Research code: 175
Ethics code: IR.ARUMS.REC.1400.175
Clinical trials code: پژوهش بروی نمونه حیوانی بوده است
University of Mohaghegh Ardabili , a.pourrahim@uma.ac.ir
Abstract: (157 Views)
- Background
Diabetes is the most common metabolic disease (1). High blood pressure is also one of the complications of dangerous diseases. High blood pressure is associated with increased risk of high cholesterol, insulin resistance, prevalence of type 2 diabetes and chronic kidney disease (4). Kidneys have a very strong regulatory effect on blood pressure through controlling and regulating the balance of body fluids. Renin-angiotensin system, aldosterone as an endocrine system with kidney-derived renin regulates the production of angiotensin II (5). Renin by itself does not really affect resting blood pressure. Instead, it converts the inactive form of angiotensin into angiotensin I (6). Angiotensin I is able to change blood pressure to some extent, but most of the angiotensin I is converted to angiotensin II, which is a much stronger hormone that causes large changes in blood pressure on the adrenals. Aldosterone is the most important mineralocorticoid in the body, which is secreted from the cortical part of the adrenal gland and is important in controlling blood pressure (5). Therefore, one of the goals of drug and non-drug treatments (exercise) is to reduce blood pressure (10). Exercise is one of the factors affecting the components of metabolic syndrome, especially the homeostasis of sugar and fat (11). Exercise also lowers blood pressure in people with mild to moderate hypertension. Exercise and physical activities not only improve health, but can prevent many diseases, including blood pressure. Therefore, despite the contradictory results in previous studies and the lack of sufficient studies regarding the effects of exercise training against induced blood pressure in diabetic individuals, the purpose of this study is to investigate the effect of combined exercise on the serum levels of renin, angiotensin and aldosterone in diabetic Wistar rats with It is streptozocin.
- Methods
At the beginning of the research, the animals were 6 weeks old with an average weight of 250 ± 5 grams. The rats were kept in groups of 10 under temperature conditions (22±2°C), 12-hour light-dark period. From the number of 30 male Wistar rats, 20 male Wistar rats after induction of diabetes by injecting 55 mg/kg streptozocin dissolved in citrate buffer (0.05 M with pH=4.5) (20) randomly in two Diabetic training group (n=10) and diabetic control group (n=10) and 10 healthy rats were included in the healthy control group (n=10). To ensure that the rats become diabetic, their blood sugar was measured 72 hours after the injection with the help of a glucometer (Active model, Accu-Chek company, made in Germany) and a blood sample taken from the tail vein of the rats. and blood sugar more than 250 mg/dL was considered as an indicator of type 2 diabetes (21). To implement the training program, two weeks after becoming diabetic, the training group performed the training protocol with an intensity of 70-85 maximum oxygen consumption for eight weeks (5 days a week and every day for 40 minutes at a speed of 10 meters per minute on the treadmill and climbed the ladder 15 times with a weight equal to 3% of body weight to the tail of the rodent). To perform endurance-strength training, first, the training started at a speed of 15 m/min and with an intensity of 40% of VO2max for 15 minutes on a treadmill with a 0-degree incline in the first session. At the end of the third week, the speed of the treadmill was fixed at 17 m/min and the duration of the exercise was increased to 30 minutes and 40 minutes in the second and third weeks, respectively. The speed and duration of running in the fourth week until the end of the eighth week were fixed at 20 meters per minute and 40 minutes respectively, and with zero-degree night until the end of the training sessions. The speed at which the blood lactate level reaches above 6 ml/liter was considered as the maximum speed. This test was done twice at the beginning and at the end of the experiment at the same time of the day (21). Resistance training, after endurance training, was done for 8 weeks, for 5 days a week in the form of climbing and climbing stairs, to In the first, second, and third weeks, the rats climbed 8, 10, and 12 times in each session with weights equal to zero percent of the rats' body weight, which were attached to the animal's tail. From the fourth week. Until the end of the eighth week, the rats climbed 15 times a week by carrying weights equal to 3% of the total body weight of the subjects (22). In addition, at the beginning of each session, the rats had 5 minutes of warm-up exercise (with an intensity of 10 meters per minute). and at the end, they worked for 5 minutes to cool down (intensity 10 meters per minute and gradually reducing the intensity to the lowest value) (25). Also, the healthy control group who did not participate in any activity program and did not become diabetic. Creating the same conditions as other training groups, 5 days a week for 10 to 15 minutes in each session, they were placed on the motionless treadmill to adapt to the environment.
- Result
The results of the one-way Anova test showed that at the end of eight weeks, between body weight (P=0.043), BMI (P=0.043), renin (P=0.0001), angiotensin (P=0.0001) and aldosterone (P=0.0001) there is a significant difference between exercise-diabetic, diabetic control and healthy control groups (Table 2). The serum level of renin in the diabetic exercise group had a significant decrease of 36.59% compared to the diabetic control group (P=0.0001) and a significant increase of 47.58% in the diabetic control group compared to the healthy control group (P=0.0001). /0=P). The serum level of angiotensin decreased by 20.27% in the diabetic exercise group compared to the diabetic control group, which was not significant (P=0.150). On the other hand, there was a significant increase of 174.01% in the diabetic control group compared to the healthy control group (P=0.0001). The serum level of aldosterone in the diabetic training group decreased by 9.46% compared to the diabetic control group, which was not significant (P=0.531). On the other hand, there was a significant increase of 175.88% in the diabetic control group compared to the healthy control group (P=0.0001) (Table 3).
- Conclusion
The findings of the present research showed that eight weeks of combined endurance-strength training caused a significant decrease in serum renin levels. While, there was no significant change in the serum levels of angiotensin, aldosterone, weight, height and BMI in the diabetic exercise group compared to the diabetic control group. The results of the current research and the studies conducted in this field indicate that exercise is effective in preventing high blood pressure caused by diabetes. Therefore, more research should be done to investigate the effect of exercise on the indicators of blood pressure and prevention of high blood pressure and diabetes, using human and animal subjects and in both males and females and in a longer period of time.
Type of Study:
Research |
Subject:
Exercise Physiology
, Ameneh PourRahimGhouroghchi 