Razi Journal of Medical Sciences
مجله علوم پزشکی رازی
RJMS
Medical Sciences
http://rjms.iums.ac.ir
39
journal39
2228-7043
2228-7051
en
jalali
1400
1
1
gregorian
2021
4
1
28
2
online
1
fulltext
fa
اثر تمرین هوازی و مکمل ویتامین D بر آپوپتوز بافت کبد رتهای قرار گرفته در معرض آب اکسیژنه
The effect of aerobic exercise and vitamin D supplementation on hepatic Apoptosis levels in rats exposed to oxygenated water
فیزیولوژی ورزش
Exercise Physiology
پژوهشي
Research
<strong><span style="color:#0070c0;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">زمینه و هدف:</span></span></span></strong> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">به­نظر می­رسد متابولیت­های فعال ویتامین </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">D</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> و فعالیت ورزشی نقش مهمی در کاهش آسیب­های</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">اکسایشی</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">ایفا می­کنند.</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">هدف از انجام مطالعه حاضر، بررسی اثر تمرین هوازی و مکمل ویتامین</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">D</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> بر آپوپتوز بافت کبد موش های قرار گرفته در معرض آب اکسیژنه (</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">H<sub>2</sub>O<sub>2</sub></span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> ) بود. </span></span></span><br>
<strong><span style="color:#0070c0;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">روش کار:</span></span></span></strong> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">در این مطالعه تجربی، تعداد 60 سرموش نر نژاد ویستار با میانگین وزن 20</span></span></span><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">±</span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">220 گرم به طور تصادفی به 10 گروه تقسیم شدند. حیوانات به مدت 8 هفته تحت مداخلات القا استرس اکسیداتیو (تزریق درون صفاقی </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">H<sub>2</sub>O<sub>2</sub></span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> به صورت 3 بار در هفته)، مصرف ویتامین </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">D</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> (به صورت تزریق روزانه درون صفاقی) و ورزش بر روی تردمیل (30 الی 60 دقیقه، با سرعت 8 الی 20 متر/دقیقه) قرار گرفتند. 24 ساعت پس از آخرین جلسه تمرینیموش ها قربانی شده و بافت کبد استخراج گردید. میزان پروتئین کاسپاز-8 با استفاده از روش الایزا اندازه­گیری شد. جهت مقایسه بین گروهی از آزمون آماری تحلیل واریانس یک طرفه استفاده شد. </span></span></span><br>
<strong><span style="color:#0070c0;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">یافته­ها: </span></span></span></strong><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">نتایج نشان داد که میزان پروتئین کاسپاز-8 در گروه­های </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">H</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">، </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">2H</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">،</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">HD </span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> و </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">2HD</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> در مقایسه با گروه کنترل بالاتر بود (05/0></span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">p</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">). همچنین، گروه­های </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">HE</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> و </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">HDE</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> در مقایسه با گروه </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">H</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> و گروه­های </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">2HE</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> و </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">2HDE</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> در مقایسه با گروه </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">2H</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> میزان کمتری از پروتئین کاسپاز-8 را در بافت کبد نشان دادند (05/0></span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">p</span></span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">). </span></span></span><br>
<strong><span dir="RTL"><span style="color:#0070c0;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">نتیجه­گیری:</span></span></span></span></strong> <span dir="RTL"><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;">نتایج نشان داد که قرار دادن</span></span></span></span><span dir="RTL"><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> موش ها در معرض آب اکسیژنه موجب القای آپوپتوز در بافت کبد می­شود. ورزش منظم توانست تا حدود زیادی این اثر را تعدیل نماید اما مصرف مکمل ویتامین </span></span></span></span><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">D3</span></span></span><span dir="RTL"><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:10.0pt;"> چنین اثری به دنبال نداشت</span></span></span></span>
<strong>Background and Aims:</strong> Most chronic diseases are associated with an imbalance between the formation of reactive oxygen species (ROS) and antioxidant enzymes, which leads to deviation from the steady state. This process is called oxidative stress. One of the complications of oxidative stress is liver dysfunction. In this case, programmed cell death (apoptosis) appears to increase in liver cells and damage the tissue. Increased production of ROS without adequate antioxidant defense leads to oxidative stress and cell death and apoptosis, which is associated with various diseases and abnormalities. However, some studies have shown that exercise is associated with reduction in apoptosis. In this regard, Fernandez et al. (2012) showed that 10 weeks of regular swimming training in male rats increased anti-apoptotic proteins (Bcl-2 and Bcl-x) and decreased apoptotic proteins (BAD). also, Chen et al showed that 11 weeks of treadmill running increased Bcl-2 in rats with doxorubicin-induced chronic kidney disease, while caspase-8 levels decreased. On the other hand, studies have shown the benefits of vitamin D on liver health. Recent studies have reported an association between vitamin D3 deficiency and the incidence of liver disease. According to the research’s mentioned and in response to the ambiguities caused by the apoptotic or protective effects of exercise in various physiological situations, including exposure to oxidative stress, which has an undeniable effect on the body and vital organs, including the liver. Therefore, the present study intends to investigate the question of whether regular aerobic exercise and vitamin D supplementation effect on hepatic Apoptosis levels in rats exposed to oxygenated water?<br>
<strong>Methods:</strong> This is an experimental study. This research was conducted in 2019 on Wistar rats in Kerman University of Medical Sciences, Kerman, Iran. 60 male Wistar rats weighed 220±20 g was randomly divided into ten groups. Then, The animals were exposed to oxidative stress (intraperitoneal injection of H<sub>2</sub>O<sub>2</sub> 3 times a week), vitamin D3 (intraperitoneal injection daily) and exercise on treadmill for 8 weeks (30 to 60 min, at speeds of 8 to 20 m/min) after the last training session liver tissue was extracted and Caspase-8 protein concentration measured by ELISA method.<br>
<strong>Results:</strong> The results showed that Caspase-8 protein levels were higher in the H, 2H, HD and 2HD groups compared to the control group (p<0.01).Also, HE and HDE groups as well as 2HE and 2HDE groups showed lower levels of Caspase-8 protein in liver tissue compared to H and 2H groups, respectively (p<0.001).<br>
<strong>Conclusion:</strong> Vitamin D can enhance the production of anti-inflammatory mediators and appears to regulate immune function. Zheng et al. (2017) reported that vitamin D receptor deficiency in human breast and prostate cancers significantly induces cell apoptosis by regulating the Wnt / β-catenin signaling pathway. The reasons for this discrepancy in the results can be traced to the dose of vitamin D consumed, which was higher in the studies than in the present study.<br>
The findings of this study were consistent with the results of Fernandes et al. (2012) and Chen et al. Increased potential activity of antioxidant enzymes (not measured in the present study) may be practiced as one of the mechanisms for controlling apoptosis in animals. It is recommended that regular aerobic exercise be considered to reduce hepatic apoptosis. In the present study, there were limitations in the present study, including the study of animal specimens. Other limitations of this study include lack of measurement of other apoptosis-related factors.<br>
the results showed that exposure of rats to H<sub>2</sub>O<sub>2</sub> induces apoptosis in liver tissue. regular treadmill exercise was able to attenuate this effect to a large extent, but vitamin D3 supplementation did not.
آپوپتوز؛ کاسپاز-8؛ کبد؛تمرین هوازی؛ویتامین D
Apoptosis, Caspase-8, Liver, Vitamin D, Aerobic exercise
23
32
http://rjms.iums.ac.ir/browse.php?a_code=A-10-5076-2&slc_lang=fa&sid=1
Hamid Reza
Etemadi
حمیدرضا
اعتمادی
etemadihr@gmail.com
3900319475328460056855
3900319475328460056855
No
Islamic Azad University, South Tehran Branch, Tehran, Iran
دانشکده تربیت بدنی و علوم ورزشی، تهران، ایران
Sajad
Arshadi
سجاد
ارشدی
Arshadi.sajad@yahoo.com
3900319475328460056856
3900319475328460056856
Yes
Islamic Azad University, South Tehran Branch, Tehran, Iran
دانشکده تربیت بدنی و علوم ورزشی، گروه فیزیولوژی ورزش، تهران، ایران
Abdolali
Banaeifar
عبدالعلی
بنایی فر
alibanaeifar@yahoo.com
3900319475328460056857
3900319475328460056857
No
Islamic Azad University
دانشکده تربیت بدنی و علوم ورزشی، تهران، ایران
Mohammadali
azarbayjani
محمدعلی
آذربایجانی
m_azarbayjani@iauctb.ac.ir
3900319475328460056858
3900319475328460056858
No
Islamic Azad University
دانشکده تربیت بدنی و علوم ورزشی, گروه فیزیولوژی ورزش، تهران، ایران