results showed that BDNF level showed no significance increase in the exercise and diet alone groups. , Moreover, the diet and exercise group revealed a significant increase in BDNF level in comparison with the control and the exercise groups but it was not significant compared with the diet group.<o:p /></span></p><font face="Times New Roman" size="3"> </font><p class="MsoNormal" style="margin: 0in 0in 0pt text-align: justify line-height: normal text-justify: inter-ideograph tab-stops: right 7.3pt center 265.9pt"><b><span style="font-family: "Times New Roman","serif" font-size: 12pt">Conclusions: </span></b><span style="font-family: "Times New Roman","serif" font-size: 12pt">The results of the present study suggest that concurrent administration of omega-3 diet and endurance training has an additive effect in the BDNF level in adult male rat hippocampus.<o:p /></span></p><font face="Times New Roman" size="3"> </font> عامل نوروتروفیک مشتق از مغز (BDNF)، امگا-3، هیپوکمپ، فعالیت ورزشی استقامتی. Brain-derived neurotrophic factor (BDNF), Omega-3, Hippocampus, Physical endurance. 50 57 http://rjms.iums.ac.ir/browse.php?a_code=A-10-1-1448&slc_lang=fa&sid=1 Elham Vosadi الهام وسدی 3900319475328460023988 3900319475328460023988 Yes Tehran University دانشگاه تهران Ali Asghar Ravasi علی اصغر رواسی 3900319475328460023989 3900319475328460023989 No Tehran University دانشگاه تهران Siroos Choobine سیروس چوبینه 3900319475328460023990 3900319475328460023990 No Tehran University دانشگاه تهران Hamed Barzegar حامد برزگر 3900319475328460023991 3900319475328460023991 No Tehran University دانشگاه تهران Mahboobe Borjianfard محبوبه برجیان فرد 3900319475328460023992 3900319475328460023992 No Tehran University دانشگاه تهران