Volume 24, Issue 164 (2-2018)
RJMS 2018, 24(164): 85-92 |
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Boroujerd Branch, Islamic Azad University, Boroujerd, Iran , r.shirvani1368@gmail.com
Abstract: (3432 Views)
Background: Enterococci are part of normal flora of the human gastrointestinal tract, which have a high potential for acquiring antibiotic resistance genes. The widespread use of antibiotics in the agricultural and food industriel leads to release of resistance genes in pathogens and normal flora bacteria, transfer to human and also the cause of the disease, which is a serious menace to human health. So, the purpose of this study was evaluation prevalence of bla CTX-M gene in gentamicin resistant Enterococcus faecalis isolated from consumption red meats.
Methods: In this cross-sectional study 190 Enterococcus isolates were collected from consumption red meats Boroujerd Slaughterhouse. 90 E.faecalis strains were identified by routine microbiological tests. The antibiotic susceptibility testing on isolates by disk diffusion method according to the CLSI was performed. Finally, to assess the frequency of bla CTX-M gene in gentamicin resistant Enterococcus faecalis isolated from consumption red meats were performed by using PCR and specific primer.
Results: In this study, the highest antibiotic resistance was observed against erythromycin and penicillin (94.44% and 56.66% respectively), strains had a high sensitivity to nitrofurantoin, meropenem and ciprofloxacin.Out of 90 samples 9(10%) were resistant to gentamicin, the gene was not found in any of the 9 strains.
Conclusion: Given that the genes producing Betalactamase enzymes are easily transferred via plasmids, lack of tracking the mentioned genes among the studied bacteria suggests, this gene is not transferred along with genes inducing resistance to gentamicin. Thus significant relationship between these gene and investigated antibiotic was not observed
Methods: In this cross-sectional study 190 Enterococcus isolates were collected from consumption red meats Boroujerd Slaughterhouse. 90 E.faecalis strains were identified by routine microbiological tests. The antibiotic susceptibility testing on isolates by disk diffusion method according to the CLSI was performed. Finally, to assess the frequency of bla CTX-M gene in gentamicin resistant Enterococcus faecalis isolated from consumption red meats were performed by using PCR and specific primer.
Results: In this study, the highest antibiotic resistance was observed against erythromycin and penicillin (94.44% and 56.66% respectively), strains had a high sensitivity to nitrofurantoin, meropenem and ciprofloxacin.Out of 90 samples 9(10%) were resistant to gentamicin, the gene was not found in any of the 9 strains.
Conclusion: Given that the genes producing Betalactamase enzymes are easily transferred via plasmids, lack of tracking the mentioned genes among the studied bacteria suggests, this gene is not transferred along with genes inducing resistance to gentamicin. Thus significant relationship between these gene and investigated antibiotic was not observed
Type of Study: Research |
Subject:
Microbiology
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