TY - JOUR JF - RJMS JO - RJMS VL - 27 IS - 5 PY - 2020 Y1 - 2020/7/01 TI - The effect of high intensity interval training on serum monocyte chemotactic protein and intercellular adhesion molecule after resveratrol supplementation in rat TT - تاثیرهشت هفته تمرین اینتروال شدید بر پروتئین جاذب شیمیایی مونوسیت (MCP1) و مولکول چسبان سلولی (ICAM1 ) پس از مکمل سازی با رزوراترول در رت N2 - Background: High-intensity interval training can improve aerobic power especially if they have active recovery periods .So that the athlete will be able to perform endurance activities at a higher speed. Immune, oxidative and inflammatory markers are factors that may appear with exercise and if there is no proper recovery period, they will have negative effects. One of these factors is Monocyte chemotactic protein-1, also known as factor 1, a chemical adsorbent for monocytes, and is produced by monocytes, endothelial cells, fibroblasts, and T cells. This factor increases the migration of monocytes from the bloodstream to the tissue and converts them into macrophages, and by inducing phagocytosis, it eliminates the infection from the body. Cell adhesion molecule is another factor. These molecules are glycoprotein receptors that are present on the outer surfaces of the cell membrane and can pass through the membrane and enter the cytoplasm. Adhesive molecules are important in directing the movement of white blood cells as well as their exit from the bloodstream to the lymphatic tissues, etc., especially areas of infection and inflammation. Some of these molecules are soluble in plasma and their presence indicates the degree of vascular endothelial dysfunction. Methods: The research method was experimental with an applied purpose. The study population was male Sprague-Dawley rats. Among them, sixty rats were selected for the study and randomly divided into four groups (interval training, interval training + supplement, resveratrol supplement and control). The training program was performed for eight weeks and five sessions per week. Resveratrol supplementation was done daily in the supplement and supplement + exercise groups. Resveratrol supplementation was given orally with a gavage syringe once a day for eight weeks in the supplement and supplement + exercise groups. In exercise and resveratrol groups, physiological serum was used to equalize gavage stress. The supplement and control groups had no exercise. Blood samples were taken 48 hours after the last training session and 10-12 hours of fasting in different stages and with similar conditions. Rats were profoundly anesthetized by a skilled veterinarian in accordance with ethical principles by intra peritoneal injection of a combination of ketamine and xylazine. At the end of the period, 5 ml of blood were taken from rats to measure the biochemical factors of Intercellular Adhesion Molecule-1 index. Blood samples were taken directly from the left ventricle of their hearts. The serum of the samples were then separated and kept at -70 ° C until the measurement of serum samples. Serum samples were separated by centrifugation for 15 minutes at 30 ° C and transferred to the laboratory to evaluate and evaluate changes. Visceral adipose tissue was also sampled to measure Monocyte chemotactic protein-1 protein concentration. An incision was made in the abdomen of the rats and the white visceral fat tissue were removed. White subcutaneous fat tissue were obtained from the groin just above the thigh. The resulting fat samples were immediately frozen in nitrogen and stored in the refrigerator at -80 ° C for subsequent measurements. At the end of the study period, the rats were facilitated. Blood samples were then transferred to a reference laboratory for analyzes. First the normality of the distribution of the main variables was checked using Shapiro-Wilk test and the homogeneity of variances was checked by Leven test. Mean, standard deviation statistical tests and one-way analysis of variance were used to determine changes. Then, Bonferroni post hoc test was used to analyze the data. All results were analyzed using SPSS and excel statistical software. Results: The highest mean for Monocyte chemotactic protein-1 and Intercellular Adhesion Molecule-1belonged to the interval intensity training group. The results showed that interval training had the highest increase on Monocyte chemotactic protein-1 and Intercellular Adhesion Molecule-1 and the largest decrease is in the resveratrol group. Changes Monocyte chemotactic protein-1 and Intercellular Adhesion Molecule-1 are significantly. There was a statistically significant difference between resveratrol supplementation, resveratrol supplementation and resveratrol supplementation + intense interval training groups with the control group. There was a statistically significant difference in the groups of interval intensity training, resveratrol supplementation and resveratrol supplementation + interval intensity training with the control group. Monocyte chemotactic protein-1 and Intercellular Adhesion Molecule-1 between the control group and the interval intensity training group with resveratrol supplementation were confirmed by Bonferroni test post hock (p≤0.05). Conclusion: Monocyte chemotactic protein-1 of changes, showed that resveratrol + intensity interval training had a significant effect and reduced monocyte chemotactic protein-1. It is possible that the reduction in fat volume due to the training protocol was effective in reducing Monocyte chemotactic protein-1, and the anaerobic exercises designed in the protocol cause the production of free radicals and increased oxidative stress. Oxidative damage plays an important role in the production of inflammatory factors and mediators and strengthens the immune system and exercise training induced indicators inflammations including Monocyte chemotactic protein-1. Resveratrol supplementation with antioxidant properties has been able to enhances a wide range of biological functions focused on regulating oxidation and inflammation, and reduce pro-inflammatory cytokine levels and inflammatory responses. Resveratrol probably protects cells through its high antioxidant properties. The balance of oxidants and antioxidants is an important indicator of immune system function, and any disturbance in this balance causes oxidative stress. Therefore, the concentration of food antioxidants in immune cells is higher than other cells, and a lack of antioxidants disrupts the immune response. The protocol exercise considered in the study may have caused infection and pathogenic and harmful factors in the research samples. Which is activated and strengthened by the effect of resveratrol on the immune system and the intrinsic part of the acquisition, followed by a decrease in Monocyte chemotactic protein-1. The role of monocytes in the release of proteases from lysosomes and the destruction of oxygen and nitrous oxide free radicals is also debatable. Because it eliminates infectious factors and pathogens, it produces IL-8 and Monocyte chemotactic protein-1 cytokines. They activate lymphocytes and stimulate the inflammatory process to improve .The results showed that interval training with resveratrol decreased Intercellular Adhesion Molecule-1 and less adhesive molecules than the training group from endothelial secretion and adequate safety is provided. It is possible that the selected exercise was also involved in the study to reduce the weight and fatty tissue of the samples and helped to reduce Intercellular Adhesion Molecule-1. Exercise reduces inflammation and adhesion of leukocytes and platelets to the endothelium. Another effect of the training protocol could be the reduction of cell adhesion molecule synthesis agonists in adipose tissue. The role of resveratrol as a compound that improves and strengthens the immune system in the production of cytokines and anti-inflammatory agents and strengthens the production of immune cells is also significant. Improving the antioxidant status and increasing the oxidative capacity and counteracting free radicals has led to the development of the immune system and a decrease in the Intercellular Adhesion Molecule-1 index in the endothelium. It is recommended to use less intensity interval training and reduce the negative effects by using resveratrol if you use this type of training. Protocol exercise and resveratrol can reduce or prevent inflammation and strengthen the immune system. The natural and non-synthetic resveratrol is another factor that will develop the use of this compound among patients and athletes. Resveratrol supplementation improves the performance of beginner and professional athletes as well as cardiovascular, diabetic and morbidly obese patients on various parts of the body with metabolic effects, protection of the immune system and counteracting the effects of exercise and the side effects of vascular disease and obesity can work well. It is also recommended to use fruits and vegetables containing resveratrol such as grapes, chard and raspberries. The use of resveratrol supplementation and its effect on immune factors such as CRP, interleukins and HSPs may also be useful for future research. SP - 36 EP - 48 AU - Alidusti, Javad AU - Nameni, Farah AD - Department,Varamin Pishva Branch, Islamic Azad University, Varamin, Iran KW - Interval training KW - Resveratrol KW - MCP.1 KW - ICAM.1 UR - http://rjms.iums.ac.ir/article-1-5768-en.html ER -