Razi Journal of Medical Sciences
مجله علوم پزشکی رازی
RJMS
Medical Sciences
http://rjms.iums.ac.ir
39
journal39
2228-7043
2228-7051
en
jalali
1399
12
1
gregorian
2021
3
1
27
12
online
1
fulltext
fa
اثر تعامل تمرین هوازی با شدت متوسط و اوژنول بر بیان ژن occludin و claudin در بافت قلب رت های مسموم شده با کلرپیریفوس
Interaction effect of moderate intensity aerobic training and eugenol on gene expression of claudin and occludin of cardiac tissue of male rats poisoned with chlorpyrifos
فیزیولوژی ورزش
Exercise Physiology
پژوهشي
Research
<strong><span style="color:#0070c0;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">زمینه و هدف: </span></span></span></strong><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">اتصالات محکم، ساختارهای پویایی هستند که عبور یونها و مولکولها را در بافت قلب تنظیم میکنند. هدف از مطالعهی حاضر، تعیین تأثیر تعامل تمرین</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">هوازی با شدت متوسط و اوژنول بر بیان ژن </span></span></span><em><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">occludin</span></span></span></span></em><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> و </span></span></span><em><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">claudin</span></span></span></span></em><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> در بافت قلب رتهای مسموم شده با کلرپیریفوس بود.</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;"></span></span></span></span><br>
<strong><span style="color:#0070c0;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">روش کار:</span></span></span></strong><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> 40 سر رت نر ویستار با میانگین وزن180 تا220 گرم و سن 8 هفته </span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">بهطور تصادفی به 5 گروه (8= </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">n</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">) </span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">کنترل سالم</span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">، کنترل مسموم (کلرپیریفوس)، تمرین هوازی با شدت متوسط + کلرپیریفوس ، اوژنول + کلرپیریفوس و تمرین هوازی با شدت متوسط + اوژنول + کلرپیریفوس</span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> تقسیم شدند.</span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> تزریق درون صفاقی </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">ml/kg</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">3 کلرپیریفوس و گاواژ </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">mg/kg</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> 250 مکمل اوژنول، به ترتیب به مدت 6 هفته و 4 هفته و هر دو پنج بار در هفته انجام شدند. پروتکل تمرین هوازی شامل 4 هفته تمرین هوازی با شدت متوسط و 5 جلسه در هفته بود.</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">در اولین جلسهی تمرین، سرعت از </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">m/s</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> 11 و مدت زمان 10 دقیقه دویدن بر روی تردمیل شروع شد، به تدریج سرعت و مدت تمرین در طول اجرای پروتکل تمرین افزایش یافت و در جلسهی آخر سرعت به </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">m/s</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> 20 و مدت زمان 26 دقیقه رسید. بیان ژن </span></span></span> <em><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">claudin</span></span></span></span></em><em><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">و</span></span></span></em> <em><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">occludin</span></span></span></span></em> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> توسط </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">RT& PCR</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> اندازه گیری شدند. از آزمونهای تی مستقل، تحلیل واریانس دو سویه و آزمون تعقیبی توکی جهت تحلیل دادهها استفاده گردید. </span></span></span><br>
<strong><span style="color:#0070c0;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">یافتهها:</span></span></span></strong><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> نتایج نشان داد، تمرین هوازی با شدت متوسط باعث افزایش معنی دار بیان ژن </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;"> occludin</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">(001/0= </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">p</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">)</span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> و </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">claudin</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> (001/0= </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">p</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">) در مقایسه با گروه کنترل مسموم شد. اوژنول باعث افزایش معنی دار بیان ژن </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;"> occludin</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">(001/0=</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">p</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">)</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">شد در حالیکه اثرش بر بیان ژن </span></span></span><em><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">occludin</span></span></span></span></em><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> معنی دار نبود (391/0= </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">p</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">). تعامل تمرین هوازی با شدت متوسط و اوژنول باعث افزایش غیر معنی دار بیان ژن </span></span></span><em><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">occludin</span></span></span></span></em> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">(720/0= </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">p</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">) </span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">و </span></span></span><em><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">claudin</span></span></span></span></em><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> (973/0= </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">p</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">). در مقایسه با گروه کنترل مسموم شد.</span></span></span><br>
<strong><span style="color:#0070c0;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">نتیجهگیری:</span></span></span></strong><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> به نظر میرسد، تمرین هوازی با شدت متوسط و اوژنول از طریق ویژگی آنتیاکسیدانی و ضد التهابی خود میتوانند بر بیان ژنهای درگیر در اتصالات محکم بافت قلب موثر باشند.</span></span></span>
<strong>Background & Aims:</strong> Insecticides are a very important group of environmental pollutants and their greatest use is in agriculture to protect against diseases and pests, and to improve the quality of crops (1). The use of pesticides and their potential to enter the human diet has raised many concerns for researchers. Organophosphoruses are toxic compounds that people are constantly exposed to in varying concentrations, and its dangers include cancer. Chlorpyrifos is a type of organophosphorus that is widely used as an insecticide in the world. The results of studies have shown that chlorpyrifos has detrimental effects on various organs including the immune system, reproductive system, liver, biochemical and blood parameters. These studies also showed that this toxin increases oxidative stress in various tissues such as liver and kidneys. The role of oxidative stress in cardiovascular disease, Alzheimer's, diabetes, and damage to tight junction has been confirmed. Tight junction proteins, as one of the important structural components, are essential for regulating the passage of ions and molecules in the heart. Claudin and occludin are the first densely integral membrane proteins in the blood barrier and have similar topography and function interactively with each other. The permeability of the junction and the function of the epithelial barrier are primarily caused by the claudins, and the types of claudin isoforms are expressed there and determine the specific properties of the tissue. Occludin and claudin are both reduced by oxidative stress. As mentioned, the use of agricultural pesticides such as chlorpyrifos causes oxidative stress in the body. Therefore, it seems necessary to use a method that can protect against the effects of oxidative stress and the side effects of this toxin. Eugenol or 4-ally-2-methoxyphenol is an aromatic molecule found in the oils and essential oils of various plants, including clove and cinnamon. Various studies have shown that natural antioxidants with phenolic structure play an important role in protecting body tissues against free radicals. Based on the evidence presented, eugenol has been shown to have an important protective effect against free radical lipid peroxidation (12) and its anti-inflammatory effect has been confirmed. Today, the use of exercise in increasing cardiovascular fitness and physical fitness, improving oxidative stress and vascular tissue has been approved. The aim of this study was to explain the effect of the interaction of moderate-intensity aerobic training and eugenol on the gene expression of occludin and claudin of the cardiac tissue of rats poisoned with chlorpyrifos<br>
<strong>Methods:</strong> 40 male Wistar rats were randomly divided into 5 groups (n = 8): healthy control, poisoned control (chlorpyrifos), moderate intensity aerobic training + chlorpyrifos, eugenol + chlorpyrifos and moderate intensity aerobic training + eugenol + chlorpyrifos. Intraperitoneal injection of 3 ml / kg chlorpyrifos and gavage of 250 ml / kg eugenol supplement were performed five times a week for 6 weeks and 4 weeks, respectively. In order to adapt, rats in the aerobic training group ran for 20 minutes at 9m/min speed for two weeks before starting the main training protocol. The aerobic training protocol consisted of 4 weeks of moderate-intensity aerobic training, 5 sessions per week running on a treadmill. Each training session consisted of 5 minutes of warm-up, 10-26 minutes of moderate-intensity aerobic training (starting at 11 m / s in the first session and reaching 20 m / s in the last session) and 5 minutes of cooling. Gene expression of claudin and occludin were measured by Real time-PCR. Independent t-test, Two-way ANOVA and Tukey post hoc test were used to analysis the data.<br>
<strong>Results:</strong> The results showed that the moderate-intensity aerobic training and eugenol caused significant increase in gene expression of occludin (p=0.001) and claudin (p=0.001) compared to Poisoned control group. eugenol caused significant increase in gene expression of occludin (p=0.001) whereas its effect was not significant on occludin gene expression (p=0.391). Interaction effect of moderate-intensity aerobic training and eugenol caused the non significant increase in claudin (p=0.973) and occludin (p=0.720) gene expression in comparison with Poisoned control group.<br>
<strong>Conclusion:</strong> Chlorpyrifos toxin increases oxidative stress in various tissues of the body and since oxidative stress affects the expression of claudin and occludin, it significantly reduces the expression of their genes in the blood barrier. However, moderate-intensity aerobic training and eugenol supplementation alone, possibly relying on their antioxidant and anti-inflammatory properties by increasing the activity of antioxidants such as SOD and catalase, reducing lipid peroxidation, activating and increasing ERK1 / 2, and MAPK, decreasing TNF-α, and decreasing beta-amyloid induced the increase the expression of claudin and occludin genes. The results of the present study showed that the interaction of moderate-intensity aerobic training and eugenol did not have a significant effect on the expression of claudin and occludin genes, but what is important is the increase compared to the poisoned control group, and physiologically this change is considered a feat, although the precise mechanism of insignificance cannot be clearly explained. In general, it seems that moderate-intensity aerobic training and eugenol can affect tight junctions.<br>
تمرین هوازی با شدت متوسط, استرس اکسیداتیو, claudin, occludin
Moderate intensity aerobic training, Stress oxidative,claudin, occludin
142
151
http://rjms.iums.ac.ir/browse.php?a_code=A-10-5471-1&slc_lang=fa&sid=1
Saba
Almasi
صبا
الماسی
Sabaalmasi8587@gmail.com
3900319475328460056425
3900319475328460056425
No
Islamic Azad University, Central Branch, Tehran, Iran
دانشگاه آزاد اسلامی، واحد تهران مرکزی، تهران، ایران
Mohammad Ali
Azarbayjani
محمدعلی
آذربایجانی
azarbayjani@iauctb.ac.ir
3900319475328460056426
3900319475328460056426
Yes
Islamic Azad University, Central Branch, Tehran, Iran
دانشگاه آزاد اسلامی، واحد تهران مرکزی، تهران، ایران
Maghsoud
peeri
مقصود
پیری
m.peeri@iauctb.ac.ir
3900319475328460056427
3900319475328460056427
No
Islamic Azad University, Central Branch, Tehran, Iran
دانشگاه آزاد اسلامی، واحد تهران مرکزی، تهران، ایران