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Iran University of Medical Sciences
Razi Journal of Medical Sciences
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article
Production of recombinant human growth hormone by eukaryotic CHO cell and measurement of its biological activity by gene reporter assay
Marzeieh Rezaei
1
Seyyed Hamid Zarkesh-Esfahani
2
University of Isfahan
University of Isfahan
Background: Cultivated mammalian cells, because of their
capacity for proper protein folding, assembly and post–translational
modification, have become the dominant system for production of recombinant
proteins in clinical application. Therefore, the quality and efficacy of
protein can be superior when expressed in mammalian cells compared to other
hosts such as bacteria. Gene reporter systems have contributed greatly to the
study of eukaryotic gene expression and regulation. Although reporter genes
have played a significant role in numerous applications, both in vitro and in
vivo, they are most frequently used as indicators of transcriptional activity
in cells. Luciferase-reporter assays are widely used to monitor the cellular
events related to transduction and gene expression regulated by specific
cascades, such as PRL/Jak2/Stat5 pathway. Methods: In this study, recombinant human growth
hormone (rhGH) was produced in eukaryotic Chinese hamster ovary (CHO) cell and
production and concentration of rhGH verified by ELISA and western blotting.
Then, the biological activity of rhGH was assessed by a gene reporter assay
system (containing LHRE, TK promoter and Luc gene), using HEK 293 cells
transfected with GH receptor and response element for STAT-5 measuring
luciferase activity on a Berthold luminometer. Results: The date showed that rhGH could be produced by
eukaryotic host in good quantities as assessed by ELISA and western blotting.
The results of gene reporter assay showed that rhGH produced by CHO cells is
able to induce GH intracellular cell signaling. The rhGH produced by CHO cells
showed higher bioactivity when compared to commercial GH. Conclusion: rhGH could be produced in mammalian cell
lines at high levels with higher bioactivity. Gene reporter assay is a
sensitive, quantitative, rapid, easy, reproducible and safe system for
assessment of bioactivity of recombinant proteins such as rhGH.
http://rjms.iums.ac.ir/article-1-2383-en.pdf
Chinese hamster ovary (CHO)
Human growth hormone (hGH)
Bioassay
Gene reporter assay
ELISA.
per
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Assessing the effect of educational program based on small group on promoting knowledge and health literacy among women with type2 diabetes referring to selected hospitals affiliated to Tehran University of Medical Sciences
Azar Tol
1
Abolghasem Pourreza
2
Abass Rahimi Foroshani
3
Elahe Tavassoli
4
Tehran University of Medical Sciences
Tehran University of Medical Sciences
Tehran University of Medical Sciences
Shahid Beheshti University of Medical Sciences
Background: Health
Literacy is the capacity in which
individuals have to obtain, process, and understand basic health information
and services needed to make appropriate health decisions. Limited health
literacy can reduce adults’ ability to comprehend and adhere treatment plans.
This study was designed and implemented to assess effect of health education on
promoting knowledge and health literacy in women with type 2 diabetes. Methods: This randomize clinical trial study, was
conducted among 160 women with type 2 diabetes, in two experimental and control
groups. Tools of current study were a brief form of standard questionnaire
(Short Test of Functional Health Literacy in Adults, TOFHLA) and knowledge was
measured by self-administrated questionnaire. Intervention in a form of small
groups, was performed in 6 educational sessions (45-60minuts) in experimental
group. In order to analyzing data, SPSS16 software was used and independent t-
test, Kruskal-Wallis, Whitney U، Wilcoxon, Co-Variation, Chi-square were conducted. Results: Demographic variables of studied population in
two groups was similar before intervention (p>0.05). Differences between the
scores of Knowledge and Health Literacy in two groups, after and before
intervention, was totally different (after, p0/05). Conclusion: Study findings indicated that education with
small group's strategy in experimental group, in comparison with current
education is effective.
http://rjms.iums.ac.ir/article-1-2384-en.pdf
Education
Knowledge
Health Literacy
Type 2 diabetes.
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article
Association of age and work experience with work-related injuries in mining and mineral industries in Iran 2003 – 2011
Mashallah Aghilinejad
1
Jalil Kouhpayezade
2
Mohammad Kazem Noori
3
Majid Golabadi
4
Tehran University of Medical Sciences
Tehran University of Medical Sciences
Tehran University of Medical Sciences
Tehran University of Medical Sciences
Background: Based on estimations, each year about 250
million work-related injuries and many temporary or permanent disabilities
occur which most are preventable. Mining and metal industries are among
industries with high incidence of injuries in the world. The aim of this study
was assessment of incidence of and age and work experience characteristics in
work-related injuries in mining and mineral industries in Iran. Methods: In a
retrospective study data about severe non fatal and fatal work related injuries
gathered from accident registry system of the governmental Iranian mining and
mineral industries development & renovation organization (IMIDRO) between
2003 and 2011. Injury characteristics including injury types, injured body
parts, injury outcome and also age and work experience categorized. Incidence
rates and Injury characteristics assessed based on age and work experience
groups in injured workers. Results: 10032 work-related injuries and 197 deaths
were reported in the mentioned period. Mean age of injured workers was 33.9
(SD=8.4) years and mean work experience was 7.7 (SD=6.6) years. Age in 76
percent of cases was less than 40 years. 49.9% of them had work experience less
than 5 years. Most common accident type was trauma and impact (27%) followed by
falls (24.1%). Most common injured parts were lower limbs (33.3%) and upper
limbs (33%). Most common accident outcome was strain and sprain (42.4%). There
were some differences between age and work experience groups. Conclusion: Occupational injuries are more common in
younger and less experienced workers. High risk workers should be recognized
and suitable programs should be considered for them.
http://rjms.iums.ac.ir/article-1-2385-en.pdf
Occupational injuries
Age
Work experience
Incidence
Metal industries
Mining.
per
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article
Surgical tracheostomy versus percutaneous tracheostomy in the intensive care unit
Shahram Seyfi
1
Valliollah Hassani
2
Seyed Hamid-Reza Faiz
3
Shahrbanoo Latifi
4
Mohammad Reza Hajiesmaeili
5
Alireza Sedaghat
6
Reza Soleimanirad
7
Babol University of Medical Sciences
Tehran University of Medical Sciences
Tehran University of Medical Sciences
Babol University of Medical Sciences
Tehran University of Medical Sciences
Tehran University of Medical Sciences
Tehran University of Medical Sciences
Background: Prolonged
intubation and need to mechanical ventilation is an indication for
tracheostomy. This procedure can be done, surgical or percutaneous. In this
study, we compare surgical and percutaneous dilated tracheostomy. Methods: In this retrospective study, which was
conducted in ICU wards of Rasool Akram Hospital from Mehr 1387 to Mehr 1390. We
compared complication of tracheostomized patients in two groups. Results: In this study, early postoperative bleeding,
in 11 cases, 1case (4.2%) in PDT group and 10 cases (17.5%) in surgical group
was shown (with a significant difference). Surgical site infection in 1 case, 2
cases (7.4%) in PDT group and 1 case (4.2%) in surgical group was shown (with a
non-significant difference). Subcutaneous emphysema in 11 cases, 2 case (8.3%)
in PDT group and 1 case (1.7%) in surgical group with a significant difference,
was shown. Other complications such as tracheal posterior wall were not shown
in both groups. Conclusion: Because of lower complications rate and
bedside PDT doing, percutaneous tracheostomy is a safe and suitable alternative
for surgical tracheostomy.
http://rjms.iums.ac.ir/article-1-2386-en.pdf
Tracheostomy
Percutaneous
Surgery
Complication.
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article
Assessment of the relationship between psychological factors and impotency with quality of life of male patients following CABG
Said Pournaghash Tehrani
1
Soraya Etemadi
2
Tahereh Dehdari
3
Masood Gholamali Lavasani
4
Said Sadeghian
5
University of Tehran
University of Tehran
Tehran University of Medical Sciences
University of Tehran
Tehran University of Medical Sciences
Background: Given the increase in prevalence of Coronary
Artery Bypass Graft (CABG) surgery and the significant role of psychological
factors in prognosis and relapse of cardiac disease, the present study is
designed to determine the relationship between psychological factors or
impotency and quality of life of male patients following CABG. Methods: In this descriptive- analytical study 160
male patients were randomly selected four weeks after heart surgery and were
administered DASS-21, IIEF-5 and SF-36 to assess anxiety, depression and stress,
impotency and quality of life, respectively. Regression analyses (One-way
ANOVA) and Chi-square were performed to analyze the data. Results: Findings showed that the average age of
patients was 60 (±10) years. Among psychological variables the average score
for depression was significantly related to the average scores of quality of
life (p=0.02). Furthermore, 62.4% of patients had some degrees of depression
86.7% had some degrees of stress and 47.3% had anxiety to some degree. Also,
85.5% of patients displayed mild to severe levels of erectile dysfunction which
were significantly correlated with scores in anxiety (p=0.03) and depression
(p=0.001). Finally, diabetic patients had higher scores in depression and
stress compared to non diabetic patients. Conclusion: The findings
are discussed in the context of proposing interventional strategies for
improving psychological conditions of CABG patients.
http://rjms.iums.ac.ir/article-1-2387-en.pdf
CABG
Impotency
Anxiety
Depression
Stress.
per
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article
The effect of intensive activity and regular exercise with carbohydrate ingestion on cell-mediated immunity
Gholam Reza Jahani
1
Kobra Entezami
2
Hossan Haydari
3
Alireza Abkar
4
Zohre Mollasaeidi
5
Abhar branch, Islamic Azad University
Tehran University of Medical Sciences
Abhar branch, Islamic Azad University
Abhar branch, Islamic Azad University
Background: The purpose of this study was to evaluate
“the effect of intensive activity and regular exercise with carbohydrate
ingestion on cortizol, lymphocyte, monocyte, white blood cell, fast blood
suger, CD3, CD4, CD8 and CD4 to CD8 ratio, cell-mediated immunity”. Methods: This semi experimental, cross sectional and
double blind study performed to investigate the responses of cell-mediated
immunity after 12 weeks intensive activity and regular exercises. 39 sedentaryand
healthy studentswith same-life style, were selected .They did not use
cigarette, alcohol, supplementary and they had not infection diseases. Students
selected randomly and then divided into three groups: Glucose and train (TG)
23±2.1years, 73±8.2 Kg, 179±4.7Cmand with 22±2.5 BMI. Train (T),
23.5±1.8 years, 71.7±7.8 Kg, 176.4±3.6 Cmand 22±1.9 BMI. Control (C) 23.7±1.1
years, 72.4±6.6 Kg, 174.8±3.5 Cmand 22±2 BMI, they did endurance and speed
train for 12 weeks. TG group drank 2cc/Kg, glucose5% monohydrate & water
solution, in the middle of their train session. T group drank 150 – 200 ml
water. Bloods sample were withdrawn from antecubital vein after 14±2/hr fasting
and evaluated before, immediately and after 48/hr of train. As well as cellular
determined by immunology assay. For analyze in between groups ,one way ANOVA
with significan used
from modify method of green house-ghezer (GG), level
, were used. Results: There were not
any differences in T, TG and C group before 12 weeks exercise. The amounts of
cortizol in TG & T groups significantly increased after exercise, but there
was a lower increase in TG group. Results was significantly decreased after
48/hrs of train in before and after train parameters. The FBS in TG and T groups
had not any differences but in TG group / T group results was significantly
lower, after 48 hours. There was not any diffrences in amount of LYM after
train but after 48/hrs, it significantly increased in TG group. The amount of
WBC increased in after train but significantly decreased after 48/hr in TG
group. The CD4 significantly increased after train in TG group, and CD3
significantly increased after train and after 48/hrs in TG group. Monocytes,
ratio CD4/CD8 and CD8 amount had not any differences in TG and T groups. Conclusion: This research showed that intensive activity
and regular exercise with cabohydrat digestion induced increase some of immune
cells and cellular defence against infection disease that caused from intensive
exercises. Another finding of this investigation indicates that drinking a CHO
solution during exercise, improves performance. This study has practical
implications for those sports and drinking CHO solution during activity. Carbohydrates as a
factor can increase security against infection disease risk in recovery time
and after intensive exercise.
http://rjms.iums.ac.ir/article-1-2388-en.pdf
Combind training
Immunity system
Carbohydrate
Cortizol.
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Iran University of Medical Sciences
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article
Comparision of antifungal effect of Nystatin alone and in combination with nanosilver particles against candida species isolated from chronic candidal vaginitis
Shima Nozari
1
Fariba Haydari Kohan
2
Farzaneh Ahmadi
3
Mehrdad Asadi
4
Fariba Fallahi
5
Zaynab Ghasemi
6
Mehraban Falahati
7
Hormozgan University of Medical Sciences
Tehran University of Medical Sciences
Shahid Beheshti Medical University
Tehran University of Medical Sciences
Tehran University of Medical Sciences
Tehran University of Medical Sciences
Tehran University of Medical Sciences
Background: Nystatin is a polyene with antifungal effect which is used in treatment of cutaneous
and mucocutaneous candidiasis in diverse forms. Increasing its utilization in
recent years has led to predominant increasing of resistances. Nowadays for
increasing of antifungal effect and decreasing of resistance and side effects
of drugs they are used in combination with each other. So we decided to
investigate antifungal effect of Nystatin in combination with nanosilver
particles. Methods: This was an experimental study which has been
accomplished on 30 samples of isolated candida species from patients effected
to chronic candidal vaginitis. In this study the antifungal effects of Nystatin
and silver nanoparticles each of them alone and in combination with each other by
microdilution broth, were examined. Findings were described on the base of
logestic regression and man-vitni exam. Results: Findings suggested that Nystatin was able to
inhibit the growth of candida species at an expanded range of concentration
between 16-128 microgeram per milliliter. As well antifungal activity of
Nystatin with silver nanoparticles was increased in comparision with using
Nystatin alone. Conclusion: Introduce of nanosilver in drug formation of
Nystatin can be useful in treatment of chronic vaginal candidiasis.
http://rjms.iums.ac.ir/article-1-2389-en.pdf
Nystatin
Nanosilver
Antifungalactivity
Synergism
Candida Species.
per
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article
Comparative survey of youth educational needs for mental health promotion in marital life in two stages before and after marriage
Davood Pourmarzi
1
Shahnaz Rimaz
2
Effat al-sadat Merghati Khoii
3
Maryam Razi
4
Guilan University of Medical Sciences
Tehran University of Medical Sciences
Tehran University of Medical Sciences
Guilan University of Medical Sciences
Background: Educational programs for promotion of mental
health in marital life are necessary. Ignorance
of target group needs
diminishes effectiveness of efforts in educational programs. This research
aimed to report and compare mental health educational needs before and after
marriage. Methods: In a cohort study in 2011, by random stratified sampling 450 males and females were selected. These
were participated in pre-marital counseling in Hasheminezhad center in Tehran.
A researched-made questionnaire was used to measure rate of mental health
educational needs with 5 degree-Likert scale before and six months after marriage. Data
were analyzed by Independent t-test, paired t-test and Prioritization was done by calculating
mean rank. A P-value less than 0.05 have been considered as significant
different. Results: “How to keep romance alive in marital life”
topics was the first priority in both before and after marriage. Rates
of mental health educational needs in both stages were more than average (3
degree). After marriage rate of needs was increased but it was not significant.
Women in both stages report more rate of educational needs than men but this
difference wasn’t significant. Conclusion: Designing mental health promotion educational
programs based on people needs are necessary and these programs should be
performed in different stages of life.
http://rjms.iums.ac.ir/article-1-2390-en.pdf
Education
Mental health
Marital life.
per
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article
Determination of the frequency of prophylaxis usage against deep-vein thrombosis in hospitalized patients admitted in an educational hospital
Morteza Khavaninzadeh
1
Seyyed Saber Mousavi Vaeezi
2
Ehsan Ramezaniyan Nik
3
Kobra Reza Gharebagh
4
Tehran University of Medical Sciences
Tehran University of Medical Sciences
Background: Misdiagnosis and treatment of Deep Vein
Thrombosis (DVT) and Pulmonary Thromboembolism (PTE) usually cause some
problems and also lengthen the hospitalization period and treatment costs. Prophylaxis often reduces the risk of DVT and
following PTE, therefore in this study we aimed to determine the frequency of
using Prophylaxis to prevent DVTs in Rasoul Akram hospital in Tehran, Iran. Methods: In this Retrospective descriptive study, 202
participants were chosen from the patients admitted for surgery in the surgery
unites and randomly stratified and classified. Information was extracted for these participants, based on variables and
the objectives of this study and after that the frequency of using prophylaxis
to prevent DVT was calculated by using descriptive analysis tool. Results: Our study consisted of 105 male (%52) and 97
female (%48) patients in which 62(%30.7) were from surgery unite, 52(%25.7)
orthopedic , 36( %17.8) Gynecology, 33(%16.3) neurosurgery and 19(%9.4) urology. 114(%56.43) of
participants received prophylaxis versus 88 (%43.56) the patients did not
receive prophylaxis. 13 in high risk groups, 10 in the moderate risk group and
91 were in low-risk group. Conclusion: In our study, the correct usage of
prophylaxis was in %24.3 of incidents, which was consistent with previous
studies. In older populations who are subject to more risks in treatment, it is
very important to employ more precise prophylaxis treatments. In this study
only %14 of participants were from high risk group. Regarding the serious need
for prophylaxis to prevent DVT in this particular population, the expansion of
our studies toward those populations in higher risks is absolutely
suggested.
http://rjms.iums.ac.ir/article-1-2391-en.pdf
Pulmonary Thromboembolism
Deep Vein Thrombosis
Prophylaxis
Prevention.
per
Iran University of Medical Sciences
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article
The Effect of 4-week Swimming Training on Rat’s Knee Osteoarthritis induced by Monosodium Iodoacetate
Seyyed Hossein Mirkarimpour
1
Mohammad Hossein Alizadeh
2
Mohammad Fallah Mohammadi
3
Masoud Adibmoradi
4
Aydin Dilmaghanian
5
University of Tehran
University of Tehran
Guilan University
University of Tehran
University of Tehran
Background: Osteoarthritis is the most common form of
synovial joint diseases which affect old people all around the world and can
lead to chronic pain and severe disability. The aim of present study was to investigate the therapeutic effect of
swimming exercise on MIA-induced osteoarthritis in male rats’ knee joint. Methods: Eighteen Wistar male rats (weight of 173±1 g,
8 weeks old) were randomly divided into 3 experimental groups: intact control,
monosodium Iodoacetate (MIA) only and swimming. Intra-articular injection of
MIA (50µl) was applied on rats’ right knee, similarly saline was injected in
left knees. Training program used in the study was swimming with moderate
intensity for 28 days. After the completion of the protocol, rats were killed
and both knees of the animals were assessed histopathologically. One-way ANOVA
(p<0/05) and post-hoc Tukey test were used for statistical analysis of data. Results: Results indicated that moderate swimming
training for 4 weeks significantly improved osteoarthritic symptoms of rats’
knee in 3 histopathological measures of Depth Ratio of Lesions (p=0/001), Total
Degeneration Width (p=0/001) and Significant Degeneration Width (P=0/001). Conclusion: The findings of the present study showed that
a moderate swimming training program exerts a beneficial influence on cartilage
lesions induced by monosodium iodo acetate injection. Thus, the training
protocol used in this study can be recommended for the treatment of
osteoarthritis.
http://rjms.iums.ac.ir/article-1-2392-en.pdf
Swimming Training
Knee Osteoarthritis
Monosodium Iodoacetate
Rats.