Volume 32, Issue 1 (3-2025)
RJMS 2025, 32(1): 1-11 |
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Ghazanfari Hashemi M. Analysis of Single Nucleotide Changes in People with Polycystic Ovary Syndrome by Tetra ARMS-PCR Method. RJMS 2025; 32 (1) :1-11
URL: http://rjms.iums.ac.ir/article-1-8176-en.html
URL: http://rjms.iums.ac.ir/article-1-8176-en.html
Department of Radiology, Cancer Institute, Tehran University of Medical Sciences, Tehran, Iran, Department of Radiology, Cancer Institute, Tehran University of Medical Sciences, Tehran, Iran , Mohamadghhashemi@gmail.com
Abstract: (71 Views)
Abstract
Background and Aim: Polycystic ovary syndrome (PCOS) is a disorder that affects the sex hormones of women of reproductive age. As the name of the syndrome suggests, when ovulation does not occur, the ovaries are involved, thereby disrupting the normal hormone cycle and causing the uterine wall to be exposed to a constant level of estrogen, thus, the endometrial layer becomes too thick and causes bleeding. It becomes abnormal in women. These changes may lead to endometrial cancer or precancerous changes. Also, not ovulating regularly can cause infertility. It should be noted that women with PCOS are more commonly involved in metabolic syndromes such as abdominal obesity, high cholesterol, high blood pressure, and insulin resistance, or diabetes, the presence of any of these symptoms also increases the risk of heart disease. Importantly, obesity is common in women with PCOS. The causes of this syndrome in women can be genetic or environmental. Among the genetic factors studied, several gene polymorphisms including CYP2 or INS3 genes can be mentioned. Nowadays, the discussion of biomarkers and micro RNAs is also discussed and studies have been done in this field.
Methods: Investigating the expression of miRNA-155, miRNA-383, and miRNA-9 biomarkers by qRT-PCR method and analysis of single nucleotide changes rs1894116, rs2272046, and rs2252673 in Yap1, HMGA2, and INSR genes in people with polycystic ovary syndrome (PCOS) Tetra ARMS-PCR method has been performed.
Results: The total number of people studied in this study was 200 people, with an average age of 29-42 years and a range of 18-46 years. A case-control study was conducted in which 100 healthy women with an average age of 49-31 and 100 women with polycystic ovary syndrome with an average age of 21-29 were randomly selected. The comparison of the average age of the two groups showed that the difference There is a statistically significant difference between the average age of these two groups (p<0.001).
Conclusion: The research conducted on nucleotides dirs2272046 and rs2252673 in Yap1, HMGA2, and INSR genes of these genotypes are related to PCOS, in addition, the expression of selected micro RNAs miRNA-9, miRNA-383, and miRNA-155 show that all three of these micro RNAs are significantly increased in PCOS and they can be used as biomarkers for diagnosis.
Background and Aim: Polycystic ovary syndrome (PCOS) is a disorder that affects the sex hormones of women of reproductive age. As the name of the syndrome suggests, when ovulation does not occur, the ovaries are involved, thereby disrupting the normal hormone cycle and causing the uterine wall to be exposed to a constant level of estrogen, thus, the endometrial layer becomes too thick and causes bleeding. It becomes abnormal in women. These changes may lead to endometrial cancer or precancerous changes. Also, not ovulating regularly can cause infertility. It should be noted that women with PCOS are more commonly involved in metabolic syndromes such as abdominal obesity, high cholesterol, high blood pressure, and insulin resistance, or diabetes, the presence of any of these symptoms also increases the risk of heart disease. Importantly, obesity is common in women with PCOS. The causes of this syndrome in women can be genetic or environmental. Among the genetic factors studied, several gene polymorphisms including CYP2 or INS3 genes can be mentioned. Nowadays, the discussion of biomarkers and micro RNAs is also discussed and studies have been done in this field.
Methods: Investigating the expression of miRNA-155, miRNA-383, and miRNA-9 biomarkers by qRT-PCR method and analysis of single nucleotide changes rs1894116, rs2272046, and rs2252673 in Yap1, HMGA2, and INSR genes in people with polycystic ovary syndrome (PCOS) Tetra ARMS-PCR method has been performed.
Results: The total number of people studied in this study was 200 people, with an average age of 29-42 years and a range of 18-46 years. A case-control study was conducted in which 100 healthy women with an average age of 49-31 and 100 women with polycystic ovary syndrome with an average age of 21-29 were randomly selected. The comparison of the average age of the two groups showed that the difference There is a statistically significant difference between the average age of these two groups (p<0.001).
Conclusion: The research conducted on nucleotides dirs2272046 and rs2252673 in Yap1, HMGA2, and INSR genes of these genotypes are related to PCOS, in addition, the expression of selected micro RNAs miRNA-9, miRNA-383, and miRNA-155 show that all three of these micro RNAs are significantly increased in PCOS and they can be used as biomarkers for diagnosis.
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