TY - JOUR JF - RJMS JO - RJMS VL - 24 IS - 163 PY - 2018 Y1 - 2018/1/01 TI - Effect of Iron oxide nanoparticles and magnetic field on neurogenesis and Nestin gene expression after ischemic reperfusion in rat TT - اثر نانوذرات اکسید آهن و میدان مغناطیسی در عصب زایی و بیان ژن Nestin بعد از ایسکمی ریپرفیوژن در رت N2 - Background: Ischemic stroke is a common cause of adult disability and death worldwide that leads to damage in neuronal networks and neurovascular units and ceasation of brain functions. In this study the effect of iron oxide nanoparticles and magnetic field on neurogenesis after ischemic reperfusion (IR) in rat model was evaluated. Methods: In this experimental study 50 male Wistar rats weighing 220-250 g were randomly divided into five groups of 10 rats each: including control, sham (ischemic reperfusion model), IR + iron oxide nanoparticles (10mg/kg), IR+magnetic field (1 Tesla, 20 min in 4 days), and IR + iron oxide nanoparticles and magnetic field groups. Injections were performed intraperitoneally. Neurogenesis was evaluated in hippocampus of 5 groups after 4 days by H&E staining method. Nestin gene expression was quantitatively investigated in 5 groups by Real-time PCR. Results: In this study it was revealed that the iron oxide nanoparticles as well as the magnetic field increase the neurogenesis rate after ischemic reperfusion during 4 days (p<0.05). The expression of Nestin gene increased in iron oxide nanoparticles treated group and the magnetic field exposed group significantly (p<0.05) as compared to ischemic reperfusion model. However, their combined treatment did not show a significant difference compared with the sham group within 4 days. Conclusion: Our result suggests iron oxide nanoparticles as well as magnetic field separately can be two effective methods in ischemia therapy. SP - 70 EP - 79 AU - bagheri, Ayshin AU - Khazaei Koohpar, Zeinab AU - Falahati, Mojtaba AD - Islamic Azad University, Tonekabon, Iran. KW - iron oxide nanoparticles KW - ischemic reperfusion KW - magnetic field KW - Nestin KW - Real-time PCR UR - http://rjms.iums.ac.ir/article-1-4881-en.html ER -