39 2228-7043 Iran University of Medical Sciences 2742 Pathology Assessment of relationship between , Body Mass Index (BMI) and serum levels of Prostate Specific Antigen (PSA) in 40-79 year old men referred to Rasoul akram hospital Zare-Mirzaie Ali b Ahmadi Seyedeh jahan c Shayanfar Nasrin d Behrang Kazeminegad Behrang e b Iran University of Medical Sciences c Iran University of Medical Sciences d Iran University of Medical Sciences e Shahid Beheshti University of Medical Sciences 1 4 2015 22 130 1 7 21 10 2013 14 07 2014   Abstract Background: The level of Prostate-Specific Antigen (PSA) in identifying patients at high risk for prostate cancer has always been a controversial issue. This study was designed in order to assess the relationship between PSA levels with age and Body Mass Index (BMI) in symptom free men. Methods: In this cross sectional study, demographic data and PSA levels in 180 men referred to Rasoul Akram Hospital with analysis, data description, chi-square and correlation tests with SPSS software were used. Results: The mean age of men was 60.7±9 years and the mean of BMI was 26.1±3.5 kg/m2. This study showed inverse correlation between PSA and BMI which was very low and insignificant (p=0.141). But the correlation between PSA and age were significant and positive. Conclusion: This study showed there is no correlation between PSA and BMI.  However studies which have been conducted in symptomatic patients have shown a correlation between PSA and BMI. This may result from PSA level above 10 and leading to the ambiguity that it is perhaps due to high levels of PSA.
3718 Immunology Effects of Echinacea pupurea on the immunity system: from promise to fact Abtahi Froushani Seyyed Meysam f Gheibi Shahsanam g Esmaeili Gouvarchin Ghaleh Hadi h Mansori Motlagh Bahman i f Urmia University, Urmia, Iran. meysamabtahi@hotmail.com g Urmia University of Medical Sciences, Urmia, Iran. h Urmia University of Medical Sciences, Urmia, Iran. i Urmia University of Medical Sciences, Urmia, Iran. 1 4 2015 22 130 8 15 30 05 2015 30 05 2015 Background: Echinacea purpurea has long been used in folk medicine to prevent and treat a wide range of diseases like common cold, simple cough, pulmonary infections, dermatologic disorder and even chronic diseases due to immunodeficiency. This study was conducted to check the immunomodulatory properties of hydroalcoholic extract of Echinacea purpurea in NMRI-mice challenged with Sheep Red Blood Cells (SRBCs). Methods: The study population included 14 NMRI-male mice that were randomly grouped in two equal groups and immunized with SRBC. Hydroalcoholic extract of Echinacea pupurea were administered to the treatment group mice per os in daily doses of 0.025 mg from the beginning of the study and continued for 2 weeks. Specific humoral and cellular immunity, susceptibility of macrophages respiratory burst and proliferation of immune cells were measured. The Mann-Whitney test was used in order to compare using SPSS 19 software. Results: The findings indicated a significant increase (205.267±10.933) in the level of anti-SRBC antibody and simultaneously a significant decrease (15.288±4.202) in the level of cellular immunity in treatment group compared to control group. Lymphocyte proliferation index in splenocytes was significantly increased (1.821±227) in treatment group. However, the level of respiratory burst intensity (0.702±0.020) and nitric oxide production (50.253±2.032) in phagocytic population of splenocytes dramatically decreased in treatment groups compared to control mice. Conclusion: Since, the respiratory burst and nitric oxide production of phagocytic cells decreased, the possible immunostimolatory effectiveness of Echinacea pupurea is doubtful. However, this data suggest that the hydroalcoholic extract of Echinacea pupurea may be used as a natural source for purposes of modulating the immune system. 2325 Orthopedic Surgery Result of first metatarsal proximal osteotomy and Mc bride procedure in the treatment of Hallux valgus defomity yegane ali j mottaghi arash k tabe-arjmand kamal l shahhoseini gholamreza m Farahani Hoseine n j Department of Orthopaedics, Hazrate-rasol Hospital, Tehran University of Medical Sciences, Tehran, Iran. k Department of Orthopaedics, Besat Hospital, Hamedan University of Medical Sciences, Hamedan, Iran. l Department of Orthopaedics, Hazrate-rasol Hospital, Tehran University of Medical Sciences, Tehran, Iran. m Department of Orthopaedics, Hazrate-rasol Hospital, Tehran University of Medical Sciences, Tehran, Iran. n Department of Orthopaedics, Hazrate-rasol Hospital, Tehran University of Medical Sciences, Tehran, Iran. 1 4 2015 22 130 16 20 17 12 2012 02 08 2014 Background: Hallux valgus deformity is a common and debilitating disease. Proximal osteotomy of first metatarsal and Mc Bride b:union:ectomy procedure is a common procedure for this deformity.the goal of this study is to evaluate the result of this operation in Rasoul-e-akram hospital in Tehran. Methods: 50 cases of hallux valgus deformity   in 48 patients over a 5 years period who have been treated with this method were evaluated in Rasoul-e-akram hospital in Tehran. In this study mean of pre and post hallux valgus angle (HVA), intermetatarsal angle (IMA), and, pain were compared with each and also patient satisfaction and complications were evaluated Results: The mean angles correction for hallux valgus (HVA), intermetatarsal (IMA) were 27.9 and 6.5 degrees respectively. Patient satisfaction was good in 28 patients and there was meaningfull correlation between angle correction and Patient satisfaction (p<0.05).mean age of patiens was 41. In 38 (79%) patients post-operative pain decreased and in 10 (20%) patients arch of motion decreased. In 4 patients (8%) subtle mal:union: were noted.there were no cases of metatarsal head osteonecrosis, non:union:, hallux varus deformity, and infection. Conclusion: Proximal first metatarsal osteotomy and Mc Bride b:union:ectomy procedure is a reasonable procedure in Patints with moderate to severe hallux valgus deformity considering its low complication rate and good result in this study. Residual Pain and first MTP joint decreased arch of motion are the two main side effects. 3146 Biostatistics Survival analysis of Colorectal Cancer patients and its Prognostic Factors using Cox Regression ROUDBARI MASOUD o ABBASI ASL MOHSEN p BARFEI FARZANE GOHARI MAHMOOD REZA KHODABAKHSHI REZA o IRAN UNIVERSITY OF MEDICAL SCIENCES p IRAN UNIVERSITY OF MEDICAL SCIENCES IRAN UNIVERSITY OF MEDICAL SCIENCES IRAN UNIVERSITY OF MEDICAL SCIENCES FAYYAZBAKHSH HOSPITAL 1 4 2015 22 130 21 28 23 06 2014 02 08 2014 Background: Colorectal cancer is the third current cancer in the world and the forth cause of death in cancers. Certain factors such as environmental, genetic and life style are related with this cancer. The objective of this study is to find the survival of Iranian patients with colorectal cancer and also to find its prognostic factors. Methods: In this survival study, the data was collected from colorectal cancer patients who had referred to Fayyazbakhsh Hospital in Tehran from 2007 to 2009, using their hospital records. The final situation of the patients was recorded in 2012. For survival analysis of the patients, Kaplan-Meier method was used. The prognostic factors were calculated using Cox Regression. Results: The median of survival for the patients was (38.8±0.35) month. The one and 5 years survival rates were 79.2% and 39.6%, respectively. The effect of metastasis in prognostic time, age, having surgery and grading were significant using multivariate analysis. The high age, having surgery and the increase of virulent degree were the causes of patients’ survival reduction using Cox regression. Conclusion: The five year survival rate is less than western countries. Since there are many young colorectal cancer patients in the country, it is possible to increase their life using screening tests and early diagnostic programs. 2918 Exercise Physiology effect of High intensity interval training on hormonal factor affected on bone metabolism ghardashi afousi alireza khashayar patrishiya gaeini abbasali choobineh siroce fallahi aliasghar javidi mohsen Faculty of Physical Education and Sport Sciences, Tehran University Osteoporosis Research Center, Tehran University of Medical Sciences Faculty of Physical Education and Sport Sciences, Tehran University Faculty of Physical Education and Sport Sciences, Tehran University university of shiraz Faculty of Physical Education and Sport Sciences, University of shahid Beheshti 1 4 2015 22 130 29 37 17 02 2014 02 08 2014 Background: High interval training changes the bone metabolism. The present study was aimed at investigating the effect of High Intensity Interval Training (HIIT) on biochemical factor of adult male Wistar rat. Methods: In this semi-experimentral studu  sixteen male Wistar rats aged 6 weeks old with  weight of 250±20 g were divided into control (n=8) and experimental groups (n=8). After a week of orientation training protocol, eight weeks of incremental HIIT training program was followed. 24 hours after the last training session, anesthesia and sacrificed blood samples were taken directly from the animal's heart. Serum PTH and ALP were determined with RAI (Imonotopic CA kit), kinetic (Pars azmon kit), Ca and P were determined with Arsenazo, Phosphomolydate and independent t-test was used for statistical analysis. Results: The result indicated that the level of PTH and ALP increased significantly whereas calcium and phosphate of experimental group did not show significant rise. Conclusion: High intense interval training, possibly through increased PTH and protein factors, control bone mass density and volume and thus effecting bone metabolism. 3722 Microbiology Cloning and expression of Chlamydia trachomatis MOMP217 in E. coli Bitazar Razieh Bagheri Rezvan Salimi Ali Hajikhani Bahareh Qazvin University of Medical Sciences, Qazvin, Iran and Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran Tehran University of Medical Sciences, Tehran, Iran. Tehran, Iran. Shahid Beheshti University of Medical Sciences, Tehran, Iran 1 4 2015 22 130 38 46 30 05 2015 30 05 2015 Background: Chlamydia trachomatis is a silent, turbulent and unfortunately neglected bacterium which impairs human genital tract function. This Gram-negative obligate intracellular bacterium with unique life cycle and immunopathogenesis result in serious complications, such as Endometritis, pelvic inflammatory disease (PID), infertility, ability to ease HIV transmission and cofactor in human papilloma virus (HPV)-induced cervical neoplasia. To dominate these problems, vaccination is the best way of controlling the infection. Accordingly, MOMP has been implicated as a potential vaccine candidate through the years. Methods: In this study, MOMP217 gene was cloned in PET28b+ and expressed in E.coli and the protein expression confirmed by SDS-PAGE 10% Gel stained with Comassie Blue dye. Result: Beside the in silico experiments and the epitopic important regions in this conserved fragment, rMOMP217 may be a valuable peptide for immunization study. Conclusion: Conclusively, with approach to animal model study for evaluation of immune system response ability, this truncated protein may be introduced as a candidate in vaccine study. Absolutely, this attempt needs more experimental works. 3723 Internal Medicine C-reactive protein levels correlate with mortality in critically ill patients Hatami Samaneh Javad Mousavi Seyed Ali Iran University of Medical Sciences, Tehran, Iran 1 4 2015 22 130 47 52 30 05 2015 30 05 2015   Background: The aim of this study was to investigation the relationship between CRP level and mortality in ICU patients.   Methods: In this retrospective cross-sectional study 150 critically patients in a university hospital was evaluated. Age, gender, organ failure and CRP level was evaluated. Then correlation between mortality and CRP level was measured.   Results: The mean age of patients was years. %47.3 of patients were men and %52.7 of patients were women. Prevalence of mortality in this study was %64.7.   In this study was demonstrated that CRP concentration correlate with mortality (p<0.001).   Conclusion: In this study was revealed that CRP level was prognostic factor for mortality in critically ill patients.   2934 Physiology Contractile responses of thoracic aorta to hindlimb unloading in rat Soltani Hajar Hajebrahimi Zahra Arabian Maedeh Nasri Sima Aboutaleb Nahid Department of Biology, Payame Noor University, Tehran, Iran Astronautics Research Institute, Iranian Space Research Center, Tehran, Iran Astronautics Research Institute, Iranian Space Research Center, Tehran, Iran Department of Biology, Payame Noor University, Tehran, Iran Physiology Research Center Department, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran 1 4 2015 22 130 53 62 22 02 2014 24 12 2014  Background: Hindlimb Unloading (HLU) is used to simulate microgravity in rats and has been shown to decrease contractile response in the abdominal aorta. The aim of present study was to investigate the contractile responses of smooth muscle of thoracic aorta following acute and chronic microgravity treatment.  Methods: Male adult rats (Wistar) were subjected to HLU for 3 and 20 days to establish ground-based-model of microgravity. The thoracic aortas were dissected and suspended in Krebs-Henseleit solution less than 1 g resting tension. Concentration response curves to cumulative doses of phenylephrine (PHE) and KCL were obtained in endothelium-denuded rings from control and HLU groups. Statistical analysis was carried out using analysis of two-way ANOVA and post hoc Bonferroni test.   Results: Contractile response of 20-day HLU-treated tissues with phenylephrine and KCL were increased in endothelium-denuded rings versus control group (3.01±0.058 vs. 2.23±0.034 g at max (phenylephrine), HLU-20 vs. control 2.71±0.043 vs. 2.06±0.040 g at max (KCl), HLU-20 vs. control). There was no difference in vasoresponsiveness between control and 3-day HLU rings. Conclusion: Results of this study suggest that chronic HLU increase smooth muscle contracting in the thoracic aorta. It is proposed that these effects may be related to the fluid shifts in microgravity condition. Briefly, studies of vascular function are of particular importance in elucidating the mechanisms underlying vascular adaptation to microgravity and its gravity based countermeasure