Volume 21, Issue 129 (3-2015)                   RJMS 2015, 21(129): 1-9 | Back to browse issues page

XML Persian Abstract Print

Zanjan University of Medical Sciences, Zanjan, Iran
Abstract:   (6700 Views)

Background: More than 90% of women use drugs during pregnancy. The aim of this study was to investigate oral morphine-induced histopathological changes in Wistar rat fetuses’ liver.


Methods: Animals were divided to 1 control and 3 drug level induced groups. 0.1, 0.2 and 0.3 mg/ml of morphine sulphate (99.98%) in drinking water (25 ml) were administered orally to pregnant rats from day 8 to 20. On gestation day 21, fetuses were removed surgically and after removing liver of fetuses, samples of tissue stained by hematoxylin and eosin (H&E) method and studied under light microscope. The data evaluated by SPSS software, ANOVA and analysis varieties.


Results: In the experimental groups with dose 0.1, 0.2 and 0.3 mg/ml of morphine, liver sections appeared with vacuolated hepatocytes, dilated sinusoids, and increased number of kupffer cells, lymphocytes and neutrophils. The results in experimental groups with doses 0.2 and 0.3mg/ml of morphine were significant comparing with control group (p≤ 0.001).

Conclusion: The results indicate that the lowest dose of morphine (0.1mg/ml) induced very low toxicity in fetuses but the middle dose (0.2mg/ml) and the high dose of morphine (0.3mg/ml) induced adverse reactions. It is concluded that oral morphine sulphate induces significant histopathological changes in the liver tissue, and the severity of these changes increases with time, period and dosage
Full-Text [PDF 417 kb]   (3409 Downloads)    
Type of Study: Research | Subject: Anatomy

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.