39 2228-7043 Iran University of Medical Sciences 3893 Cardio Muscular Disease Response of interleukin 6, necrosis factor alpha and cortisol to intensive exercise in athlete’s women 1 7 2015 22 133 1 7 01 09 2015 01 09 2015 Background: Studies show that changes in inflammatory cytokines associated with inflammatory, hormonal changes in women has been less studied, especially in female athletes. So aim of this research is the investigation of Response of interleukin 6, necrosis factor alpha and cortisol to intensive exercise in athlete’s women. Methods: Among the volunteers, 15 subjects allowed to participate in the study and then participated in Alstad exercise test (gradient: 15%, Speed: 8 mph, time: 16 minutes). Blood sample were taken in three stages: pre exercise test, immediately and 1 hour after (recovery) of exercise test. Results: cortisol, IL-6 and TNF-α levels were significantly increased at all stages exercise training (At all stages: p=0.001). CRP levels did not significantly change immediately after exercise, but significantly decreased 1 hour after exercise (p=0.04). Conclusion: intensive exercise increases inflammatory cytokines and cortisol levels in athlete’s women. So it seems that during the intense exercise athletes female are confronted with injuries of inflammatory markers (cytokines and inflammatory hormones).
3894 Pathology Evaluating the expression of putative stem cell markers ALDH1 and CD133 in melanoma cell lines A375 and D10 1 7 2015 22 133 8 15 01 09 2015 01 09 2015 Background: The cancer stem cells (CSCs) are involved in invasion and metastasis of melanoma, the most lethal and aggressive form of skin cancer. In the present study, the expression of putative stem cell markers CD133 and ALDH1 were evaluated in melanoma cell lines and then the cells sorted based on the expression of these markers. Methods: In the present study expression of CD133 and ALDH1 was evaluated in A375 and D10 cell lines using flow cytometry. Then, selected cell line was sorted up on the expression of selected stem cell marker into positive and negative populations. Results: Our results indicated that ALDH1 marker expressed in 55.71 ± 7.88% and 49.88 ± 6.43% in A375 and D10 cell lines, respectively, while the CD133 only expressed in D10 cells in 26.76 ± 2.83%. Cell sorting was performed according to ALDH1 expression in both cell lines and CD133 expression only in D10 cells. The purity of isolated cells was high according to CD133 expression (90% <), whereas the purity of ALDH1+ and ALDH1- populations was very low. Conclusion: Findings of this study showed that cell sorting based on stem cell marker CD133 was suitable in D10 cells, therefore this marker could be a reliable marker for isolating of CSCs in this cell line.   3895 Endocrinology & Metabolism Effects of Caffeine, L-Carnitine and Their Combination on 400m Freestyle Performance in Adolescent Female Swimmers 1 7 2015 22 133 16 26 01 09 2015 01 09 2015 Background: The study aim was to examine the effect of caffeine, L-carnitine, and their combination on lactate blood level, and exercise time during a 400m freestyle swimming trial. Methods: In a double-blind randomly designed study, 16 Karaj female freestyle swimmers aged 13.87±1.14 yr, BMI 21.69±4.34 kg/m2, height 160.87±58.75cm, weight 56.31±1.31kg were recruited. The trial activities included completing maximal 400m freestyle. The activity was performed half an hour after taking caffeine (3 mg/kg body weight) or L-carnitine (1.5g), caffeine + L-carnitine (3 mg/kg + 1.5 g), or placebo on 4 similar separate occasions. Blood lactate was measured 1 and 10 min after exercise. Results: Repeated measures ANOVA indicated that no significant difference was observed for performance in  all groups (p≥0.05), whereas significant differences were observed in lactate accumulation in 1 min after trial (p<0.05). Conclusion: These findings showed that consuming caffeine, L-carnitine and their combination has no meaningful effect on performance. However, it seems that taking caffeine alone or with L-carnitine result would result in more lactate accumulation in 1 min after exercise.   3896 Orthopedic Surgery Single injection of platelet-rich plasma in knee osteoarthritis: a pilot study 1 7 2015 22 133 27 34 01 09 2015 01 09 2015 Background: Platelet Rich Plasma (PRP) contains Growth Factors (GFs) and antiinflammatory agents with possibility of application in regeneration of damaged cartilage in the treatment of osteoarthritis (OA). In the most trials, three injections of PRP have been done but in this research we assessed the efficacy and safety of single injection of PRP in the management of OA. Methods: In this single-center, uncontrolled, prospective preliminary study, 10 patients with knee OA who met the study criteria received single PRP injections. Outcome measures included the Visual Analog Scale (VAS), Knee Injury, Osteoarthritis Outcome (KOOS) questionnaire and 20 meters walk test at pre injection visit, 3 week, 2 and 6 month follow up visits. Results: There were no adverse events. The study demonstrated significant improvements in VAS, 20 meters walk test and some KOOS scores, including pain relief and better function in daily living (ADL) but some subscale such as symptoms relief and sport activities and quality of life did not significantly improved. Conclusion: The study showed positive results of single PRP injection and safety profile. Therefore, it could potentially be used to larger, blinded, and randomized clinical trial to determine if PRP is more effective than other treatments of knee OA or not. 3897 Pulmonary Disease Relation of serum Vitamin D level and pulmonary punction in COPD patients 1 7 2015 22 133 35 40 01 09 2015 01 09 2015 Background: The aim of this study was to determine the mean of the serum level of vitamin D in chronic obstructive pulmonary disease (COPD) patients referred to Rasoul-E-Akram Hospital, Tehran, Iran in 2010-11. Methods: In this sectional study 56 patients with COPD were recruited. The COPD condition was confirmed in these patients following spirometry. Serum vitamin D level of all patients was measured and categorized according to the 5 stages of 0,1,2,3, and 4 suggested by Global Initiative on Obstructive Lung Disease (GOLD). The data were analyzed using SPSS v. 18. Results: The mean age of patients was 68.1 ± 10.3. About%76 of patients were men. The study indicated that%85.1 of the patients were suffering from vitamin D deficiency with the level less than 30 mg /dl. Also, it was indicated that there is a direct and linear relation between serum vitamin D level and forced expiratory volume in one second (FEV1) volume(p= 0.03), (B= 0.4). Conclusion: The study demonstrated that vitamin D deficiency in COPD patients is highly prevalent. Thus, diagnostic examinations of vitamin D deficiency and on time treatment can prevent from many related problems in these patients. 3898 Infectious Disease Lived experience of Iranian women with HIV/AIDS and acknowledgment of their social rights: a phenomenological hermeneutical study 1 7 2015 22 133 41 50 01 09 2015 01 09 2015 Background: In Iran, there has been a considerable increase in the number of women infected with the human immunodeficiency virus (HIV). Within a socio-political context, social rights inequality of HIV positivity is still a prevailing concern among women. Little is known about the understanding of how Iranian women living with HIV, live their social rights experience. The aim of this study was to examine lived experience of these women against their social rights. Methods: In this qualitative study, a phenomenological–hermeneutic framework was adopted. We conducted narrative interviews with sixteen women. A purposeful sample of Iranian women was chosen. Results: Five themes were identified to understand the experience of social rights in women: 1) A state of profound suffering 2) lack support 3) Insecurity in a social-economic life 4) Any respecting and 5) Exerting oneself to keeping. For these women, social rights by their HIV status meant: Living the ambivalence of a process of equality/inequality. Conclusion: Health professionals have to broaden their role and work on individual, interpersonal and inter-organizational levels. Mobilization of actors from different sectors would facilitate the implementation of pertinent and opportune interventions. 3899 Hematology & oncology The effect of estrogen on p53 protein in T47D breast cancer cell line 1 7 2015 22 133 51 58 01 09 2015 01 09 2015 Background: Breast cancer is one of the most common cancers in women. Nearly 30% of breast cancers are hormone-dependent, and these hormones comprising estrogens influence progression of breast cancers. It is now widely recognized that p53 may be the most frequently mutated protein in breast cancer. High levels of p53 protein are a common feature of many human malignant cancers. Given that, T47D cell line is estrogen and progesterone receptor positive and p53 protein is one of the most important tumor suppressor genes. This article examines the effect of estrogen on p53 protein in T47D. Methods: The human breast cancer T47D cell line were cultured in 25cm2 flasks in DMEM medium supplemented with fetal bovine serum (FBS) and treated with different concentrations (3, 6, and 9 nmol) of estrogen. The levels of proteins were measured by western blot method. Gene tool software and One Way ANOVA were used for statistical analysis. Results: Comparison of p53 levels in T47D cell line showed that cells that were exposed to 3, 6, and9 nmol of estrogen treatment had higher concentration of p53 than control (p<0.001). Conclusion: The results showed that estrogen can strongly increase p53 protein concentration in T47D cell line. Therefore, it seems that estrogen can cause protein over expression and accumulation in T47D cell line.   3900 Pediatric Neurology Electroencephalography in children with simple, complex, and recurrent febrile seizures 1 7 2015 22 133 59 63 01 09 2015 01 09 2015 Background: Febrile seizures (FS) occur in 5% of children with 6 to 60 months ages. Many investigations are being performed, one of them is EEG. This study was done to determine EEG in recurrent simple and complex febrile seizures. Methods: In this descriptive, cross sectional study, we evaluated 86 patients with recurrent and complex febrile seizure admitted to neurology ward in Ali-Asghar training children hospital between 2008 and 2012. Data were recorded on age, gender, type of seizure (focal, generalized), and family history of febrile seizure, epilepsy, and EEG results. Results: Eighty six patients had EEG. Mean (SD) age was 24.27±15.28 months. Forty three (50%) cases were 6-18 month of age. Fifty three (62%) cases were male. Thirty three (38.5%) patients had recurrent simple FS and 53 (61.5%) had complex FS. Twenty nine (37.7%) cases had family history of FS and 13(15%) cases had family history of epilepsy. Thirty six percent of cases had abnormal EEGs. Abnormal finding included slow waves (14%), sharp waves (16%) and spike waves (6%). There was significant relation between abnormal EEGs and age (p=0.04), though no meaningful relation with gender, type of seizures and family history of FS or epilepsy. Conclusion: Seizures during a febrile illness are common in children under 5 years of age. Due to changes in EEG in children with simple, complex, and recurrent FS, performing EEG in these cases is recommended. 3901 Microbiology Detection of Oseltamivir Resistant Influenza A/H3N2 viruses by Real-time RT-PCR 1 7 2015 22 133 64 69 01 09 2015 01 09 2015 Background: Currently, with increasing risk of influenza A virus epidemics, a lot of studies have been performed. Oseltamivir or Tamiflu (the neuraminidase (NA) inhibitor) is one of the effective drugs for preventing and treatment of these viruses. The H274Y mutation is from the most important drug resistant factors in influenza A viruses. The aim of this study was detection of Oseltamivir resistant influenza A/H3N2 viruses by 274 position inspection using Real-time RT-PCR. Methods: Initially, specific primers and probs for detection of sensitive and resistant A/H3N2 viruses were designed. The Real-time RT-PCR assay was performed to detect mutation in 274 position of NA gene. Results: Of 50 A/H3N2 specimens, all were negative for H274Y mutation and no resistant viruses were selected. Conclusion: Quick and accurate recognition of drug resistant mutants is necessary for effective treatment strategies. Real-time RT-PCR assay is a rapid operational test which could be performed in the laboratories for detection of influenza viruses resistant to NA inhibitor.   3902 Exercise Physiology The study of immunoglobulin A, G and cortisol serum response in two consecutive soccer match and vitamin C supplements 1 7 2015 22 133 70 79 01 09 2015 01 09 2015 Background: Regarding importance of anti-oxidative nutrition in recovery period especially in relation with strengthening the immune system, the aim of this study was to investigate the effect two consecutive soccer matches with vitamin C supplementation during recovery on serum immunoglobulin and cortisol in collegiate male footballers. Methods: A sampling of 36 soccer footballers from football leagues first division of Iranian universities were selected and randomly divided in to three groups. supplementation group (SG) to perform two consecutive football games and receive one-liter beverage containing 500 mg vitamin C, placebo group (PG) also performed two consecutive football games and received  the same amount of aspartame-containing beverage, while the control group (CG) did not receive any intervention. Every football match consists of two 45- minute halves with 15 minute rest time in between and was held the interval of less than 48 hours of the next contest. Supplements were consumed in period 1 hour after the second games. Blood samples were collected before and one hour after second games for measurement measuring of the relevant indicators items. The paired and independent t-test was used to assess significant within and between groups to analyze the results. Results: As can be seen in the table, the changes within the groups of IgA and cortisol (p=0.023) is significant (p=0.04) in the placebo group. Conclusion: Overall, it seems appears that vitamin C supplementation in the form of effervescent tablets in during recovery  from two consecutive soccer games can moderated  change  in serum immunoglobulin A, G  and cortisol levels in college football players   and  may reduce the incidence of URTI in these players. 3903 Biology The effects of barley grain (Hordeum Vulgare L.) during pregnancy on development and heart histopathological changes of embryos of diabetic rats 1 7 2015 22 133 80 88 01 09 2015 01 09 2015 Background: Diabetes mellitus in pregnancy can cause heart histological changes. This study was done to evaluate the effect of barly grain (Hordeum Vulgar L.) consumption during pregnancy in diabetic rats on heart histological altrations of offsprings. Methods: In this experimental study ,60 adult female albino rats, randomly allocated into four groups including: healthy group with regular meals consumption as control, healthy which consumed barley( 10 grams per each rat per daily), diabetic with regular meals consumption and diabetic group which consumed barley( 10 grams per each rat per daily). diabetes was induced by intraperitoneal injection of 45 mg/kg/bw of streptozotocin. After confirmation of pregnancy by observing the vaginal plug on 21th days, the dams were anesthetized and embryos were removed. Crown rump length and weight of embryos wre recorded.after heart tissue processing, sections with 5 micrometer thickness were stained with H;E method. Results: Cardiomyocytes  from  change  was  seen  as  hypertrophy,  interstitial  plates  and  Purkinje  fibers reduction,  inflammation  and  congestion  of  vessels were observed in embryos of diabeticts group. These tissues alterations significantly reduced in the embryos of diabetic  group  which  consumed  barley.  The crown rump length of embryos significantly reduced in diabetic groupin comparision with controls. There was no many differences in crown rump length of embryos between diabetic consumed barley and diabetic group. The weight of embryos was non-significantly more in diabetic groups than controls. The weight of embryos reducednon-significantly in diabetic plus barley consumptionin comparision with controls.  Conclusion: The consumption of barley is beneficial in reducing heart histological alterations in embryos of diabetic rats.