TY - JOUR JF - RJMS JO - RJMS VL - 8 IS - 25 PY - 2001 Y1 - 2001/12/01 TI - ADENYLYL CYCLASE ASSAY TO MEASURE MACROPHAGE CHEMOTACTIC PROTEIN-1 RECEPTOR FUNCTION TT - اندازه‌گیری فعالیت آدنیلیل سیکلاز غشاء سلولی در حضور پروتئین کموتاکسیک ماکروفاژ N2 -  Adenylyl cyclase is a membrane-bound enzyme that catalyzes the conversion of ATP to cAMP. The inhibition of adenylyl cyclase was carried out by measuring the ability of the macrophage chemotactic protein-1 to inhibit the forskolin-induced enzyme activity. Measurement of adenylyl cyclase activity was performed according to the procedure described by Wiegn.  Adenylyl cyclase activity in the present macrophage chemotactic protein-1 was decreased compared to that in controls [2.11+/- 0.15(mean +/-SD.)vs. 6.83+/-0.45, activity ( µ mol cAMP/mg protein/min)].  Macrophage chemotactic protein significant reduced adenylyl cyclase activity by 79.9%. SP - 283 EP - 287 AU - Qujeq, D. AU - Ahmadi, M. AD - KW - 1) Adenylyl cyclase 2) Macrophage chemotactic protein-1 UR - http://rjms.iums.ac.ir/article-1-348-en.html ER -