Infection may be caused by transfer of bacteria from the hands of the surgical team during operative procedures. Therefore carefull surgical scrubbing is performed to reduce the number of bacteria on the skin. The duration of scrubbing and the antiseptic solution are two variables in this purpose. One hundered sample were removed from the index pulp of Right hand of the surgeons. At the time of zero, 2, 4, 6 and eight minutes after hand scrubbing with betadyn and brush. After incubation of the samples, culture results were reported. This study showed that 2 minutes scrubbing the hand by povidine iodine %7.5, would probably be enough to reduce the number of the bacteria of the skin.

Keywords: Key Words: 1) Hand scrubbing 2) Antiseptic solution 3) Skin culture

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