RT - Journal Article T1 - The effect of omega-3 supplementation and high-fat diet in brain-derived neurotrophic factor (BDNF) in the male adult rat hippocampus JF - RJMS YR - 2014 JO - RJMS VO - 21 IS - 119 UR - http://rjms.iums.ac.ir/article-1-3101-en.html SP - 42 EP - 48 K1 - Brain-derived neurotrophic factor (BDNF) K1 - Omega-3 Supplementation K1 - High-fat diet K1 - Hippocampus AB - Background: This study was to examine the effects of omega-3 supplementation and high-fat diet in brain-derived neurotrophic factor (BDNF) in the male adult rat hippocampus. Methods: Twenty one Wistar rats were divided into three groups: (1) regular diet (RD), (2) high-fat (HF) diet and (3) Docosahexaenoic acid (DHA) diet. RD group was regarded as control. Animals in HF groups were exposed to the HF diet for 8 weeks and animals in DHA groups were treated with 100/mg/kg/day of DHA via oral gavage for 8 weeks. Hippocampal BDNF protein was assessed using commercial ELISA kits and the data was analyzed by one-way ANOVA. When appropriate, further differences were analyzed by LSD post hoc test. Statistical differences were considered significant at p<0.05. Results: The results showed that the HF diet had no significant effect in BDNF protein level (p=0.873), while the DHA diet revealed a significant increase in BDNF protein level comparison with the RD (p=0.008) and HF (p=0.006) group Conclusion: According to results of the present study, DHA diet can increase the amount of hippocampus BDNF protein level. LA eng UL http://rjms.iums.ac.ir/article-1-3101-en.html M3 ER -