fa مطالعه اثر ملاتونین اگزوژن بر باروری اسپرم در مدل الیگواسپرمی القاء شده با بوسولفان در موش‌های صحرایی پینئالکتومی شده علوم تشریحی Anatomy پژوهشي Research <p align="right"> <font face="Times New Roman" size="3"> </font></p><p class="MsoNormal" style="margin: 0in 0in 0pt text-align: justify line-height: normal unicode-bidi: embed direction: rtl text-justify: kashida text-kashida: 0%" dir="rtl"><b><span lang="AR-SA" style="font-family: "B Mitra" font-size: 10pt mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 9.0pt">زمینه و هدف</span></b><span lang="AR-SA" style="font-family: "B Mitra" font-size: 10pt mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 9.0pt">: بوسولفان از داروهای اختصاصی در درمان سرطان می‌باشد که عوارض جانبی زیادی دارد. از میان آن‌ها می‌توان به آسیب بافت بیضه و اختلالات باروری اشاره کرد. برای کاهش عوارض جانبی این داروها هنوز روش درمانی مطمئنی پیدا نشده است. با توجه به نقش حفاظتی ملاتونین، هدف از مطالعه حاضر بررسی اثرات ملاتونین اگزوژن در بهبود الیگواسپرمی ناشی از درمان با بوسولفان در</span><span dir="ltr"></span><span lang="AR-SA" style="font-family: "Times New Roman","serif" font-size: 9pt mso-bidi-font-family: "B Mitra" mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span> </span><span lang="AR-SA" style="font-family: "B Mitra" font-size: 10pt mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 9.0pt">بافت بیضه و تاثیر آن بر باروری است.<o:p /></span></p><p align="right"><font face="Times New Roman" size="3"> </font></p><p class="MsoNormal" style="margin: 0in -0.05pt 0pt 0in text-align: justify line-height: normal unicode-bidi: embed direction: rtl text-justify: inter-ideograph tab-stops: 35.4pt right 2.25in 189.0pt 207.0pt" dir="rtl"><b><span lang="AR-SA" style="font-family: "B Mitra" font-size: 10pt mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 9.0pt">روش کار</span></b><span lang="AR-SA" style="font-family: "B Mitra" font-size: 10pt mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 9.0pt">: تحقیق حاضر بر روی 60</span><span dir="ltr"></span><span lang="AR-SA" style="font-family: "Times New Roman","serif" font-size: 9pt mso-bidi-font-family: "B Mitra" mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span> </span><span dir="rtl"></span><span lang="AR-SA" style="font-family: "B Mitra" font-size: 10pt mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 9.0pt"><span dir="rtl"></span><font size="2"><font face="B Mitra"><span style="mso-spacerun: yes"> </span>سر موش صحرایی نژاد ویستار نر بالغ که به طور تصادفی در 6 گروه شامل کنترل، دریافت کننده بوسولفان، پینئالکتومی، دریافت کننده بوسولفان و ملاتونین، دریافت کننده حلال بوسولفان </font></font></span><span dir="ltr"></span><span style="font-family: "Times New Roman","serif" font-size: 9pt mso-bidi-font-family: "B Mitra" mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span>(DMSO)</span><span dir="rtl"></span><span lang="AR-SA" style="font-family: "B Mitra" font-size: 10pt mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 9.0pt"><span dir="rtl"></span>، پینالکتومی، دریافت کننده بوسولفان و اتانول (حامل ملاتونین) و شم جراحی قرار گرفتند، انجام شده است. بوسولفان به صورت تک دوز (20 میلی گرم به ازای هر کیلوگرم) و ملاتونین به مدت 50 روز (5/0 میلی گرم به ازای هر کیلوگرم) به صورت داخل صفاقی (</span><span style="font-family: "Times New Roman","serif" font-size: 9pt mso-bidi-font-family: "B Mitra" mso-bidi-font-size: 10.0pt" dir="ltr">IP</span><span dir="rtl"></span><span lang="AR-SA" style="font-family: "B Mitra" font-size: 10pt mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 9.0pt"><span dir="rtl"></span>) تزریق گردید. پارامتر های اسپرمی شامل تعداد، مورفولوژی و تحرک اسپرم مورد بررسی قرار گرفته، از آن‌ها به روش </span><span style="font-family: "Times New Roman","serif" font-size: 9pt mso-bidi-font-family: "B Mitra" mso-bidi-font-size: 10.0pt" dir="ltr">IVF</span><span dir="rtl"></span><span lang="AR-SA" style="font-family: "B Mitra" font-size: 10pt mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 9.0pt"><span dir="rtl"></span> به منظور تعیین قدرت باروری استفاده شد. نتایج به دست آمده با استفاده نرم افزار </span><span style="font-family: "Times New Roman","serif" font-size: 9pt mso-bidi-font-family: "B Mitra" mso-bidi-font-size: 10.0pt" dir="ltr">SPSS</span><span dir="rtl"></span><span lang="AR-SA" style="font-family: "B Mitra" font-size: 10pt mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 9.0pt"><span dir="rtl"></span> نسخه 16 مورد آنالیز آماری قرار گرفتند. </span><span style="font-family: "B Mitra" font-size: 10pt mso-bidi-language: EN-US mso-ascii-font-family: "B Mitra" mso-hansi-font-family: "Times New Roman""><o:p /></span></p><p align="right"><font face="Times New Roman" size="3"> </font></p><p class="MsoNormal" style="margin: 0in 0in 0pt text-align: justify line-height: normal unicode-bidi: embed direction: rtl text-justify: kashida text-kashida: 0%" dir="rtl"><b><span lang="AR-SA" style="font-family: "B Mitra" font-size: 10pt mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 9.0pt">یافته‌ها: </span></b><span lang="AR-SA" style="font-family: "B Mitra" font-size: 10pt mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 9.0pt">بوسولفان</span><span dir="ltr"></span><span lang="AR-SA" style="font-family: "Times New Roman","serif" font-size: 9pt mso-bidi-font-family: "B Mitra" mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span> </span><span lang="AR-SA" style="font-family: "B Mitra" font-size: 10pt mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 9.0pt">باعث</span><span dir="ltr"></span><span lang="AR-SA" style="font-family: "Times New Roman","serif" font-size: 9pt mso-bidi-font-family: "B Mitra" mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span> </span><span lang="AR-SA" style="font-family: "B Mitra" font-size: 10pt mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 9.0pt">کاهش</span><span dir="ltr"></span><span lang="AR-SA" style="font-family: "Times New Roman","serif" font-size: 9pt mso-bidi-font-family: "B Mitra" mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span> </span><span lang="AR-SA" style="font-family: "B Mitra" font-size: 10pt mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 9.0pt">معنی دار</span><span dir="ltr"></span><span lang="AR-SA" style="font-family: "Times New Roman","serif" font-size: 9pt mso-bidi-font-family: "B Mitra" mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span> </span><span lang="AR-SA" style="font-family: "B Mitra" font-size: 10pt mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 9.0pt">در</span><span dir="ltr"></span><span lang="AR-SA" style="font-family: "Times New Roman","serif" font-size: 9pt mso-bidi-font-family: "B Mitra" mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span> </span><span lang="AR-SA" style="font-family: "B Mitra" font-size: 10pt mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 9.0pt">تعداد</span><span dir="ltr"></span><span lang="AR-SA" style="font-family: "Times New Roman","serif" font-size: 9pt mso-bidi-font-family: "B Mitra" mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span> </span><span lang="AR-SA" style="font-family: "B Mitra" font-size: 10pt mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 9.0pt">و</span><span dir="ltr"></span><span lang="AR-SA" style="font-family: "Times New Roman","serif" font-size: 9pt mso-bidi-font-family: "B Mitra" mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span> </span><span lang="AR-SA" style="font-family: "B Mitra" font-size: 10pt mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 9.0pt">تحرک</span><span dir="ltr"></span><span lang="AR-SA" style="font-family: "Times New Roman","serif" font-size: 9pt mso-bidi-font-family: "B Mitra" mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span> </span><span lang="AR-SA" style="font-family: "B Mitra" font-size: 10pt mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 9.0pt">و</span><span dir="ltr"></span><span lang="AR-SA" style="font-family: "Times New Roman","serif" font-size: 9pt mso-bidi-font-family: "B Mitra" mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span> </span><span lang="AR-SA" style="font-family: "B Mitra" font-size: 10pt mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 9.0pt">شکل</span><span dir="ltr"></span><span lang="AR-SA" style="font-family: "Times New Roman","serif" font-size: 9pt mso-bidi-font-family: "B Mitra" mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span> </span><span lang="AR-SA" style="font-family: "B Mitra" font-size: 10pt mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 9.0pt">طبیعی</span><span dir="ltr"></span><span lang="AR-SA" style="font-family: "Times New Roman","serif" font-size: 9pt mso-bidi-font-family: "B Mitra" mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span> </span><span lang="AR-SA" style="font-family: "B Mitra" font-size: 10pt mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 9.0pt">اسپرم</span><span dir="ltr"></span><span lang="AR-SA" style="font-family: "Times New Roman","serif" font-size: 9pt mso-bidi-font-family: "B Mitra" mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span> </span><span lang="AR-SA" style="font-family: "B Mitra" font-size: 10pt mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 9.0pt">در</span><span dir="ltr"></span><span lang="AR-SA" style="font-family: "Times New Roman","serif" font-size: 9pt mso-bidi-font-family: "B Mitra" mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span> </span><span lang="AR-SA" style="font-family: "B Mitra" font-size: 10pt mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 9.0pt">مقایسه</span><span dir="ltr"></span><span lang="AR-SA" style="font-family: "Times New Roman","serif" font-size: 9pt mso-bidi-font-family: "B Mitra" mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span> </span><span lang="AR-SA" style="font-family: "B Mitra" font-size: 10pt mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 9.0pt">با</span><span dir="ltr"></span><span lang="AR-SA" style="font-family: "Times New Roman","serif" font-size: 9pt mso-bidi-font-family: "B Mitra" mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span> </span><span lang="AR-SA" style="font-family: "B Mitra" font-size: 10pt mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 9.0pt">گروه کنترل شد (05/0</span><span style="font-family: "Times New Roman","serif" font-size: 9pt mso-bidi-font-family: "B Mitra" mso-bidi-font-size: 10.0pt" dir="ltr">p<</span><span dir="rtl"></span><span lang="AR-SA" style="font-family: "B Mitra" font-size: 10pt mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 9.0pt"><span dir="rtl"></span>). ملاتونین، باعث افزایش معنی‌دار در حفظ پارامترهای معنی دار در حفظ پارامترهای اسپرمی شد ولی بر بهبود قدرت باروری تاثیری نداشته است.</span><font size="3"><span lang="AR-SA" style="font-family: "B Mitra" mso-bidi-language: FA"> </span><span lang="FA" style="font-family: "B Mitra" mso-bidi-language: FA"><o:p /></span></font></p><p align="right"><font face="Times New Roman" size="3"> </font></p><p class="MsoNormal" style="margin: 0in 0in 0pt text-align: justify line-height: normal unicode-bidi: embed direction: rtl text-justify: kashida text-kashida: 0%" dir="rtl"><b><span lang="AR-SA" style="font-family: "B Mitra" font-size: 10pt mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 9.0pt">نتیجه</span></b><b><span style="font-family: "Times New Roman","serif" font-size: 9pt mso-bidi-font-size: 10.0pt" dir="ltr">‌</span></b><b><span lang="AR-SA" style="font-family: "B Mitra" font-size: 10pt mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 9.0pt">گیری</span></b><span lang="AR-SA" style="font-family: "B Mitra" font-size: 10pt mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 9.0pt">: بر اساس نتایج این تحقیق نتیجه گرفته می‌شود که ملاتونین درمانی می‌تواند در درمان برخی از نارسایی‌های تولید مثلی ناشی از شیمی درمانی در مذکر موثر باشد.<o:p /></span></p><p align="right"><font face="Times New Roman" size="3"> </font></p> <font face="Times New Roman" size="3"> </font><p class="MsoNormal" style="margin: 0in 0in 0pt text-align: justify line-height: normal text-justify: inter-ideograph tab-stops: center 265.9pt"><b><span style="font-family: "Times New Roman","serif" font-size: 12pt mso-bidi-language: FA">Background: </span></b><span style="font-family: "Times New Roman","serif" font-size: 12pt">Busulfan is drug of choice for treatment of certain cancers with well-known side effects. Infertility and testes tissue damage are known side effects. There is still no effective therapy to reduce these effects. Melatonin is a potent antioxidant with protective effects on certain tissues. The aim of this study was to evaluate the protective effects of exogenous melatonin on busulfan induced oigospermia and fertility of pinealectomized rat.</span><span style="mso-bidi-font-family: "B Mitra""><o:p /></span></p><font face="Times New Roman" size="3"> </font><p class="MsoNormal" style="margin: 0in 0in 0pt text-align: justify line-height: normal text-justify: inter-ideograph tab-stops: center 265.9pt"><b><span style="font-family: "Times New Roman","serif" font-size: 12pt">Methods</span></b><span style="font-family: "Times New Roman","serif" font-size: 12pt">: Sixty adult Wistar rats weighing 250-300 gr were used in this study. The animals were randomly divided into 6 groups: control, busulfan treatment, busulfan vehicle treatment, pinealectomized with busulfan and melatonin treatment, surgical sham, pinealectomized with busulfan and melatonin vehicle. Pinealectomy was done. Intraperitoneal injections of single dose of busulfan (20mg/kg) and melatonin (0.5mg/kg) for 50 days were administered.<font size="3"><font face="Times New Roman"><span style="mso-spacerun: yes"> </span>Animals were sacrificed and sperm parameters including sperm motility, count and morphology was evaluated. IVF technique was used to determine sperm fertility. The data were analyzed and presented in mean ± SD, Pv less than 0.05 was considered significant. <o:p /></font></font></span></p><font face="Times New Roman" size="3"> </font><p class="MsoNormal" style="margin: 0in 0in 0pt text-align: justify line-height: normal text-justify: inter-ideograph tab-stops: center 265.9pt"><b><span style="font-family: "Times New Roman","serif" font-size: 12pt">Results:</span></b><span style="font-family: "Times New Roman","serif" font-size: 12pt"> Busulfan significantly reduced sperm count, motility and fertility. It also caused sperm morphological changes (p<0<span dir="rtl"></span><span lang="AR-SA" dir="rtl"><span dir="rtl"></span><font face="Times New Roman" size="3">.</font></span><span dir="ltr"></span><span dir="ltr"></span>05). Melatonin treatment recovers the effects of busulfan on sperm parameters but not the fertility feature.</span><span style="mso-bidi-font-family: "B Mitra""><font size="3"><font face="Calibri"> <o:p /></font></font></span></p><font face="Times New Roman" size="3"> </font><p class="MsoNormal" style="margin: 0in 0in 0pt text-align: justify line-height: normal text-justify: inter-ideograph tab-stops: center 265.9pt"><b><span style="font-family: "Times New Roman","serif" font-size: 12pt">Conclusions: </span></b><span style="color: black font-family: "Times New Roman","serif" font-size: 12pt">Based on our results melatonin therapy might be useful to treat certain reproductive dysfunction in male followed by chemotherapy.<span lang="AR-SA" dir="rtl"><o:p /></span></span></p><font face="Times New Roman" size="3"> </font> غده پینئال، ملاتونین، الیگواسپرمی، بوسولفان. Pineal gland, Oligospermia, Busulfan, Melatonin. 77 86 http://rjms.iums.ac.ir/browse.php?a_code=A-10-1-1442&slc_lang=fa&sid=1 Ahade Ferdosi Khosroshahi احد فردوسی خسروشاهی 3900319475328460023778 3900319475328460023778 No Tabriz University of Medical Sciences دانشگاه علوم پزشکی تبریز Mehrdad Bakhtiari مهرداد بختیاری 3900319475328460023779 3900319475328460023779 No Iran University of Medical Sciences دانشگاه علوم پزشکی ایران Jafar Soleimani Rad جعفر سلیمانی راد 3900319475328460023780 3900319475328460023780 No Tabriz University of Medical Sciences دانشگاه علوم پزشکی تبریز Morteza Koroji مرتضی کروجی 3900319475328460023781 3900319475328460023781 No Iran University of Medical Sciences دانشگاه علوم پزشکی ایران Leila Roshangar لیلا روشنگر 3900319475328460023782 3900319475328460023782 No Tabriz University of Medical Sciences دانشگاه علوم پزشکی تبریز Atousa Janzadeh آتوسا جان زاده 3900319475328460023783 3900319475328460023783 No Iran University of Medical Sciences دانشگاه علوم پزشکی ایران Mahdieh Kerdari مهدیه کرداری 3900319475328460023784 3900319475328460023784 No Iran University of Medical Sciences دانشگاه علوم پزشکی ایران Behnamedin Jameie سید بهنام الدین جامعی 3900319475328460023785 3900319475328460023785 Yes Iran University of Medical Sciences دانشگاه علوم پزشکی ایران