RT - Journal Article T1 - Study of glycoconjugates terminal sugars during gonadogenesis in rat by lectin histochemical method JF - RJMS YR - 2011 JO - RJMS VO - 18 IS - 82 UR - http://rjms.iums.ac.ir/article-1-1632-en.html SP - 44 EP - 53 K1 - Gonadogenesis K1 - Development K1 - Glycoconjugates K1 - Lectin K1 - L-Fucos K1 - N-Accetyle Galactose. AB -   Introduction : Introduction: Gonadogenesis is a complex developmental process which is regulated by molecular interactions such as cell surface and extracellular matrix (ECM) Glycoconjugates terminal sugars. The aim of this study was to determine Glycoconjugates terminal sugars expressions and changes during Gonadogenesis in Rat using Lectin histochemical technique.   Methods : To determine of glycoconjugate terminal sugers by Lectin histochemistry method, thirty Wistar Rat embryos at different stages from days 12 to 16 of gestation were fixed in formalin, embedded in paraffin and cut in to 5µm thickness sections serially. The sections were incubated with different HRP- lectins from Lotus tetragonolobus (LTA), Maclura pomifera(MPA) and Arachis hypogaea or Peanut (PNA), that are specific for terminal sugars L-Fuc (α2-4)GlcNAc, Gal(β1-3)GalNAc and D-Gal(β1-3)DGalNAc respectively. On the basis of colorimetery data that was determined by blind’s method, sections were graded and SPSS statistic soft ware and kruscal Wallis tests were used for comparing different embryonic stages.   Results : Our finding showed that LTA did not react with developing gonads at gestational day 12(E12). LTA reactivity was found in extracellular matrix (ECM) of developing gonads from E14 through E16 (P LA eng UL http://rjms.iums.ac.ir/article-1-1632-en.html M3 ER -