Volume 31, Issue 1 (3-2024)
RJMS 2024, 31(1): 0-0 |
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Research code: 27481
Ethics code: IR.SBMU.PHARMACY.REC.1400.032
Clinical trials code: 0
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Barabadi H, Vahidi H, Ameri Shah Reza M, Soltani M, Jounaki K, Sadeghian-Abadi S et al . Biosynthesis of silver and selenium nanoparticles using Lentinus edodes mushroom supernatant and structural characterization and evaluation of their antioxidant activity. RJMS 2024; 31 (1)
URL: http://rjms.iums.ac.ir/article-1-8398-en.html
URL: http://rjms.iums.ac.ir/article-1-8398-en.html
Hamed Barabadi 
, Hossein Vahidi , Mahdieh Ameri Shah Reza , Maha Soltani , Kamyar Jounaki , Salar Sadeghian-Abadi , Maryam Babaei Sabet
, Hossein Vahidi , Mahdieh Ameri Shah Reza , Maha Soltani , Kamyar Jounaki , Salar Sadeghian-Abadi , Maryam Babaei Sabet
Shahid Beheshti University of Medical Sciences , barabadi.87@gmail.com
Abstract: (322 Views)
Background & Aims: Nanobiotechnology implicates the integration between biotechnology and nanotechnology. Biosynthesis of metal nanoparticles in the range of 1 to 100 nm is one of the topics of interest for researchers in nanotechnology due to the lack of environmental pollution and cost-effectiveness. Among different approaches for the biogenic fabrication of metal-based nanoparticles, the fungus-mediated fabrication of nanoparticles is of high interest. Some of the fungi are toxic and not safe for humans. Hence, exploring the new safe fungi that can fabricate metal nanoparticles with specific size distribution and morphology is a significant and promising aspect of the biosynthesis of nanoparticles. Lentinus edodes is a popular and medicinal edible mushroom in the global market with wide therapeutic properties. This study aimed to investigate the ability of extracellular synthesis of silver and selenium nanoparticles using the supernatant of Lentinus edodes. In the next step, the antioxidant properties of these mycosynthesized nanoparticles were compared.
Method: Initially, Lentinus edodes was cultured in Sabouraud Dextrose Broth (SDB) medium and the grown fungi were isolated from the culture medium. Then, 100 mL of 1 mM metal salt solution (silver and selenium) was added to 100 mL of supernatant and incubated at 28 °C for 48 hours. The fabrication of silver and selenium nanoparticles in the supernatant was investigated. In the next step, the biosynthesis of nanoparticles was investigated through macroscopic observations by the color change of the reaction system and the Tyndall effect. Then, the characteristics of biosynthesized nanoparticles were evaluated by employing Field emission scanning electron microscopy (FE-SEM), Dynamic light scattering (DLS), and Fourier-transform infrared spectroscopy (FT-IR). Finally, the antioxidant effect of the produced nanoparticles was evaluated by DPPH free radical scavenging assay.
Results: Initially, the color change to brown and orange confirmed the fabrication of silver and selenium nanoparticles, respectively. Then, the Tyndall effect confirmed the formation of a colloidal system. In the next step, the mycofabrication of nanoparticles was confirmed by employing DLS, FE-SEM, and FT-IR. Lentinus edodes was able to biosynthesize the silver and selenium nanoparticles with an average hydrodynamic diameter of 91.79 and 85.75 nm and a Polydispersity index (PdI) of 0.56 and 0.434 at pH value of 10, respectively. Based on FE-SEM images, nanoparticles were synthesized with a spherical morphology. The FT-IR analysis showed the presence of different functional groups on the surface of the synthesized nanoparticles. These functional groups are attributed to the conjugated biomolecules that surround the nanoparticles. These fungal biomolecules not only stabilize the colloidal system of nanoparticles, but also influence the biological performance of nanoparticles. Furthermore, silver and selenium nanoparticles at the concentration of 0.5 mM inhibited 49.04±1.5 and 49.81±1.79% of DPPH free radicals, respectively.
Conclusion: This study showed that Lentinus edodes was able to synthesize silver and selenium nanoparticles extracellularly as a reducing agent by reducing silver and selenium ions. The mycosynthesized nanoparticles also showed significant antioxidant activity. Several parameters such as particle size, morphology, surface chemistry, and capping agents influence the biological properties of nanosized particles. Although these nanoparticles exhibited significant in vitro antioxidant performance, further in vivo studies are required to demonstrate the antioxidant properties of these nanoparticles. Moreover, future animal-based investigations should be conducted to determine the pharmacokinetics and pharmacodynamics of these nanoparticles.
Method: Initially, Lentinus edodes was cultured in Sabouraud Dextrose Broth (SDB) medium and the grown fungi were isolated from the culture medium. Then, 100 mL of 1 mM metal salt solution (silver and selenium) was added to 100 mL of supernatant and incubated at 28 °C for 48 hours. The fabrication of silver and selenium nanoparticles in the supernatant was investigated. In the next step, the biosynthesis of nanoparticles was investigated through macroscopic observations by the color change of the reaction system and the Tyndall effect. Then, the characteristics of biosynthesized nanoparticles were evaluated by employing Field emission scanning electron microscopy (FE-SEM), Dynamic light scattering (DLS), and Fourier-transform infrared spectroscopy (FT-IR). Finally, the antioxidant effect of the produced nanoparticles was evaluated by DPPH free radical scavenging assay.
Results: Initially, the color change to brown and orange confirmed the fabrication of silver and selenium nanoparticles, respectively. Then, the Tyndall effect confirmed the formation of a colloidal system. In the next step, the mycofabrication of nanoparticles was confirmed by employing DLS, FE-SEM, and FT-IR. Lentinus edodes was able to biosynthesize the silver and selenium nanoparticles with an average hydrodynamic diameter of 91.79 and 85.75 nm and a Polydispersity index (PdI) of 0.56 and 0.434 at pH value of 10, respectively. Based on FE-SEM images, nanoparticles were synthesized with a spherical morphology. The FT-IR analysis showed the presence of different functional groups on the surface of the synthesized nanoparticles. These functional groups are attributed to the conjugated biomolecules that surround the nanoparticles. These fungal biomolecules not only stabilize the colloidal system of nanoparticles, but also influence the biological performance of nanoparticles. Furthermore, silver and selenium nanoparticles at the concentration of 0.5 mM inhibited 49.04±1.5 and 49.81±1.79% of DPPH free radicals, respectively.
Conclusion: This study showed that Lentinus edodes was able to synthesize silver and selenium nanoparticles extracellularly as a reducing agent by reducing silver and selenium ions. The mycosynthesized nanoparticles also showed significant antioxidant activity. Several parameters such as particle size, morphology, surface chemistry, and capping agents influence the biological properties of nanosized particles. Although these nanoparticles exhibited significant in vitro antioxidant performance, further in vivo studies are required to demonstrate the antioxidant properties of these nanoparticles. Moreover, future animal-based investigations should be conducted to determine the pharmacokinetics and pharmacodynamics of these nanoparticles.
Keywords: Biological synthesis, Selenium nanoparticles, Silver nanoparticles, Lentinus edodes, Antioxidant effect
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