Razi Journal of Medical Sciences
مجله علوم پزشکی رازی
RJMS
Medical Sciences
http://rjms.iums.ac.ir
39
journal39
2228-7043
2228-7051
en
jalali
1400
4
1
gregorian
2021
7
1
28
4
online
1
fulltext
fa
اثر تعامل 4 هفته تمرین استقامتی و اکتاپامین بر بیان ژن PGC-1α در بافت چربی قهوه ای رت های نر تغذیه شده با DFO
Interaction effect of 4 weeks of endurance training and octopamine on gene expression of PGC-1α in brown adipose tissue of male rats fed with DFO
فیزیولوژی ورزش
Exercise Physiology
پژوهشي
Research
<strong><span style="color:#0070c0;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">زمینه و هدف: </span></span></span></strong><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">امروزه، بافت چربی قهوهای به عنوان هدف بالقوه درمانی برای افزایش هزینی انرژی و استفاده از فعالیت ورزشی جهت تحریک این بافت مورد توجه پژوهشگران قرار گرفته است. از این رو، هدف از مطالعهی حاضر، تعیین تأثیر تعامل 4 هفته تمرین استقامتی و اکتاپامین بر بیان ژن </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">PGC-1α</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> در بافت چربی قهوهای رتهای نر تغذیه شده با </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">DFO</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> بود.</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;"></span></span></span></span><br>
<strong><span style="color:#0070c0;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">روش کار:</span></span></span></strong><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> 30 سر رت نر ویستار با میانگین وزن300 تا350 گرم و سن 8 هفته، </span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">بهطور تصادفی به 5 گروه (6=</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">n</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">) </span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">کنترل سالم </span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">، کنترل مسموم (</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">DFO</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">)، تمرین استقامتی + گروه </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">DFO</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> ، اکتاپامین + </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">DFO</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> و تمرین استقامتی + اکتاپامین + </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">DFO</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> تقسیم شدند.</span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> تزریق درون صفاقی </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">ml/kg</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">10 اکتاپامین و گاواژ روغن حرارت دیده، به ترتیب پنج بار در هفته و هر روز انجام شد. پروتکل تمرین استقامتی شامل 4 هفته تمرین هوازی، 5 جلسه در هفته به مدت 20 دقیقه دویدن بر روی تریدمیل بود.</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">بیان ژن</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;"> UCP-1 </span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">توسط </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">RT& PCR</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> اندازه گیری شدند. از آزمون های تی مستقل، تحلیل واریانس دو سویه و آزمون تعقیبی بونفرونی جهت تحلیل دادهها استفاده گردید. </span></span></span><br>
<strong><span style="color:#0070c0;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">یافتهها:</span></span></span></strong><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> نتایج نشان داد، مصرف </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">DFO</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> موجب کاهش معنیدار بیان ژن </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">PGC-1α</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> (05/0</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;"><</span></span></span></span> <span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;"> p</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">) در مقایسه با گروه کنترل سالم شد. تمرین استقامتی باعث افزایش معنیدار بیان ژن </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">PGC-1α</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> (05/0</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;"><</span></span></span></span> <span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;"> p</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">) در مقایسه با گروه </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">DFO</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> شد. اثر تعامل تمرین استقامتی و اکتاپامین باعث افزایش غیر معنیدار بیان ژن </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">PGC-1α</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> (05/0> </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;"> p</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">) در مقایسه با گروه </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">DFO</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> شد.</span></span></span><br>
<strong><span style="color:#0070c0;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">نتیجهگیری:</span></span></span></strong><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> به نظر میرسد، تمرین و اکتاپامین میتوانند از طریق ویژگی آنتیاکسیدانی خود، بیان ژن </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">PGC-1α</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> در بافت چربی قهوهای را فعال کنند.</span></span></span>
<strong>Background & Aims:</strong> Obesity, as a primitive risk factor in type 2 diabetes, is recognized by the imbalance between absorption and energy expenditure. This imbalance is probably due to the combined effect of reduced physical activity and increased supply of fried foods and fast food at reasonable prices. Brown adipose tissue (BAT) has a substantial ability to dissipate excess energy as heat in a process called thermogenesis, which is activated in response to stimulants such as high-fat diets, cold and exercise training. Nowdays, the consumption of fried foods such as french fries has become very popular among human societies. The use of heat causes chemical changes, including oxidation, that can affect mitochondrial function. PGC-1α is the major regulator of brown adipose tissue thermogenesis. Low levels of PGC-1α gene expression increase ROS production and cause oxidative stress. Endurance training is used to prevent and treat obesity, insulin resistance, or type 2 diabetes because of its ability to improve mitochondrial function and fatty acid oxidation. One of the adaptations resulting from exercise is altered expression of the PGC-1α gene. Octopamine is an antioxidant and endogenous antioxidant biogenic amine that has properties similar to catecholamines such as norepinephrine. Octopamine has the ability to stimulate lipolysis and fat metabolism . The present study aimed to determine the interaction effect of 4 weeks of endurance training and octopamine on gene expression of PGC-1α in brown adipose tissue of male rats fed with DFO.<br>
<strong>Methods: </strong>In an experimental study, 30 adult male Wistar rats weighing an average of 300 to 350 g and aged 8 weeks were purchased. All rats were kept in polycarbonate cages (5 mice per cage) at 22°C, 55% humidity and under the light and dark cycle for 12:12 hours without restriction on water and food. Rats were randomly divided into five groups: healthy control (n=6), deep frying oil (DFO, n=6), endurance training + DFO (n=6), octopamine + DFO (n=6) and endurance training + octopamine + DFO (n=6). Intraperitoneal injection of 10 ml/kg of octopamine and gavage of deep -frying oil were done five times a week and every day, respectively.<br>
To adapt the rats in the aerobic training group, before starting the main training program, the rats in this group ran at a speed of 9 m / min for 20 minutes for a week. The endurance training protocol consisted of 4 weeks of endurance training and 5 sessions a week week. The training session included 5 minutes of warm-up at 7 m / min and 5 minutes of cooling at 5 m / min. The intensity of training started in the first week with 50% vo2max and a speed of 16 m / min, and in the last week it reached 65% vo2max and a speed of 26 m / min. To prepare deep frying oil, 8 liters of sunflower oil was heated for 190 consecutive days at a temperature of 190 to 200 ° C for 4 consecutive days.<br>
48 hours after the last training session and 8 hours of fasting, all rats were anesthetized with chloroform and then sacrificed. The brown adipose tissue was immediately removed from the body and stored in a nitrogen tank at -80 ° C. Gene expression of PGC-1α was measured by Real time PCR. Independent t-test, two-way analysis of variance and Bonferoni post hoc tests were used to analyze the data. All the analyses were done by SPSS software version 21 and the charts were drawn using Microsoft Excel software version 16. The significance level was p<0.05.<br>
<strong>Results:</strong> The results showed that consumption of deep frying oil induced significant decrease in gene expression of PGC-1α (p<0.05) compared to the healthy control group. The endurance training caused a significant increase in gene expression of PGC-1α (p>0.05) compared to the DFO group. Effect of octopamine alone and the interaction effect of endurance training and octopamine caused the non-significant increase in PGC-1α gene expression in comparison with the DFO group (P>0.05).<br>
<strong>Conclusion:</strong> The endurance training and octopamine may have influenced PGC-1α gene expression through their antioxidant and lipolytic properties. However, the octopamine group and the interaction group of endurance training and octopamine require changes in the dose, intensity, and duration of endurance training for their effects to be statistically significant. Probably, endurance training has affected PGC-1α gene expression by stimulating elevated levels of catecholamines (epinephrine), stimulation of beta-adrenergic receptors through the upregulation of orexin signals in adipose tissue, and activation of CREB transcription factors. In addition, exercise can increase the gene expression of PGC-1α by increasing and activating NRF2 and binding it to the Antioxidant response element (ARE) and finally the production of antioxidants.
تمرین استقامتی, استرس اکسیداتیو, بافت چربی قهوهای,DFO,PGC-1α
Endurance training, Stress oxidative, Brown adipose tissue, DFO, PGC-1α.
66
74
http://rjms.iums.ac.ir/browse.php?a_code=A-10-5279-2&slc_lang=fa&sid=1
Mohsen
Aminian
محسن
امینیان
m.aminian180@gmail.com
3900319475328460059565
3900319475328460059565
No
Islamic Azad University, Damghan Branch, Semnan, Iran
واحد دامغان، سمنان، ایران
Mohammad Ali
Azarbayjani
محمدعلی
آذربایجانی
azarbayjani@iauctb.ac.ir
3900319475328460059566
3900319475328460059566
Yes
Islamic Azad University, Central Branch, Tehran, Iran
دانشگاه ازاد اسلامی واحد تهران مرکزی
Nemt Allah
Nemti
نعمت الله
نعمتی
Nnemati258@gmail.com
3900319475328460059567
3900319475328460059567
No
Islamic Azad University, Damghan Branch, Semnan, Iran
دانشگاه آزاد اسلامی، واحد دامغان، سمنان، ایران
Tahereh
BagherPoor
طاهره
باقرپور
Bagherpoor_ta@yahoo.com
3900319475328460059568
3900319475328460059568
No
Islamic Azad University, Damghan Branch, Semnan, Iran
دانشگاه آزاد اسلامی، واحد دامغان، سمنان، ایران