ABSTRACT Nucleolar organizer regions(NORs) are loops of DNA included ribosomal genes that ribosomal RNA translated of it, which synthesis proteins. NORs are in short arms of acrocentric chromosomes and associated with argyrophilic proteins, which are seen as black dots, termed “AgNOR”, by using colloidal silver (Ag) staining method. AgNOR count in nucleous have direct relation with cellular proliferative activity. The significance of this study is to evaluate the potential value of AgNOR in differentiating benign from malignant lesions, particullary, on the slide prepared from small biopsy, Frozen section, FNA and touch imprint, that are difficult to definite diagnosis by routin H&E staining. In this study, AgNOR stained on 90 section of paraffin embeded blocks included, 45 benign lesions and 45 malignant lesions. The average number of NORs in benign lesion and malignant tumor were 4.13 and 9.82 respectively. By using t-test and one way ANOVA-test, significant statistical difference is between benign and malignant lesions, so AgNOR staining is a simple, rapid and cheap method in difinite diagnosis of benign from malignant breast lesions.

Keywords: 1) Nucleolar organizer regions(NOR) 2) AgNOR Staining Method 3) Benign lesion of breast 4) Malignant lesion of breast

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