Iran University of Medical Sciences
Razi Journal of Medical Sciences
2228-7043
2228-7051
22
133
2015
7
1
Response of interleukin 6, necrosis factor alpha and cortisol to intensive exercise in athlete’s women
1
7
FA
ba.tartibian@gmail.com
Y
N
N
Background: Studies show that changes in inflammatory cytokines
associated with inflammatory, hormonal changes in women has been less studied,
especially in female athletes. So aim of this research is the investigation of
Response of interleukin 6, necrosis factor alpha and cortisol to intensive
exercise in athlete’s women.
Methods: Among the volunteers, 15 subjects allowed to
participate in the study and then participated in Alstad exercise test
(gradient: 15%, Speed: 8 mph, time: 16 minutes). Blood sample were taken in
three stages: pre exercise test, immediately and 1 hour after (recovery) of
exercise test.
Results: cortisol, IL-6 and TNF-α levels were significantly
increased at all stages exercise training (At all stages: p=0.001). CRP levels
did not significantly change immediately after exercise, but significantly
decreased 1 hour after exercise (p=0.04).
Conclusion: intensive exercise increases inflammatory cytokines and
cortisol levels in athlete’s women. So it seems that during the intense
exercise athletes female are confronted with injuries of inflammatory markers
(cytokines and inflammatory hormones).
Interlukin-6, Necrosis factor alpha, Cortisol, Athlete’s women
http://rjms.iums.ac.ir/article-1-3893-en.html
http://rjms.iums.ac.ir/article-1-3893-en.pdf
Iran University of Medical Sciences
Razi Journal of Medical Sciences
2228-7043
2228-7051
22
133
2015
7
1
Evaluating the expression of putative stem cell markers ALDH1 and CD133 in melanoma cell lines A375 and D10
8
15
FA
N
N
N
N
N
madjd.z@iums.ac.ir
Y
Background: The cancer stem cells (CSCs) are involved in invasion
and metastasis of melanoma, the most lethal and aggressive form of skin cancer.
In the present study, the expression of putative stem cell markers CD133 and
ALDH1 were evaluated in melanoma cell lines and then the cells sorted based on
the expression of these markers.
Methods: In the present study expression of CD133 and ALDH1 was
evaluated in A375 and D10 cell lines using flow cytometry. Then, selected cell
line was sorted up on the expression of selected stem cell marker into positive
and negative populations.
Results: Our results indicated that ALDH1 marker expressed in
55.71 ± 7.88% and 49.88 ± 6.43% in A375 and D10 cell lines, respectively, while
the CD133 only expressed in D10 cells in 26.76 ± 2.83%. Cell sorting was
performed according to ALDH1 expression in both cell lines and CD133 expression
only in D10 cells. The purity of isolated cells was high according to CD133
expression (90% <), whereas the purity of ALDH1+ and ALDH1- populations was
very low.
Conclusion: Findings of this study showed that cell sorting based
on stem cell marker CD133 was suitable in D10 cells, therefore this marker
could be a reliable marker for isolating of CSCs in this cell line.
Melanoma, Stem cell marker, CD133, ALDH1, A375, D10
http://rjms.iums.ac.ir/article-1-3894-en.html
http://rjms.iums.ac.ir/article-1-3894-en.pdf
Iran University of Medical Sciences
Razi Journal of Medical Sciences
2228-7043
2228-7051
22
133
2015
7
1
Effects of Caffeine, L-Carnitine and Their Combination on 400m Freestyle Performance in Adolescent Female Swimmers
16
26
FA
mitra3291@yahoo.com
Y
N
N
N
Background: The study aim was to examine the effect of caffeine, L-carnitine, and their combination on lactate blood level, and exercise time during a 400m freestyle swimming trial.
Methods: In a double-blind randomly designed study, 16 Karaj female freestyle swimmers aged 13.87±1.14 yr, BMI 21.69±4.34 kg/m2, height 160.87±58.75cm, weight 56.31±1.31kg were recruited. The trial activities included completing maximal 400m freestyle. The activity was performed half an hour after taking caffeine (3 mg/kg body weight) or L-carnitine (1.5g), caffeine + L-carnitine (3 mg/kg + 1.5 g), or placebo on 4 similar separate occasions. Blood lactate was measured 1 and 10 min after exercise.
Results: Repeated measures ANOVA indicated that no significant difference was observed for performance in all groups (p≥0.05), whereas significant differences were observed in lactate accumulation in 1 min after trial (p<0.05).
Conclusion: These findings showed that consuming caffeine, L-carnitine and their combination has no meaningful effect on performance. However, it seems that taking caffeine alone or with L-carnitine result would result in more lactate accumulation in 1 min after exercise.
Caffeine, L-carnitine, Lactic acid, Swimming record, Adolescent female swimmers
http://rjms.iums.ac.ir/article-1-3895-en.html
http://rjms.iums.ac.ir/article-1-3895-en.pdf
Iran University of Medical Sciences
Razi Journal of Medical Sciences
2228-7043
2228-7051
22
133
2015
7
1
Single injection of platelet-rich plasma in knee osteoarthritis: a pilot study
27
34
FA
N
elaheh.mianehsaz@gmail.com
Y
N
N
N
N
Background: Platelet Rich Plasma (PRP) contains Growth Factors
(GFs) and antiinflammatory agents with possibility of application in
regeneration of damaged cartilage in the treatment of osteoarthritis (OA). In
the most trials, three injections of PRP have been done but in this research we
assessed the efficacy and safety of single injection of PRP in the management
of OA.
Methods: In this single-center, uncontrolled, prospective
preliminary study, 10 patients with knee OA who met the study criteria received
single PRP injections. Outcome measures included the Visual Analog Scale (VAS),
Knee Injury, Osteoarthritis Outcome (KOOS) questionnaire and 20 meters walk
test at pre injection visit, 3 week, 2 and 6 month follow up visits.
Results: There were no adverse events. The study demonstrated
significant improvements in VAS, 20 meters walk test and some KOOS scores,
including pain relief and better function in daily living (ADL) but some
subscale such as symptoms relief and sport activities and quality of life did
not significantly improved.
Conclusion: The study showed positive results of single PRP
injection and safety profile. Therefore, it could potentially be used to
larger, blinded, and randomized clinical trial to determine if PRP is more
effective than other treatments of knee OA or not.
Platelet-Rich Plasma, Knee, Osteoarthritis, Intraarticular injections
http://rjms.iums.ac.ir/article-1-3896-en.html
http://rjms.iums.ac.ir/article-1-3896-en.pdf
Iran University of Medical Sciences
Razi Journal of Medical Sciences
2228-7043
2228-7051
22
133
2015
7
1
Relation of serum Vitamin D level and pulmonary punction in COPD patients
35
40
FA
N
N
szrezae@yahoo.com
Y
N
N
Background: The aim of this study was to determine the mean of the
serum level of vitamin D in chronic obstructive pulmonary disease (COPD)
patients referred to Rasoul-E-Akram Hospital, Tehran, Iran in 2010-11.
Methods: In this sectional study 56 patients with COPD were
recruited. The COPD condition was confirmed in these patients following
spirometry. Serum vitamin D level of all patients was measured and categorized
according to the 5 stages of 0,1,2,3, and 4 suggested by Global Initiative on
Obstructive Lung Disease (GOLD). The data were analyzed using SPSS v. 18.
Results: The mean age of patients was 68.1 ± 10.3. About%76 of
patients were men. The study indicated that%85.1 of the patients were suffering
from vitamin D deficiency with the level less than 30 mg /dl. Also, it was
indicated that there is a direct and linear relation between serum vitamin D
level and forced expiratory volume in one second (FEV1) volume(p= 0.03), (B=
0.4).
Conclusion: The study demonstrated that vitamin D deficiency in
COPD patients is highly prevalent. Thus, diagnostic examinations of vitamin D
deficiency and on time treatment can prevent from many related problems in
these patients.
Serum vitamin D level, COPD, Spirometry
http://rjms.iums.ac.ir/article-1-3897-en.html
http://rjms.iums.ac.ir/article-1-3897-en.pdf
Iran University of Medical Sciences
Razi Journal of Medical Sciences
2228-7043
2228-7051
22
133
2015
7
1
Lived experience of Iranian women with HIV/AIDS and acknowledgment of their social rights: a phenomenological hermeneutical study
41
50
FA
N
N
naserirad@ut.ac.ir
Y
N
N
Background: In Iran, there has been a considerable increase in the
number of women infected with the human immunodeficiency virus (HIV). Within a
socio-political context, social rights inequality of HIV positivity is still a
prevailing concern among women. Little is known about the understanding of how
Iranian women living with HIV, live their social rights experience. The aim of
this study was to examine lived experience of these women against their social
rights.
Methods: In this qualitative study, a phenomenological–hermeneutic
framework was adopted. We conducted narrative interviews with sixteen women. A
purposeful sample of Iranian women was chosen.
Results: Five themes were identified to understand the
experience of social rights in women: 1) A state of profound suffering 2) lack
support 3) Insecurity in a social-economic life 4) Any respecting and 5)
Exerting oneself to keeping. For these women, social rights by their HIV status
meant: Living the ambivalence of a process of equality/inequality.
Conclusion: Health professionals have to broaden their role and
work on individual, interpersonal and inter-organizational levels. Mobilization
of actors from different sectors would facilitate the implementation of
pertinent and opportune interventions.
Lived Experience, HIV/AIDS, Hermeneutic phenomenology, Social rights, Women
http://rjms.iums.ac.ir/article-1-3898-en.html
http://rjms.iums.ac.ir/article-1-3898-en.pdf
Iran University of Medical Sciences
Razi Journal of Medical Sciences
2228-7043
2228-7051
22
133
2015
7
1
The effect of estrogen on p53 protein in T47D breast cancer cell line
51
58
FA
Robab.Sheikhpour@iauyazd.ac.ir
Y
N
Background: Breast cancer is one of the most common cancers in women. Nearly 30% of breast cancers are hormone-dependent, and these hormones comprising estrogens influence progression of breast cancers. It is now widely recognized that p53 may be the most frequently mutated protein in breast cancer. High levels of p53 protein are a common feature of many human malignant cancers. Given that, T47D cell line is estrogen and progesterone receptor positive and p53 protein is one of the most important tumor suppressor genes. This article examines the effect of estrogen on p53 protein in T47D.
Methods: The human breast cancer T47D cell line were cultured in 25cm2 flasks in DMEM medium supplemented with fetal bovine serum (FBS) and treated with different concentrations (3, 6, and 9 nmol) of estrogen. The levels of proteins were measured by western blot method. Gene tool software and One Way ANOVA were used for statistical analysis.
Results: Comparison of p53 levels in T47D cell line showed that cells that were exposed to 3, 6, and9 nmol of estrogen treatment had higher concentration of p53 than control (p<0.001).
Conclusion: The results showed that estrogen can strongly increase p53 protein concentration in T47D cell line. Therefore, it seems that estrogen can cause protein over expression and accumulation in T47D cell line.
p53 protein, T47D, Estrogen treatment
http://rjms.iums.ac.ir/article-1-3899-en.html
http://rjms.iums.ac.ir/article-1-3899-en.pdf
Iran University of Medical Sciences
Razi Journal of Medical Sciences
2228-7043
2228-7051
22
133
2015
7
1
Electroencephalography in children with simple, complex, and recurrent febrile seizures
59
63
FA
N
z_kalbassi@yahoo.com
Y
Background: Febrile seizures (FS) occur in 5% of children with 6 to 60 months ages. Many investigations are being performed, one of them is EEG. This study was done to determine EEG in recurrent simple and complex febrile seizures.
Methods: In this descriptive, cross sectional study, we evaluated 86 patients with recurrent and complex febrile seizure admitted to neurology ward in Ali-Asghar training children hospital between 2008 and 2012. Data were recorded on age, gender, type of seizure (focal, generalized), and family history of febrile seizure, epilepsy, and EEG results.
Results: Eighty six patients had EEG. Mean (SD) age was 24.27±15.28 months. Forty three (50%) cases were 6-18 month of age. Fifty three (62%) cases were male. Thirty three (38.5%) patients had recurrent simple FS and 53 (61.5%) had complex FS. Twenty nine (37.7%) cases had family history of FS and 13(15%) cases had family history of epilepsy. Thirty six percent of cases had abnormal EEGs. Abnormal finding included slow waves (14%), sharp waves (16%) and spike waves (6%). There was significant relation between abnormal EEGs and age (p=0.04), though no meaningful relation with gender, type of seizures and family history of FS or epilepsy.
Conclusion: Seizures during a febrile illness are common in children under 5 years of age. Due to changes in EEG in children with simple, complex, and recurrent FS, performing EEG in these cases is recommended.
EEG, Febrile seizure
http://rjms.iums.ac.ir/article-1-3900-en.html
http://rjms.iums.ac.ir/article-1-3900-en.pdf
Iran University of Medical Sciences
Razi Journal of Medical Sciences
2228-7043
2228-7051
22
133
2015
7
1
Detection of Oseltamivir Resistant Influenza A/H3N2 viruses by Real-time RT-PCR
64
69
FA
N
N
N
yavarian@tums.ac.ir
Y
Background: Currently, with increasing risk of influenza A virus
epidemics, a lot of studies have been performed. Oseltamivir or Tamiflu (the
neuraminidase (NA) inhibitor) is one of the effective drugs for preventing and
treatment of these viruses. The H274Y mutation is from the most important drug
resistant factors in influenza A viruses. The aim of this study was detection
of Oseltamivir resistant influenza A/H3N2 viruses by 274 position inspection
using Real-time RT-PCR.
Methods: Initially, specific primers and probs for detection of
sensitive and resistant A/H3N2 viruses were designed. The Real-time RT-PCR
assay was performed to detect mutation in 274 position of NA gene.
Results: Of 50 A/H3N2 specimens, all were negative for H274Y
mutation and no resistant viruses were selected.
Conclusion: Quick and accurate recognition of drug resistant mutants
is necessary for effective treatment strategies. Real-time RT-PCR assay is a
rapid operational test which could be performed in the laboratories for
detection of influenza viruses resistant to NA inhibitor.
Influenza A/H3N2 virus, Real-time RT-PCR, Drug resistance
http://rjms.iums.ac.ir/article-1-3901-en.html
http://rjms.iums.ac.ir/article-1-3901-en.pdf
Iran University of Medical Sciences
Razi Journal of Medical Sciences
2228-7043
2228-7051
22
133
2015
7
1
The study of immunoglobulin A, G and cortisol serum response in two consecutive soccer match and vitamin C supplements
70
79
FA
Shirvani122@yahoo.com
Y
N
Background: Regarding importance of anti-oxidative nutrition in
recovery period especially in relation with strengthening the immune system,
the aim of this study was to investigate the effect two consecutive soccer
matches with vitamin C supplementation during recovery on serum immunoglobulin
and cortisol in collegiate male footballers.
Methods: A sampling of 36 soccer footballers from football
leagues first division of Iranian universities were selected and randomly
divided in to three groups. supplementation group (SG) to perform two
consecutive football games and receive one-liter beverage containing 500 mg
vitamin C, placebo group (PG) also performed two consecutive football games and
received the same amount of
aspartame-containing beverage, while the control group (CG) did not receive any
intervention. Every football match consists of two 45- minute halves with 15
minute rest time in between and was held the interval of less than 48 hours of
the next contest. Supplements were consumed in period 1 hour after the second
games. Blood samples were collected before and one hour after second games for
measurement measuring of the relevant indicators items. The paired and
independent t-test was used to assess significant within and between groups to
analyze the results.
Results: As can be seen in the table, the changes within the
groups of IgA and cortisol (p=0.023) is significant (p=0.04) in the placebo
group.
Conclusion: Overall, it seems appears that vitamin C
supplementation in the form of effervescent tablets in during recovery from two consecutive soccer games can
moderated change in serum immunoglobulin A, G and cortisol levels in college football
players and may reduce the incidence of URTI in these
players.
Soccer, Muscle Fatigue, Immunoglobulin A and G, Serum Cortisol, Vitamin C
http://rjms.iums.ac.ir/article-1-3902-en.html
http://rjms.iums.ac.ir/article-1-3902-en.pdf
Iran University of Medical Sciences
Razi Journal of Medical Sciences
2228-7043
2228-7051
22
133
2015
7
1
The effects of barley grain (Hordeum Vulgare L.) during pregnancy on development and heart histopathological changes of embryos of diabetic rats
80
88
FA
N
N
minaezb@sina.tums.ac.ir
Y
N
N
N
N
Background: Diabetes mellitus in pregnancy can cause heart
histological changes. This study was done to evaluate the effect of barly grain
(Hordeum Vulgar L.) consumption during pregnancy in diabetic rats on
heart histological altrations of offsprings.
Methods: In this experimental study ,60 adult female albino
rats, randomly allocated into four groups including: healthy group with regular
meals consumption as control, healthy which consumed barley( 10 grams per each
rat per daily), diabetic with regular meals consumption and diabetic group
which consumed barley( 10 grams per each rat per daily). diabetes was induced
by intraperitoneal injection of 45 mg/kg/bw of streptozotocin. After
confirmation of pregnancy by observing the vaginal plug on 21th days, the dams
were anesthetized and embryos were removed. Crown rump length and weight of
embryos wre recorded.after heart tissue processing, sections with 5 micrometer
thickness were stained with H;E method.
Results: Cardiomyocytes
from change was
seen as hypertrophy,
interstitial plates and
Purkinje fibers reduction, inflammation
and congestion of
vessels were observed in embryos of diabeticts group. These tissues
alterations significantly reduced in the embryos of diabetic group
which consumed barley.
The crown rump length of embryos significantly reduced in diabetic
groupin comparision with controls. There was no many differences in crown rump
length of embryos between diabetic consumed barley and diabetic group. The
weight of embryos was non-significantly more in diabetic groups than controls.
The weight of embryos reducednon-significantly in diabetic plus barley
consumptionin comparision with controls.
Conclusion: The consumption of barley is
beneficial in reducing heart histological alterations in embryos of diabetic
rats.
Diabetes mellitus, Embryo, Heart, Barley grain, Rat
http://rjms.iums.ac.ir/article-1-3903-en.html
http://rjms.iums.ac.ir/article-1-3903-en.pdf