Volume 25, Issue 9 (12-2018)                   RJMS 2018, 25(9): 26-36 | Back to browse issues page

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akbari M, shahidi F, Rajabi H, kashef M, mazaheri Z. The simultaneous effect of six weeks forced swimming and crocin supplementation on the expression of 3-cardiomyocyte genesase 3 in male rats infected with hydrogen peroxide. RJMS. 2018; 25 (9) :26-36
URL: http://rjms.iums.ac.ir/article-1-5371-en.html
Shahid Rajaee Teacher Training University , akbariteach@gmail.com
Abstract:   (240 Views)
Introduction: There is a clear evidence to support the importance of regular physical activity and the use of antioxidants in the prevention or control of cardiovascular diseases, especially those that cause cardiac apoptosis. Our aim in this study was to determine the simultaneous effect of six weeks forced swimming and crocin supplementation on the expression of 3 cardiomyocyte genesase in male rats infected with hydrogen peroxide.
Methodology: 36 male Wistar rats with an average weight of 180-200 g were randomly divided into six groups of sham injections (saline), hydrogen peroxide, hydrogen peroxide and crocin, hydrogen peroxide and forced swimming, hydrogen peroxide and forced swimming and crocin, control group. The forced swimming protocol was held in two stages in training and exercises, the first phase of training in the first week, the forced swimming was extended every 10 minutes to 60 minutes, then the training period was 60 minutes and 5 days a week for 6 The week was running. For induction of H2O2 at 1 mg / kg / kg body weight for 3 weeks and one day in the form of sub peritoneal injection and 30 minutes before the injections. Crocin was injected with purity of 98% and dose of 12.5 mg / kg daily with 5 cc of distilled water in the peritoneum. Rats were sacrificed by CO2 gas 48 hours after the last session of activity and the specimens were immediately transferred to liquid nitrogen, and RNA was extracted manually by Sigma Solution Trisol solution. Then, for the synthesis of cDNA, the RNA extracted by the cDNA synthesis kits was performed using kit formantase. Finally, ABI Step One Real Time PCR was used to express the Caspase 3 gene. To confirm the incidence of apoptosis, tissue samples were assessed by Tannel method. Statistical analysis was performed using Kolmogorov-Smirnov test for natural distribution. Then, the variance (Levin test) and Perth data were analyzed. Finally, one-way ANOVA was used to test the statistical hypotheses and the Scheffe post hoc test was used.
Results: hydrogen peroxide induction was associated with a significant increase in expression of Caspase 3 gene expression and six weeks of forced swimming resulted in a significant decrease in Caspase 3 gene expression in rats' myocardial tissue, as well as interaction of six weeks of forced swimming and induction of Crocin, Caspase 3, which is completely visible in the results of the Tunnel.
Conclusion: Regular forced swimming as well as crocin consumption separately led to a significant reduction in gene expression. Caspase 3 was found in the rat's myocardium tissue. On the other hand, the combination of training and crocin had a synergistic effect on inhibiting Caspase 3 gene expression in hydrogen Peroxide poisoned rats.
Type of Study: Research | Subject: Exercise Physiology

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