RT - Journal Article T1 - ADENYLYL CYCLASE ASSAY TO MEASURE MACROPHAGE CHEMOTACTIC PROTEIN-1 RECEPTOR FUNCTION JF - RJMS YR - 2001 JO - RJMS VO - 8 IS - 25 UR - http://rjms.iums.ac.ir/article-1-348-en.html SP - 283 EP - 287 K1 - 1) Adenylyl cyclase 2) Macrophage chemotactic protein-1 AB -  Adenylyl cyclase is a membrane-bound enzyme that catalyzes the conversion of ATP to cAMP. The inhibition of adenylyl cyclase was carried out by measuring the ability of the macrophage chemotactic protein-1 to inhibit the forskolin-induced enzyme activity. Measurement of adenylyl cyclase activity was performed according to the procedure described by Wiegn.  Adenylyl cyclase activity in the present macrophage chemotactic protein-1 was decreased compared to that in controls [2.11+/- 0.15(mean +/-SD.)vs. 6.83+/-0.45, activity ( µ mol cAMP/mg protein/min)].  Macrophage chemotactic protein significant reduced adenylyl cyclase activity by 79.9%. LA eng UL http://rjms.iums.ac.ir/article-1-348-en.html M3 ER -