@article{ author = {Seif, Farhad and Khoshmirsafa, Majid and Moosavi, Mohammad and Roudbary, Maryam and Khajoei, Sholeh and Nikfar, Golnaz and Samei, Azam and Bahar, Mohammadali and Hashemzadeh-Chaleshtori, Morteza and Shirzad, Hedayatollah}, title = {Evaluating the expression of IL-17 and IL-23R genes in Peripheral Blood Mononuclear cells in Rheumatoid Arthritis patients}, abstract ={Background: Rheumatoid arthritis (RA) is an autoimmune disease caused by accumulation of numerous inflammatory cells in the joints and secretion of various cytokines leading to cartilage and bone damage. IL-17 and IL-23 are inflammatory cytokines that their definite role has not been clearly distinguished in RA pathogenesis. Therefore, this study aimed to investigate the expression and association of IL-17 and IL-23R in Peripheral Blood Mononuclear cells (PBMCs) in RA patients. Methods: This study was case-control. We gathered peripheral blood from 37 patients with RA and the same number of healthy individuals as a control group. In brief, PBMCs were isolated by Ficoll centrifugation. After RNA extraction and cDNA synthesis, IL-17 and IL-23R expression mRNA levels were determined in PBMCs by real-time PCR technique and Taqman probe method. Results: The mean±standard deviation of the ages in patient group was 46.86±1.328 yr. and in controls was 44.73±1.392 yr. The expression of IL-17 was increased in RA patients in comparison to healthy controls (P= 0.002). Whereas, after comparison of IL-23R expression in patient and healthy groups, no significant difference was observed (P = 0.22). Conclusion: In this study, upregulated expression of IL-17 implicated the important role of this cytokine in RA pathogenesis. Therefore, novel therapeutic and more effective strategies can be suggested by further investigations to specifically inhibit IL-17 using monoclonal antibodies (biologic drugs).}, Keywords = {Rheumatoid Arthritis, Peripheral Blood Mononuclear cells, IL-17, IL-23R}, volume = {25}, Number = {5}, pages = {1-8}, publisher = {Iran University of Medical Sciences}, url = {http://rjms.iums.ac.ir/article-1-5147-en.html}, eprint = {http://rjms.iums.ac.ir/article-1-5147-en.pdf}, journal = {Razi Journal of Medical Sciences}, issn = {2228-7043}, eissn = {2228-7051}, year = {2018} } @article{ author = {}, title = {Cloning and expression of AcAMP Aspergillus clavatus in E. coli and investigate its antibody titration in mice}, abstract ={Background: Antimicrobial peptides have been considered as the key to change in the current antibiotics. AcAMP, Antimicrobial peptide is one of them, which has been purified from the Aspergillus clavatus ES1 fungus, and its biochemical properties and characterization indicate that this peptide has unique properties. The aim of this study was to express the gene of AcAMP peptide in Escherichia coli (E. coli) and investigate its antibody titration in mice. Methods: In this experimental study, the AcAMP peptide-producing gene with BamHI and SalI enzymes was amplified from pUC57 containing gen using PCR and cloned to pET28a expression vector (+) and transformed into E. coli strain BL21 (DE3) in the next step. The expression of the AcAMP recombinant protein was induced by IPTG and purified by the affinity Ni-NTA chromatography. The recombinant protein was confirmed using Western blotting. Mice were intraperitoneally immunized with purified protein, and IgG titration was measured by ELISA method. Results: The recombinant pET28a (+) expression vector was confirmed by PCR and sequencing. The 9 kDa produced protein was confirmed by SDS-PAGE and Western blotting. Antibody production process, using indirect ELISA, indicates the increasing trend of antibodies titration, especially after the fourth injection. Conclusion: Purified recombinant AcAMP peptide and produced antibody can be used in antimicrobial research such as antimicrobial activity characterization of recombinant peptide and peptide identification in various applications.  }, Keywords = {Aspergillus clavatus, antimicrobial peptides, AcAMP peptide, laboratory mice, Antibody titration}, volume = {25}, Number = {5}, pages = {9-18}, publisher = {Iran University of Medical Sciences}, url = {http://rjms.iums.ac.ir/article-1-5203-en.html}, eprint = {http://rjms.iums.ac.ir/article-1-5203-en.pdf}, journal = {Razi Journal of Medical Sciences}, issn = {2228-7043}, eissn = {2228-7051}, year = {2018} } @article{ author = {Nejatbakhshsamimi, Leila and Fallahpour, Morteza and Khoshmirsafa, Majid and Baharloo, Rasool and Jorbozehdar, Paria and Mousavi, Seyed Ali Javad and Falak, Rez}, title = {Hormone replacement therapy: an effective approach in regulation of TH1-relatted responses in women suffering from allergic asthma}, abstract ={Background: The pattern of incidence of asthma varies with age and sex, as females suffer more than males. Some asthmatic women report premenstrual exacerbation of asthma symptoms as well as variation of its severity during pregnancy, thus it is believed that sex hormonal changes could affect asthma. Hormone Replacement Therapy (HRT) is a routine and accepted procedure which is used for treatment of several cases such as irregular periods, hirsutism, menopausal manifestations, acne, osteoporosis and amelioration of the symptoms in some autoimmune disease. HRT could reduce the magnitude of variations in estrogen and progesterone over the menstrual cycle. According to increased asthma prevalence among women than men and regarding to expression of estrogen and progesterone receptor on lung and immune cells, we aimed to determine the effects of 17β-estradiol (E2) and progesterone (P4) alone and in combination form on expression of T-bet and IFN-ɣ cytokine secretion, in correlation with Th1 cell subset of Peripheral Blood Mononuclear Cells (PBMC) (as crucial cells that could affect cytokines’ balance) in asthmatic patients versus non-asthmatic healthy controls. Methods: The diagnosis of asthma was confirmed on the basis of clinical symptoms and detection of allergen specific IgE. Then PBMCs were isolated and cultivated in 24-well plates in the presence or absence of 1% phytohemagglutinin (PHA), 10-8 M of estrogen and 10-6 M of progesterone, followed by mRNA isolation. After reverse transcription, real-time quantitative PCR was performed to evaluate the expression level of T-bet. We also measured the concentration of the related cytokine (IFN-ɣ) in supernatants by ELISA. Results: The expression of T-bet as well as secretion of IFN-ɣ which is a Th1 related cytokine was significantly increased when a combination of both hormones were applied in case group compared to controls [Median: 84.04 (IQR: 77.32-177) and Median: 71.52 (IQR: 68.85-84.04) pg/ml respectively], however, treatment with these hormones alone did not show any significant effects. Conclusion: We concluded that, treating PBMCs with estrogen and progesterone alone or in combination as an in vitro example of HRT, has stimulatory effect on Th1 cells’ behavior that may have a role in improving (sometimes worsening because of the complex role of CD8+T cells) of allergic asthma symptoms. It is crucial to clarify the effect of these hormones on differentiated T helper cell population, which requires more studies to understand the effect of sex hormones on allergic asthma.  }, Keywords = {Allergic asthma, PBMCs, Estradiol, Progesterone, Hormone Replacement Therapy}, volume = {25}, Number = {5}, pages = {19-28}, publisher = {Iran University of Medical Sciences}, url = {http://rjms.iums.ac.ir/article-1-5154-en.html}, eprint = {http://rjms.iums.ac.ir/article-1-5154-en.pdf}, journal = {Razi Journal of Medical Sciences}, issn = {2228-7043}, eissn = {2228-7051}, year = {2018} } @article{ author = {azadbakht, masoomeh and Yazdanbakhsh, kamran and moradi, asie}, title = {Effectiveness of cognitive rehabilitation on improving auditory and visual working memory in obsessive-compulsive disorder}, abstract ={Background: Regarding the prevalence of obsessive-compulsive disorder of all ages and its adverse effects in childhood and adolescence, it is absolutely necessary to review and evaluate new therapies with low complications and appropriate treatment effects. The purpose of the present study was to determine the effectiveness of cognitive rehabilitation on improving the auditory and visual working memory secondary school girl adolescents with obsessive-compulsive disorder. Methods: The present study was a semi experimental design with pretest-posttest design with control group. The population of the study included all girls suffering from obsessive-compulsive disorder in Koohdasht high schools in the academic year of 2011-2012. By random selection of four high schools and screening using Fava et al., A compulsory obsessive-compulsive inventory questionnaire, after interviewing them, 30 students who were selected to meet the criteria for entering and leaving the research were purposefully selected and they were randomly assigned to two groups. From both groups, Wechsler's active memory test was performed and a cognitive rehabilitation program was performed for the experimental group individually for 12 sessions. Results: After intervention, cognitive rehabilitation, auditory active memory (p<0.001) and active activity memory (p<0.001) increased in experimental group compared to control group. Conclusion: The results of this study showed that cognitive rehabilitation is effective in improving the working memory of patients with OCD. Therefore, it is suggested that, in addition to psychological interventions, cognitive rehabilitation interventions should be used as an integrated, applied and supportive therapeutic approach along with psychotherapy by specialists in this field.}, Keywords = {Cognitive rehabilitation, Obsessive-compulsive disorder, Visual and audible working memory}, volume = {25}, Number = {5}, pages = {29-37}, publisher = {Iran University of Medical Sciences}, url = {http://rjms.iums.ac.ir/article-1-5001-en.html}, eprint = {http://rjms.iums.ac.ir/article-1-5001-en.pdf}, journal = {Razi Journal of Medical Sciences}, issn = {2228-7043}, eissn = {2228-7051}, year = {2018} } @article{ author = {MoradiAhmar, Masoumeh and Azadi, Mehr}, title = {Effectiveness of the Solution Focused Group Intervention on Body Image after Mastectomy}, abstract ={Background: Women with breast cancer that have undergone mastectomy surgery will gradually become sensitive to their physical deficiency which may cause distortion in their body image. One of approaches that will improve their attitude is solution focused treatment. The purpose of this study is to determine the effect of short term solution focused group intervention on body image of women after mastectomy. Methods: The plan used in this investigation was a quasi-experimental type with pre-test and post-test and accompanied with control group. Participators were matched up based on the time passed from their breast surgery and chemotherapy and were randomly assigned to two groups of 15 for experiment and 15 for control. Both groups were obliged to answer the multidimensional body–self relations questionnaire (Cash, Winstead and Janda, 1987). Then the experimental group participated in short term solution focused group therapy for 6 weeks. Results: Results of analyzing covariance of data obtained from both groups before and after treatment intervention indicated significance of statistical tests (p<0.05). Conclusion: The current research showed that short term solution focused treatment improves body image of women that had undergone mastectomy significantly and has an efficient role in repairing damaged body image of these patients.  }, Keywords = {Short term solution focused group intervention, Body image, Mastectomy}, volume = {25}, Number = {5}, pages = {38-45}, publisher = {Iran University of Medical Sciences}, url = {http://rjms.iums.ac.ir/article-1-5015-en.html}, eprint = {http://rjms.iums.ac.ir/article-1-5015-en.pdf}, journal = {Razi Journal of Medical Sciences}, issn = {2228-7043}, eissn = {2228-7051}, year = {2018} } @article{ author = {Zaeemzadeh, Narges and Mottaghi, Azadeh and Mohamadzadeh, Neda and ziaiee, Saieedeh}, title = {The study of dietary intake of macronutrients in four phenotypes of polycystic ovary syndrome based on Rotterdam criteria}, abstract ={Background: Polycystic Ovary Syndrome (PCOS) is the most common endocrine disorder in reproductive-age women in Iran. Nutrition is considered as one of the probable affecting factors on the severity of PCOS. Till date, there is no study on dietary intakes of PCOS phenotypes, separately. Thus, this study sought to assess the dietary intake of macronutrients in four phenotypes of PCOS. Methods: This case-control study which was approved by the Medical Ethics Committee of Tarbiat Modares University was conducted by convenience sampling method on 182 patients eligible for the study. Subjects were classified according to the Rotterdam criteria: A (n=41), B (n=33), C (n=40), D (n=37) and control (without PCOS) (n=31). Assessment of dietary intake (Macronutrients), was carried out by valid-reliable 168-items food frequency questionnaire. Statistical analysis was performed using SPSS22 software and Kruskal-Wallis (KW) test followed by appropriate post hoc test, Mann-Whitney U (MW). Significant P-value was considered 0.05 for KW test and 0.005 for MW (based on Bonferroni correction). Results: Total fat intake in subgroups A, B and C was lower than controls (P<0.001). Phenotype A had a lower intake of PUFA and MUFA (P<0.005), and a higher intake of trans fatty acids (P<0.001) compared with controls. All PCOS phenotypes had a higher consumption of carbohydrates and a lower fiber consumption than controls (P<0.001). There was no significant difference between different phenotypes of PCOS with each other and with the control group in terms of saturated fat, protein and soluble fiber (P> 0.05). Conclusion: It is suggested that the complications associated with PCOS can be greatly reduced by changing in nutritional situation of these patients. It is a major step towards improving the status of these patients.  }, Keywords = {Phenotypes, PCOS, Macronutrient, Nutrition}, volume = {25}, Number = {5}, pages = {46-56}, publisher = {Iran University of Medical Sciences}, url = {http://rjms.iums.ac.ir/article-1-5029-en.html}, eprint = {http://rjms.iums.ac.ir/article-1-5029-en.pdf}, journal = {Razi Journal of Medical Sciences}, issn = {2228-7043}, eissn = {2228-7051}, year = {2018} } @article{ author = {Rohani, Soheyla and Hajighasemi, Fatemeh and Sefid, Fatemeh}, title = {Determination of spatial epitopes on human immunoglobulin light chain by computational immunology}, abstract ={Background: Immunoglobulins are a group of proteins that have important role in defense against microorganisms. Immunoglobulins consist of heavy and light chains. In human, immunoglobulin light chain comprises of two isotypes: Kappa (K) and lambda (λ) based on amino acid differences in carboxylic end of their constant region. Marked changes in the K to λ ratio can happen in monoclonal expansion of neoblastic B cells or HIV infection in neonatal periods. Highly sensitive and specific anti-light chain MAbs have clinical importance in the diagnosis and immunotherapy of patients with B-cell immunoproliferative diseases. Thus, precise determination of specific epitopes in light chains is very important. Computational immunology uses the computational data for more accurate diagnosis of diseases. This study describes determination of conformational epitopes in constant region of human immunoglobulin light chain by computational immunology. Methods: The amino acid residue and third structure of reference human immunoglobulin G light chain was found in PDB database. The second immunoglobulin G structure was defined by Phyre 2 software. Conformational epitopes of the immunoglobulin light chain were specified by CEP software. Results: In this study five conformational epitopes located on constant domain of human immunoglobulin light chain were determined by CEP software. These conformational epitopes were located in 100-214 amino acid sequences of light chains. Conclusion: In this study a number of conformational epitopes located on constant domain of human immunoglobulin light chain were determined. These epitopes are valuable tools for generating specific monoclonal anti-immunoglobulin light chain antibodies and might have possible implication in production of specific diagnostic kits for human immunoglobulin light chain, monitoring of monoclonal light chain diseases, treatment of related B cell tumors, epitope mapping of immunoglobulin light chain and evolutionary studies.  }, Keywords = {Human Immunoglobulins, Light chains, Conformational epitope, Computational immunology}, volume = {25}, Number = {5}, pages = {57-64}, publisher = {Iran University of Medical Sciences}, url = {http://rjms.iums.ac.ir/article-1-5153-en.html}, eprint = {http://rjms.iums.ac.ir/article-1-5153-en.pdf}, journal = {Razi Journal of Medical Sciences}, issn = {2228-7043}, eissn = {2228-7051}, year = {2018} } @article{ author = {yavari, mahsa and moosavai, alijavad and janani, leila and feyzi, zahra and vafa, mohamadrez}, title = {Effect of supplementation of vitamin D, C and E on the lung spirometry and plethysmography in Idiopathic Pulmonary Fibrosis patients}, abstract ={Background: Idiopathic pulmonary fibrosis is one of the most important chronic respiratory diseases. One of the causes of its occurrence and exacerbation is imbalance in the antioxidant and inflammatory systems. The aim of this study was to evaluate the effect of supplementation of these vitamins on functional lung tests in adults with pulmonary fibrosis. Methods: 33 patients participated in this quazi clinical trial for 3 months and supplemented with E, C and D vitamins with 200 IU, 250 mg and 50000IU doses, respectively. At the beginning and end of study anthropometric measurements and Body Mas Index (BMI), 24-hour recall, physical activity, sun exposure and Saint George questionnaires were investigated. Moreover, lung function factors were measured by using spirometry and plethysmography. Data were analyzed by SPSS version 21 and paired t-test statistical test. Results: The results obtained from spirometry and plethysmography tests represent a significant increase in percent of FEV1 (p= 0.016), IRV (p= 0.001), RV (p= 0.002) and TLC (p= 0.003). But there was no significant difference in percentage of FVC, VC, FEV1/FVC and ERV (p> 0.05). The rates of respiratory function have significant improvement according to the Saint George questionnaire. Also, there was no significant difference in confounding variables. Conclusion: It seems that taking 3 months of D, C and E vitamins in patients with lung fibrosis has a significant effect on respiratory function.  }, Keywords = {pulmonary fibrosis, vitamins C, D, E , spirometry, plethysmography}, volume = {25}, Number = {5}, pages = {65-72}, publisher = {Iran University of Medical Sciences}, url = {http://rjms.iums.ac.ir/article-1-5158-en.html}, eprint = {http://rjms.iums.ac.ir/article-1-5158-en.pdf}, journal = {Razi Journal of Medical Sciences}, issn = {2228-7043}, eissn = {2228-7051}, year = {2018} } @article{ author = {Razmjou, Elham and Meamar, Ahmad Reza and Motevalian, Abbas and Akhlaghi, Lameh}, title = {Prevalence of intestinal parasites in individuals referred to Milad hospital, Tehran, Iran}, abstract ={Background: Intestinal parasitic infection is still one of the major health concerns in the world and particularly in Iran. Due to the lack of a clear picture of the distribution of intestinal parasitic infection in Tehran, this study was conducted to determine the prevalence of intestinal parasites in patients referred to the Milad Hospital in Tehran. Methods: In this descriptive cross-sectional study, three time-separated fecal samples were collected from 448 consented patients through an easy sampling from April ‎to December 2011. The samples were examined by three methods, direct smear, formalin-ether concentration, and culture in horse serum ‎medium. Associations between prevalence of intestinal parasiticinfection ‎ and demographic characteristics of individuals, and reason for referral were evaluated. Results: The prevalence of enteric parasites was 32.4%. One hundred forty-five individuals were infected with at least one of the species of intestinal protozoon including; 134 (29.9%) Blastocystis sp., 14 (3.1%) Entamoeba coli, 6 (1.3%) Giardia lamblia, 5 (1.1%) Endolimax nana, 3 (0.7%), Entamoeba complex, 3 (0.7%) Iodamoeba butschlii, 2 (0.4%) Dientamoeba fragilis, 1 (0.2%) Trichomonas hominis, intestinal helminth infection including; 1 (0.2%) Trichostrongylus sp. and 1 (0.2%) Hymenolepis nana. Statistical analysis showed that chance of infection with intestinal parasites increased with age (p=0.003). There was no correlation between prevalence of intestinal parasitic infection and gender or reason for referral. Conclusion: This study revealed an overall prevalence of 32.4% intestinal parasitic infection among patients referred to the Milad Hospital, a community center in Tehran. The enteric protozoan parasite of Blastocystis sp. infection had the highest prevalence, being 29.9% as most of the species detected are believed to be transmitted through cysts along with water and food. Improving public education, sanitation and hygiene standards is expected to reduce transmission of intestinal parasites in this population.}, Keywords = {Prevalence, Intestinal protozoon, intestinal helminth, Tehran}, volume = {25}, Number = {5}, pages = {73-82}, publisher = {Iran University of Medical Sciences}, url = {http://rjms.iums.ac.ir/article-1-5230-en.html}, eprint = {http://rjms.iums.ac.ir/article-1-5230-en.pdf}, journal = {Razi Journal of Medical Sciences}, issn = {2228-7043}, eissn = {2228-7051}, year = {2018} } @article{ author = {}, title = {The effect of low back pain reduction school program on quality of life among health network staffs}, abstract ={Background: The most common and most costly occupational diseases are low back pain. In the field of low back pain, several programs can reduce the severity of pain. Low back pain training programs are a short-term program and include training, assessment and skill improvement programs. The purpose of this study was to evaluate the effect of this program on the quality of life of workers with low back pain in Lamer Health Network. Methods: This study was an educational intervention that included 32 employees with chronic low back pain in the intervention group and 29 employees in the control group. The control group did not receive any training. On the other hand, the intervention group was subjected to a controlled interventional program. The quality of life of these patients was evaluated at the beginning of the study and after 6 months by the SF-36 questionnaire. At the end, the results before and after the intervention were compared between the two groups. Results: This study showed that low back pain reduction program is an effective treatment for improving the quality of life of patients with low the effect of low back pain education curriculum on quality of life of health network staff back pain. The staff treated with this program had a higher score on the indicators of "physical condition", "physical performance," and "general health", which reflects the higher quality of life of patients in terms of these items in the intervention group. There was no significant difference between the two groups in other indices. Conclusion: Regarding the high prevalence of low back pain in health care workers, low back pain education programs, as an effective intervention for reducing back pain, can improve the quality of life of workers with chronic low back pain.    }, Keywords = {Educational programs, Teaching management, Low back pain, Quality of life, Staff}, volume = {25}, Number = {5}, pages = {83-89}, publisher = {Iran University of Medical Sciences}, url = {http://rjms.iums.ac.ir/article-1-5323-en.html}, eprint = {http://rjms.iums.ac.ir/article-1-5323-en.pdf}, journal = {Razi Journal of Medical Sciences}, issn = {2228-7043}, eissn = {2228-7051}, year = {2018} }