Razi Journal of Medical Sciences
مجله علوم پزشکی رازی
RJMS
Medical Sciences
http://rjms.iums.ac.ir
39
journal39
2228-7043
2228-7051
en
jalali
1402
2
1
gregorian
2023
5
1
30
3
online
1
fulltext
fa
اثر تعامل تمرین هوازی و اکتاپامین بر فعالیت بافت چربی قهوهای رتهای نر تغذیه شده با روغن عمیق حرارتدیده
Interaction Effect of 4 Weeks Aerobic Training and Octopamine on Activity of Brown Adipose Tissue of Male Rats Fed with Deep Frying Oil
فیزیولوژی ورزش
Exercise Physiology
پژوهشي
Research
<div style="text-align: justify;"><span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="tab-stops:10.5pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:115%"><span style="font-family:Calibri,sans-serif"><b><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:#0070c0">زمینه و هدف:</span></span></span></span></b> <span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">بافت چربی قهوهای از طریق ترموژنز به کاهش وزن بدن کمک مینماید.</span></span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">تمرین ورزشی و مکملهایی مانند اکتاپامین نیز از طریق ویژگی آنتی اکسیدانی و تحریک نوراپی نفرین قادر به فعال کردن فاکتور ویژهی بافت چربی قهوهای هستند. لذا، هدف از مطالعهی حاضر، تبیین تاثیر تعامل تمرین</span></span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">هوازی و اکتاپامین بر فعالیت بافت چربی قهوهای<b> </b>رتهای نر تغذیه شده با روغن عمیق حرارت دیده بود.</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black"></span></span></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="tab-stops:10.5pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:115%"><span style="font-family:Calibri,sans-serif"><b><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:#0070c0">روش کار:</span></span></span></span></b><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black"> 30 سر رت نر ویستار </span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">بطور تصادفی به 5 گروه (6=</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black">n</span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">) </span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">کنترل سالم</span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">، کنترل مسموم، تمرین هوازی + گروه کنترل مسموم، اکتاپامین + کنترل مسموم و تمرین هوازی + اکتاپامین + کنترل مسموم </span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">تقسیم شدند.</span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black"> تزریق درون صفاقی </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black">ml/kg</span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">10 اکتاپامین و گاواژ روغن حرارت دیده، به ترتیب پنج بار در هفته و هر روز انجام شد. پروتکل تمرین هوازی شامل 4 هفته تمرین هوازی، 5 جلسه در هفته به مدت 20 دقیقه دویدن بر روی تریدمیل بود.</span></span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">بیان ژن</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black"> UCP-1 </span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">توسط </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black">RT& PCR</span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black"> اندازه گیری شدند. از آزمونهای تی مستقل، تحلیل واریانس دو سویه و آزمون تعقیبی بونفرونی جهت تحلیل دادهها استفاده گردید. </span></span></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="tab-stops:10.5pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:115%"><span style="font-family:Calibri,sans-serif"><b><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:#0070c0">یافتهها:</span></span></span></span></b><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black"> نتایج نشان داد، تمرین هوازی باعث افزایش معنیدار بیان ژن </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black">UCP-1</span></span></span></span><span style="font-size:10.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black"> (05/0</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black"><</span></span></span></span> <span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black">p</span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">) در مقایسه با گروه کنترل مسموم شد. اثر تعامل تمرین هوازی و اکتاپامین باعث افزایش غیر معنی دار بیان ژن </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black">UCP-1</span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black"> (05/0> </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black">p</span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">) در مقایسه با گروه کنترل مسموم شد.</span></span></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="tab-stops:10.5pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:115%"><span style="font-family:Calibri,sans-serif"><b><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:#0070c0">نتیجهگیری:</span></span></span></span></b><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black"> به نظر میرسد، تمرین و اکتاپامین میتوانند بافت چربی قهوهای را فعال کند.</span></span></span></span></span></span></span></span></span></span></span></span></div>
<div style="text-align: justify;"><span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="tab-stops:10.5pt"><span style="line-height:115%"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:10.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:#0070c0">Background & Aims:</span></span></span></span></b> <span lang="EN" style="font-size:10.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black">Obesity and related diseases such as type 2 diabetes, cardiovascular disease, and various types of cancer are major global health concerns. Increased adipose tissue in the body is one of the causes of obesity. Adipose tissue consists of two categories of white adipose tissue which store energy and brown adipose tissue, which controls the body's thermogenesis. Fatty acids in brown adipose tissue produce heat by burning and maintaining body temperature. Brown adipose tissue contains polyunsaturated lipid stores that provide rapid storage of fatty acids to generate heat and maintain body temperature, and thermogenesis, and has the flexibility to adapt to external stimuli such as cold and sympathetic stimulation for thermogenesis. Brown adipose tissue plays a regulatory role in whole-body metabolism and body weight. Thermogenesis is activated via the β3-adrenoceptor pathway and occurs in mitochondria in brown adipose tissue containing Mitochondrial uncoupling protein-1 (UCP-1). The family of Mitochondrial uncoupling proteins (UCPs) plays an important role in the thermogenesis of brown adipose tissue and the regulation of mitochondrial ROS excretion in various tissues. Changes in lifestyle and diet are one of the achievements of modern life today, which can be attributed to the tendency to overeat fast foods among family members or the community. Consumption of heated oils, in addition to increasing oxidative stress and apoptosis in tissues, leads to obesity by reducing the lipolysis pathway, inhibiting beta-adrenergic receptors, and increasing the accumulation of fat in visceral tissues. Aerobic exercise is used to prevent and treat obesity, insulin resistance, or type 2 diabetes because of its ability to improve mitochondrial function and fatty acid oxidation, reduce body fat, improve insulin sensitivity, and absorb glucose into skeletal muscle. Octopamine is an antioxidant and endogenous antioxidant biogenic amine that has properties similar to catecholamines such as norepinephrine. Octopamine has the ability to stimulate lipolysis and fat metabolism. The aim of the present study was to determine the interaction effect of 4 weeks of aerobic training and octopamine on the activity of brown adipose tissue of male rats fed with deep frying oil.</span></span></span></span><span dir="RTL" lang="AR-SA" style="font-size:10.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black"></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="tab-stops:10.5pt"><span style="line-height:115%"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:10.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:#0070c0">Methods:</span></span></span></span></b><span style="font-size:10.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black"> In an experimental study, 30 adults male Wistar rats weighing an average of 300 to 350 g and aged 8 weeks were purchased. All rats were kept in polycarbonate cages (5 mice per cage) at 22 2 2 ° C, 55% humidity, and under the light and dark cycle for 12:12 hours without restriction on water and food. Rats were randomly divided into five groups: healthy control (n=6), DFO (n=6), aerobic training + DFO (n=6), octopamine + DFO (n=6), and aerobic training + octopamine + DFO (n=6). Intraperitoneal injections of 10 ml/kg of octopamine and Gavage of deep frying oil were done five times a week and every day, respectively.</span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="tab-stops:10.5pt"><span style="line-height:115%"><span style="font-family:Calibri,sans-serif"><span style="font-size:10.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black">In order to adapt the rats in the aerobic training group, before starting the main training program, the rats in this group ran at a speed of 9 m / min for 20 minutes for a week. The aerobic exercise protocol consisted of 4 weeks of aerobic exercise and 5 sessions per week. The training session included 5 minutes of warm-up at 7 m / min and 5 minutes of cooling at 5 m / min. The intensity of training started in the first week with 50% vo2max and a speed of 16 m / min, and in the last week, it reached 65% vo2max and a speed of 26 m / min. To prepare deep frying oil, 8 liters of sunflower oil was heated for 190 consecutive days at a temperature of 190 to 200 ° C for 4 consecutive days.</span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="tab-stops:10.5pt"><span style="line-height:115%"><span style="font-family:Calibri,sans-serif"><span style="font-size:10.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black">48 hours after the last training session and 8 hours of fasting, all rats were anesthetized with chloroform and then sacrificed. The brown adipose tissue was immediately removed from the body and stored in a nitrogen tank at -80 ° C. Gene expression of UCP-1 was measured by Real-time PCR. Independent t-test, two-way analysis of variance, and Bonferroni post hoc tests were used to analyze the data. All the analyses were done by SPSS software version 21 and the charts were drawn using Microsoft Excel software version 16. The significance level was p <0.05. </span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="tab-stops:10.5pt"><span style="line-height:115%"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:10.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:#0070c0">Results:</span></span></span></span></b> <span style="font-size:10.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black">The results showed that consumption of deep frying oil induced a significant decrease in gene expression of UCP-1 (P < 0.05) compared to the healthy control group. The aerobic training and octopamine caused a significant increase in gene expression of UCP-1 (P >0.05) compared to the DFO group. The interaction effect of aerobic training and octopamine caused a nonsignificant increase in UCP-1 gene expression (P >0.05) in comparison with the DFO group.</span></span></span></span><span dir="RTL" lang="AR-SA" style="font-size:10.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black"></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="tab-stops:10.5pt"><span style="line-height:115%"><span style="font-family:Calibri,sans-serif"><b><span lang="EN" style="font-size:10.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:#0070c0">Conclusion:</span></span></span></span></b> <span lang="EN" style="font-size:10.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black">Decreased expression of UCP-1 following consumption of deep heated oil may be due to disruption of metabolic pathways that have reduced metabolism. Regarding the significant increase in UCP-1 gene expression under the influence of aerobic exercise and octopamine supplementation, each of them alone, we can first mention the antioxidant properties of aerobic exercise and octopamine. According to previous studies, the variable UCP-1 itself is an antioxidant. And since aerobic exercise and octopamine, each has antioxidant properties on their own, they can enhance and increase the expression of the UCP-1 gene in brown adipose tissue. Increasing UCP-1 can reduce the excess ROS produced and act as a compatible mechanism to reduce the harmful effects of ROS. It is possible that exercise was able to stimulate brown adipose tissue through sympathetic enhancement, which in turn increased UCP-1 gene expression and mitochondrial biogenesis. Another reason is that octopamine is similar to norepinephrine. Octopamine can bind rapidly to β3 adrenergic receptors, increase cAMP gene expression, and increase lipolysis and fat metabolism in general. It seems that exercise and octopamine can activate brown adipose tissue.</span></span></span></span></span></span></span></span></span></span></div>
تمرین هوازی, استرس اکسیداتیو, بافت چربی قهوهای فعال, UCP-1
Aerobic training, Stress oxidative, Brown adipose tissue, Deep frying oil.
244
253
http://rjms.iums.ac.ir/browse.php?a_code=A-10-5279-1&slc_lang=fa&sid=1
Mohsen
Aminian
محسن
امینیان
3900319475328460075585
3900319475328460075585
No
Department of Exercise Physiology, Islamic Azad University, Central Branch, Tehran, Iran
گروه فیزیولوژی ورزشی، دانشگاه آزاد اسلامی، واحد دامغان، سمنان، ایران
Mohammad Ali
Azarbayjani
محمدعلی
آذربایجانی
m_azarbayjani@iauctb.ac.ir
3900319475328460075586
3900319475328460075586
Yes
Professor, Department of Exercise Physiology, Islamic Azad University, Central Branch, Tehran, Iran
استاد، گروه فیزیولوژی ورزشی، دانشگاه آزاد اسلامی، واحد تهران مرکزی، تهران، ایران
Nemt Allah
Nemti
نعمت الله
نعمتی
3900319475328460075587
3900319475328460075587
No
Associate Professor, Department of Exercise Physiology, Islamic Azad University, Damghan Branch, Semnan, Iran
دانشیار، گروه فیزیولوژی ورزشی، دانشگاه آزاد اسلامی، واحد دامغان، سمنان، ایران
Tahereh
BagherPoor
طاهره
باقرپور
3900319475328460075588
3900319475328460075588
No
Assistant professor, Department of Exercise Physiology, Islamic Azad University, Damghan Branch, Semnan, Iran
استادیار، گروه فیزیولوژی ورزشی، دانشگاه آزاد اسلامی، واحد دامغان، سمنان، ایران