Volume 8, Issue 25 (12-2001)                   RJMS 2001, 8(25): 283-287 | Back to browse issues page

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Qujeq D, Ahmadi M. ADENYLYL CYCLASE ASSAY TO MEASURE MACROPHAGE CHEMOTACTIC PROTEIN-1 RECEPTOR FUNCTION. RJMS. 2001; 8 (25) :283-287
URL: http://rjms.iums.ac.ir/article-1-348-en.html
Abstract:   (4805 Views)

 Adenylyl cyclase is a membrane-bound enzyme that catalyzes the conversion of ATP to cAMP. The inhibition of adenylyl cyclase was carried out by measuring the ability of the macrophage chemotactic protein-1 to inhibit the forskolin-induced enzyme activity. Measurement of adenylyl cyclase activity was performed according to the procedure described by Wiegn.

  Adenylyl cyclase activity in the present macrophage chemotactic protein-1 was decreased compared to that in controls [2.11+/- 0.15(mean +/-SD.)vs. 6.83+/-0.45, activity ( µ mol cAMP/mg protein/min)].

  Macrophage chemotactic protein significant reduced adenylyl cyclase activity by 79.9%.

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