RT - Journal Article T1 - Effect of Aqueous Extract of the Aerial Part of the Ruta Graveolens on the Spermatogenesis of Immature Balb/C Mice JF - RJMS YR - 2007 JO - RJMS VO - 14 IS - 56 UR - http://rjms.iums.ac.ir/article-1-768-en.html SP - 13 EP - 20 K1 - 1) Aqueous Extract 2) Ruta Graveolens 3) Spermatogenesis 4) Immature Balb/C Mice AB -     Background & Aim: Ruta graveolens(R.G) has been used for sexual impotence in men and abortion in women. In this study the effect of aqueous extract of this plant has been investigated on testis tissue and spermatogenesis. Material and Methods: For this reason as an experimental research, animals were allocated to three groups as control which did not receive anything, vehicle which received only normal saline, and experimental which received ruta extract. Lethal dose 50(LD 50) of extract was considered as 560mg/kg. Based on this, sub LD 50 dose of aqeous extract i.e 280mg/kg was injected intraperitoneally once every other day for one week. A month after the last injection the animals were deeply anesthetized and dissected. Blood was collected intracardically for hormonal assay. The testis were extruded, weighed and then fixed for histological studies. For statistical analysis ANOVA and Tukey as a post hoc test were used. Results: Intraperitoneal injection of 280mg/kg R.G showed nonsignificant changes in body weight and testicular weight and induced a significant decrease in the number of type A spermatogonia(P LA eng UL http://rjms.iums.ac.ir/article-1-768-en.html M3 ER -