Volume 23, Number 147 (9-2016)                   RJMS 2016, 23(147): 74-81 | Back to browse issues page


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alijani H, Matroodi S, sharafi A, zamani I. Antimicrobial activity of marine actinomycete Nocardiopsis sp. AHA2 synthesized silver nanoparticles against microbial pathogens. RJMS. 2016; 23 (147) :74-81
URL: http://rjms.iums.ac.ir/article-1-3979-en.html

assistant professor 1- Department of Marine biology, Faculty of Marine Science, Khoramshahr University of marine science and technology, Iran.
Abstract:   (1605 Views)

Background: Silver nanoparticles have been widely used in various biomedical applications and drug delivery systems. The biosynthesis of nanoparticles has been proposed as a cost effective environmental friendly alternative to chemical and physical methods. In the present study, synthesis of nanoparticles using a novel Nocardiopsis sp. AHA2 has been attempted. We used culture supernatant of Nocardiopsis sp. AHA2 for the biologically synthesis of silver nanoparticles and study of its antimicrobial activity. Methods: marine Sediment samples were collected from depth of 10th centimeter. Serial dilution was mad for screening of marine actinomycetes using SCA medium. After Isolation and characterization of Nocardiopsis sp. AHA2, The reduction of silver ions occurred when silver nitrate solution (0.01 M) was treated with the Nocardiopsis sp. AHA2 culture supernatant at room temperature. nanoparticles were characterized by UV-visible, Zeta potential. Antimicrobial activity of the prepared silver nanoparticles against bacteria and fungi pathogens was assayed using well diffusion assay. Results: Silver nanoparticles were successfully synthesized in the culture supernatant of Nocardiopsis sp. AHA2. During the experiment, the appearance of a dark brown color indicated the formation of silver nanoparticles. In the UV-visible spectrum, maximum peak was observed between 420 nm, indicating the presence of AgNPs. AgNPs displayed antimicrobial activity against all used pathogenic microorganisms. Nocardiopsis sp. AHA2 can be a good candidate for the synthesis of the AgNPs using silver nitrate.

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Type of Study: Research | Subject: Biology

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