fa بررسی اثر دیازینون بر آنزیم‌های آنتی‌اکسیدان و پراکسیداسیون لیپیدهای مغز موش صحرایی فیزیولوژی Physiology پژوهشي Research <h2 style="MARGIN: 0cm 0cm 0pt DIRECTION: rtl" dir="rtl"><p><span></span></p><p><span>    زمینه و هدف:</span><span dir="ltr"></span><span dir="ltr" lang="AR-SA"><span dir="ltr"></span><font face="Times New Roman">�</font></span><span style="FONT-FAMILY: " lang="AR-SA">ارگانوفسفره‌ها سبب افزایش تولید رادیکالهای آزاد و تخریب سیستم آنتی‌اکسیدانت بدن می‌گردند. دیازینون یکی از گسترده ترین ارگانوفسفره هایی است که امروزه در</span><span dir="ltr"></span><span dir="ltr" lang="AR-SA"><span dir="ltr"></span><font face="Times New Roman">�</font></span><span style="FONT-FAMILY: " lang="AR-SA">کشاورزی بکار می‌رود. هدف از این مطالعه ارزیابی اثر دیازینون روی سیستم اکسیدان- آنتی‌اکسیدان مغز موش صحرایی است.</span><h4 style="MARGIN: 0cm 0cm 0pt" dir="rtl"><span>روش کار: در</span><span dir="ltr"></span><span dir="ltr" lang="AR-SA"><span dir="ltr"></span><font face="Times New Roman">�</font></span><span style="FONT-FAMILY: " lang="AR-SA">مطالعه</span><span dir="ltr"></span><span dir="ltr" lang="AR-SA"><span dir="ltr"></span><font face="Times New Roman">�</font></span><span style="FONT-FAMILY: " lang="AR-SA">تجربی</span><span dir="ltr"></span><span dir="ltr" lang="AR-SA"><span dir="ltr"></span><font face="Times New Roman">�</font></span><span style="FONT-FAMILY: " lang="AR-SA">حاضر</span><span><span dir="ltr"></span><font face="Times New Roman">�</font></span><span style="FONT-FAMILY: " lang="AR-SA">موش</span><span style="FONT-FAMILY: " lang="AR-SA">‌های صحرایی</span><span dir="ltr"></span><span style="FONT-FAMILY: BMitra mso-bidi-font-family: 'B Yagut' mso-ascii-font-family: BMitra" dir="ltr" lang="AR-SA"><span dir="ltr"></span><font face="Times New Roman">�</font></span><span style="FONT-FAMILY: " lang="AR-SA">نر</span><span dir="ltr"></span><span style="FONT-FAMILY: BMitra mso-bidi-font-family: 'B Yagut' mso-ascii-font-family: BMitra" dir="ltr" lang="AR-SA"><span dir="ltr"></span><font face="Times New Roman">�</font></span><span style="FONT-FAMILY: " lang="AR-SA">نژاد</span><span dir="ltr"></span><span style="FONT-FAMILY: BMitra mso-bidi-font-family: 'B Yagut' mso-ascii-font-family: BMitra" dir="ltr" lang="AR-SA"><span dir="ltr"></span><font face="Times New Roman">�</font></span><span style="FONT-FAMILY: " lang="AR-SA">ویستار به</span><span style="FONT-FAMILY: " lang="AR-SA"> صورت تصادفی در 4</span><span dir="ltr"></span><span style="FONT-FAMILY: BMitra mso-bidi-font-family: 'B Yagut' mso-ascii-font-family: BMitra" dir="ltr" lang="AR-SA"><span dir="ltr"></span><font face="Times New Roman">�</font></span><span style="FONT-FAMILY: " lang="AR-SA">گروه</span><span dir="ltr"></span><span style="FONT-FAMILY: BMitra mso-bidi-font-family: 'B Yagut' mso-ascii-font-family: BMitra" dir="ltr" lang="AR-SA"><span dir="ltr"></span><font face="Times New Roman">�</font></span><span style="FONT-FAMILY: " lang="AR-SA">قرار گرفتند</span><span style="FONT-FAMILY: " lang="AR-SA">:</span><span dir="ltr"></span><span style="FONT-FAMILY: BMitra mso-bidi-font-family: 'B Yagut' mso-ascii-font-family: BMitra" dir="ltr" lang="AR-SA"><span dir="ltr"></span><font face="Times New Roman">�</font></span><span style="FONT-FAMILY: " lang="AR-SA">گروه کنترل (روغن ذرت بعنوان حلال دیازینون)</span><span dir="ltr"></span><span style="FONT-FAMILY: BMitra mso-bidi-font-family: 'B Yagut' mso-ascii-font-family: BMitra" dir="ltr" lang="AR-SA"><span dir="ltr"></span><font face="Times New Roman">�</font></span><span style="FONT-FAMILY: " lang="AR-SA">و</span><span dir="ltr"></span><span style="FONT-FAMILY: BMitra mso-bidi-font-family: 'B Yagut' mso-ascii-font-family: BMitra" dir="ltr" lang="AR-SA"><span dir="ltr"></span><font face="Times New Roman">�</font></span><span style="FONT-FAMILY: " lang="AR-SA">سه گروه</span><span dir="ltr"></span><span style="FONT-FAMILY: BMitra mso-bidi-font-family: 'B Yagut' mso-ascii-font-family: BMitra" dir="ltr" lang="AR-SA"><span dir="ltr"></span><font face="Times New Roman">�</font></span><span style="FONT-FAMILY: " lang="AR-SA">آزمایش که دیازینون را در دوزهای</span><font face="Times New Roman"><span dir="ltr">mg/kg </span><span dir="rtl"></span></font><span style="FONT-FAMILY: " lang="AR-SA"><span dir="rtl"></span><font face="B Yagut"><span style="mso-spacerun: yes"> </span>30، 50 و 100 </font></span><span style="FONT-FAMILY: " lang="AR-SA">بصورت </span><span style="FONT-FAMILY: " lang="AR-SA">داخل</span><span dir="ltr"></span><span style="FONT-FAMILY: BMitra mso-bidi-font-family: 'B Yagut' mso-ascii-font-family: BMitra" dir="ltr" lang="AR-SA"><span dir="ltr"></span><font face="Times New Roman">�</font></span><span style="FONT-FAMILY: " lang="AR-SA">صفاقی دریافت کردند. 24 ســاعت</span><span dir="ltr"></span><span style="FONT-FAMILY: BMitra mso-bidi-font-family: 'B Yagut' mso-ascii-font-family: BMitra" dir="ltr" lang="AR-SA"><span dir="ltr"></span><font face="Times New Roman">�</font></span><span style="FONT-FAMILY: " lang="AR-SA">پس</span><span dir="ltr"></span><span style="FONT-FAMILY: BMitra mso-bidi-font-family: 'B Yagut' mso-ascii-font-family: BMitra" dir="ltr" lang="AR-SA"><span dir="ltr"></span><font face="Times New Roman">�</font></span><span style="FONT-FAMILY: " lang="AR-SA">از</span><span dir="ltr"></span><span style="FONT-FAMILY: BMitra mso-bidi-font-family: 'B Yagut' mso-ascii-font-family: BMitra" dir="ltr" lang="AR-SA"><span dir="ltr"></span><font face="Times New Roman">�</font></span><span style="FONT-FAMILY: " lang="AR-SA">تزریق،</span><span dir="ltr"></span><span style="FONT-FAMILY: BMitra mso-bidi-font-family: 'B Yagut' mso-ascii-font-family: BMitra" dir="ltr" lang="AR-SA"><span dir="ltr"></span><font face="Times New Roman">�</font></span><span style="FONT-FAMILY: " lang="AR-SA">حیوانات</span><span dir="ltr"></span><span style="FONT-FAMILY: BMitra mso-bidi-font-family: 'B Yagut' mso-ascii-font-family: BMitra" dir="ltr" lang="AR-SA"><span dir="ltr"></span><font face="Times New Roman">�</font></span><span style="FONT-FAMILY: " lang="AR-SA">با</span><span dir="ltr"></span><span style="FONT-FAMILY: BMitra mso-bidi-font-family: 'B Yagut' mso-ascii-font-family: BMitra" dir="ltr" lang="AR-SA"><span dir="ltr"></span><font face="Times New Roman">�</font></span><span style="FONT-FAMILY: " lang="AR-SA">اتر بیهوش و</span><span dir="ltr"></span><span style="FONT-FAMILY: BMitra mso-bidi-font-family: 'B Yagut' mso-ascii-font-family: BMitra" dir="ltr" lang="AR-SA"><span dir="ltr"></span><font face="Times New Roman">�</font></span><span style="FONT-FAMILY: " lang="AR-SA">بافت</span><span dir="ltr"></span><span style="FONT-FAMILY: BMitra mso-bidi-font-family: 'B Yagut' mso-ascii-font-family: BMitra" dir="ltr" lang="AR-SA"><span dir="ltr"></span><font face="Times New Roman">�</font></span><span style="FONT-FAMILY: " lang="AR-SA">مغز</span><span dir="ltr"></span><span style="FONT-FAMILY: BMitra mso-bidi-font-family: 'B Yagut' mso-ascii-font-family: BMitra" dir="ltr" lang="AR-SA"><span dir="ltr"></span><font face="Times New Roman">�</font></span><span style="FONT-FAMILY: " lang="AR-SA">جدا</span><span dir="ltr"></span><span style="FONT-FAMILY: BMitra mso-bidi-font-family: 'B Yagut' mso-ascii-font-family: BMitra" dir="ltr" lang="AR-SA"><span dir="ltr"></span><font face="Times New Roman">�</font></span><span style="FONT-FAMILY: " lang="AR-SA">گردید. بعد از هموژنه کردن بافت مغز،</span><span dir="ltr"></span><span style="FONT-FAMILY: BMitra mso-bidi-font-family: 'B Yagut' mso-ascii-font-family: BMitra" dir="ltr" lang="AR-SA"><span dir="ltr"></span><font face="Times New Roman">�</font></span><span style="FONT-FAMILY: " lang="AR-SA">فعالیت</span><span dir="ltr"></span><span style="FONT-FAMILY: BMitra mso-bidi-font-family: 'B Yagut' mso-ascii-font-family: BMitra" dir="ltr" lang="AR-SA"><span dir="ltr"></span><font face="Times New Roman">�</font></span><span style="FONT-FAMILY: " lang="AR-SA">آنزیم‌های ســوپراکســیددیسموتاز (</span><font face="Times New Roman"><span style="FONT-FAMILY: TimesNewRomanPSMT" dir="ltr">SOD</span><span dir="rtl"></span></font><span style="FONT-FAMILY: " lang="AR-SA"><span dir="rtl"></span>)</span><span style="FONT-FAMILY: " lang="AR-SA">، کاتالاز (</span><font face="Times New Roman"><span style="FONT-FAMILY: TimesNewRomanPSMT" dir="ltr">CAT</span><span dir="rtl"></span></font><span style="FONT-FAMILY: " lang="AR-SA"><span dir="rtl"></span>)</span><span style="FONT-FAMILY: " lang="AR-SA">، </span><span style="FONT-FAMILY: " lang="AR-SA">گلوتاتیون </span><font face="Times New Roman"><span dir="ltr">S</span><span dir="rtl"></span></font><span style="FONT-FAMILY: " lang="AR-SA"><span dir="rtl"></span>- ترانسفراز </span><span style="FONT-FAMILY: " lang="AR-SA">(</span><font face="Times New Roman"><span dir="ltr">GST</span><span dir="rtl"></span></font><span style="FONT-FAMILY: " lang="AR-SA"><span dir="rtl"></span>) و لاکتات دهیدروژناز (</span><font face="Times New Roman"><span dir="ltr">LDH</span><span dir="rtl"></span></font><span style="FONT-FAMILY: " lang="AR-SA"><span dir="rtl"></span>)</span><span style="FONT-FAMILY: " lang="AR-SA"> و</span><span dir="ltr"></span><span style="FONT-FAMILY: BMitra mso-bidi-font-family: 'B Yagut' mso-ascii-font-family: BMitra" dir="ltr" lang="AR-SA"><span dir="ltr"></span><font face="Times New Roman">�</font></span><span style="FONT-FAMILY: " lang="AR-SA">میزان</span><span dir="ltr"></span><span style="FONT-FAMILY: BMitra mso-bidi-font-family: 'B Yagut' mso-ascii-font-family: BMitra" dir="ltr" lang="AR-SA"><span dir="ltr"></span><font face="Times New Roman">�</font></span><span style="FONT-FAMILY: " lang="AR-SA">گلوتاتیون (</span><font face="Times New Roman"><span style="FONT-FAMILY: TimesNewRomanPSMT" dir="ltr">GSH</span><span dir="rtl"></span></font><span style="FONT-FAMILY: " lang="AR-SA"><span dir="rtl"></span>) و</span><span dir="ltr"></span><span style="FONT-FAMILY: BMitra mso-bidi-font-family: 'B Yagut' mso-ascii-font-family: BMitra" dir="ltr" lang="AR-SA"><span dir="ltr"></span><font face="Times New Roman">�</font></span><span style="FONT-FAMILY: " lang="AR-SA">مالون</span><span dir="ltr"></span><span style="FONT-FAMILY: BMitra mso-bidi-font-family: 'B Yagut' mso-ascii-font-family: BMitra" dir="ltr" lang="AR-SA"><span dir="ltr"></span><font face="Times New Roman">�</font></span><span style="FONT-FAMILY: " lang="AR-SA">دی</span><span dir="ltr"></span><span style="FONT-FAMILY: BMitra mso-bidi-font-family: 'B Yagut' mso-ascii-font-family: BMitra" dir="ltr" lang="AR-SA"><span dir="ltr"></span><font face="Times New Roman">�</font></span><span style="FONT-FAMILY: " lang="AR-SA">آلدئید </span><span style="FONT-FAMILY: " lang="AR-SA">(</span><font face="Times New Roman"><span style="FONT-FAMILY: TimesNewRomanPSMT" dir="ltr">MDA</span><span dir="rtl"></span></font><span style="FONT-FAMILY: " lang="AR-SA"><span dir="rtl"></span>) توسط روش‌های بیوشیمیایی </span><span style="FONT-FAMILY: " lang="AR-SA">اندازه‌گیری شد.</span><span style="FONT-FAMILY: " lang="AR-SA"> معنی‌دار بودن نتایج توسط آنالیز واریانس (</span><font face="Times New Roman"><span dir="ltr">ANOVA</span><span dir="rtl"></span></font><span style="FONT-FAMILY: " lang="AR-SA"><span dir="rtl"></span>) به همراه تست توکی تعیین شد.<p></p></span></h4><h4 style="MARGIN: 0cm 0cm 0pt" dir="rtl"><span>یافته‌ها: به</span><span dir="ltr"></span><span dir="ltr" lang="AR-SA"><span dir="ltr"></span><font face="Times New Roman">�</font></span><span style="FONT-FAMILY: " lang="AR-SA">دنبال</span><span dir="ltr"></span><span dir="ltr" lang="AR-SA"><span dir="ltr"></span><font face="Times New Roman">�</font></span><span style="FONT-FAMILY: " lang="AR-SA">تجویز دیازینون، فعالیت آنزیم‌های </span><font face="Times New Roman"><span dir="ltr">SOD</span><span dir="rtl"></span></font><span style="FONT-FAMILY: " lang="AR-SA"><span dir="rtl"></span>،</span><span dir="ltr"></span><span dir="ltr"><span dir="ltr"></span><font face="Times New Roman"> GST </font></span><span style="FONT-FAMILY: " lang="AR-SA">و </span><font face="Times New Roman"><span dir="ltr">LDH</span><span dir="rtl"></span></font><span style="FONT-FAMILY: " lang="AR-SA"><span dir="rtl"></span> در دوزهای بیشتر از </span><font face="Times New Roman"><span dir="ltr">mg/kg</span><span dir="rtl"></span></font><span style="FONT-FAMILY: " lang="AR-SA"><span dir="rtl"></span> 50 در مقایسه با کنترل بطور معنی‌داری افزایش یافت، در حالیکه میزان</span><span dir="ltr"></span><span dir="ltr" lang="AR-SA"><span dir="ltr"></span><font face="Times New Roman">�</font></span><span style="FONT-FAMILY: " lang="AR-SA">فعالیت</span><span dir="ltr"></span><span dir="ltr" lang="AR-SA"><span dir="ltr"></span><font face="Times New Roman">�</font></span><span style="FONT-FAMILY: " lang="AR-SA">آنزیم</span><span dir="ltr"></span><span dir="ltr"><span dir="ltr"></span><font face="Times New Roman"><span style="mso-spacerun: yes">  </span>CAT </font></span><span style="FONT-FAMILY: " lang="AR-SA">تغییر معنی‌داری را نشان نداد. همچنین کاهش میزان گلوتاتیون مغز در دوزهای بیشتر از </span><font face="Times New Roman"><span dir="ltr">mg/kg</span><span dir="rtl"></span></font><span style="FONT-FAMILY: " lang="AR-SA"><span dir="rtl"></span> 50<font face="B Yagut"><span style="mso-spacerun: yes">  </span>دیازینون مشاهده شد. میزان </font></span><font face="Times New Roman"><span dir="ltr">MDA</span><span dir="rtl"></span></font><span style="FONT-FAMILY: " lang="AR-SA"><span dir="rtl"></span> فقط در دوز</span><span dir="ltr"></span><span dir="ltr"><span dir="ltr"></span><font face="Times New Roman"> mg/kg </font></span><span dir="rtl"></span><span style="FONT-FAMILY: " lang="AR-SA"><span dir="rtl"></span>100 در مقایسه با گروه کنترل افزایش معنی‌داری را نشان داد.<p></p></span></h4><strong><span dir="rtl" lang="AR-SA">نتیجه‌گیری:</span><span dir="ltr"></span></strong><span><span dir="ltr"></span><strong><font face="Times New Roman">�</font></strong>نتایج این مطالعه پیشنهاد می‌کند که دیازینون احتمالاً باعث تولید رادیکال‌های آزاد شده و افزایش فعالیت آنزیم‌های آنتی‌اکسیدانت ناشی از افزایش ظرفیت سم زدایی بافت مغز می‌باشد. کاهش </span><span>GSH</span><span dir="rtl"></span><span dir="rtl" lang="AR-SA"><span dir="rtl"></span> نشاندهنده نارسایی سیستم دفاعی آنتی‌اکسیدانت برای مقابله با رادیکال‌های آزاد است و افزایش </span><span>MDA</span><span dir="rtl"></span><span dir="rtl" lang="AR-SA"><span dir="rtl"></span> با پراکسیداسیون غشای مغز همراه است که در نهایت ممکن است منجر به آسیب اکسیداتیو بافت مغز گردد</span> </p></h2><p></p> <p style="MARGIN: 0cm 0cm 0pt" class="MatnLatin"><font face="Verdana"><span><strong></strong></span></font></p><p style="MARGIN: 0cm 0cm 0pt" class="MatnLatin"><font face="Verdana"><span><strong>    Background & Aim</strong>: Organophosphates (OPs) are capable to produce free radicals and induce disturbance in body antioxidant system. Diazinon is one of the most widely used OPs in agriculture. The aim of this study was to investigate the effects of diazinon on oxidant-antioxidant system in rat's brain tissue.</span><span style="FONT-FAMILY: Arial mso-ascii-font-family: Verdana mso-hansi-font-family: Verdana" dir="rtl" lang="AR-SA"></span></font><p></p><p style="MARGIN: 0cm 0cm 0pt" class="MatnLatin"><font face="Verdana"><b><span lang="EN">Material and Method: </span></b><span lang="EN">In this experimental study, male Wistar rats were randomly divided into four groups as follows: control group(corn oil as diazinon solvent) and three case groups<span style="mso-spacerun: yes">  </span>receiving diazinon at different doses (30, 50 and 100 mg/kg) via intraperitoneal injection. 24 hours after injection, the <span style="mso-spacerun: yes"> </span>animals were anesthetized and their brain tissues were removed. After brain tissue hemogenation, superoxide dismutase (SOD) , catalase (CAT), lactate dehydrogenase (LDH) and glutathione S-transferase (GST) activities as well as glutathione (GSH) and malondialdehyde (MDA) levels were determined by biochemical methods</span><span style="FONT-SIZE: 11pt" lang="EN">.</span><span lang="EN">�</span><span style="mso-bidi-font-family: Times-Roman" lang="EN">The data were statistically analyzed using analysis of variance (ANOVA) followed by post hoc analysis using Tukey test.</span></font></p><p style="MARGIN: 0cm 0cm 0pt" class="MatnLatin"><font face="Verdana"><b><span lang="EN">Results:</span></b><span lang="EN"> Diazinon increased SOD, LDH and GST activities at doses of 50 and 100 mg/kg<span style="mso-spacerun: yes">  </span>compared with the control group but decreased GSH level. There were no significant changes observed in brain CAT activity. Also, MDA concentration was significantly increased at 100 mg/kg dose in comparison with the control group.</span></font></p><p style="MARGIN: 0cm 0cm 0pt" class="MatnLatin"><font face="Verdana"><b><span lang="EN">Conclusion:</span></b><span lang="EN"> The results suggest that diazinon probably causes free radical production. Enhanced activity of antioxidant enzymes and depleted GSH content are indicative of oxidative tissue injury and the increased MDA level is indicative of the damage that occurs in the membranes of brain tissues as a result of free radical generation.</span></font></p><p style="MARGIN: 0cm 0cm 0pt" class="MatnLatin"><span lang="EN"></span><p></p></p><p><font face="Verdana"> </font></p></p><p></p> کلیدواژه‌ها: 1- دیازینون 2- سیستم آنتی‌اکسیدانت 3- موش صحرایی 4- بافت مغز Key Words: 1) Diazinon 2) Antioxidant System 3) Rat 4) Brain Tissue 15 23 http://rjms.iums.ac.ir/browse.php?a_code=A-10-1-949&slc_lang=fa&sid=1 M Salehi مریم صالحی 3900319475328460016531 3900319475328460016531 No M Jafari مهوش جعفری 3900319475328460016532 3900319475328460016532 Yes A Asgari علیرضا عسگری 3900319475328460016533 3900319475328460016533 No M Saleh Moghaddam مسعود صالح مقدم 3900319475328460016534 3900319475328460016534 No Department of Biochemistry. Payame Noor University واحد علوم پایه، دانشگاه پیام نور M Salimian محمد سلیمیان 3900319475328460016535 3900319475328460016535 No M Abbasnejad مریم عباس نژاد 3900319475328460016536 3900319475328460016536 No Department of Biochemistry. Payame Noor University واحد علوم پایه، دانشگاه پیام نور M Haj Gholamali منصوره حاج غلامعلی 3900319475328460016537 3900319475328460016537 No