RT - Journal Article T1 - Investigation Angiogenesis Effect of Silver Nanoparticles Coated with Silymarin onChick Embryo Chorioalantoic Membrane and Evaluation of Its Antioxidant Activity JF - RJMS YR - 2017 JO - RJMS VO - 23 IS - 152 UR - http://rjms.iums.ac.ir/article-1-4303-en.html SP - 54 EP - 64 K1 - Angiogenesis K1 - Silver nanoparticles K1 - Chorioallantoic membrane K1 - Chicken K1 - Antioxidant AB - Background: Angiogenesis is a complex, active phenomenon, which plays a significant role in physiologic and pathologic processes. Silymarins are combination of flavonoids that have antioxidant and anti-inflammatory effects. In the current research, the effects of silver nanoparticles coated with silymarin on the angiogenesis of the chorioallantoic membrane of chicken fetal and its antioxidant property have been explored. Methods: Ross Fertilized Chicken eggs were distributed randomly and equally in control group, laboratory control group, and four experimental groups with different densities. In the second day of incubation, some windows were formed on the eggs and, on the eighth day, they were treated. On the twelfth day, some photographs were taken from the subjects’ chorioallantoic membranes, the number and length of the vessels were measured, using the Image J software. Also the Drabkyn test was executed to determine the level of hemoglobin and the obtained results were analyzed as significant (P<0.05), using SPSS software and T-Test. Further, to evaluate the antioxidant activities of silver nanoparticles coated with silymarin, DPPH and ABTS tests were used. Results: The comparison between the average of the number and length of the vessels in control group and the experimental groups 2, 3, and 4 showed significant reduction. The comparison between the fetal crown-rump length and weight in control group and the experimental groups 3 and 4 showed significant reduction. Also, the average amount of hemoglobin density in the experimental group 4 showed significant reduction, in comparison to the control group. According results from antioxidant assay, the nanoparticles showed dose dependent radical inhibited activity. Conclusion: In addition, the nanoparticles lead to dose dependent reduction in angiogenesis and increase the removal of free radicals. LA eng UL http://rjms.iums.ac.ir/article-1-4303-en.html M3 ER -