Volume 18, Number 89 (11-2011)                   RJMS 2011, 18(89): 10-19 | Back to browse issues page


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Barzegari Firouzabadi F, Javed A, Rezaei Zarchi S. Study of Follicle Stimulating Hormone (FSH) Efficiency on the In vitro Growth of Mice Follicles in Presence of Animal Different Sera. RJMS. 2011; 18 (89) :10-19
URL: http://rjms.iums.ac.ir/article-1-1956-en.html

MSc in Animal Physiology
Abstract:   (3622 Views)

Background

ovarian follicle growth and oocyte maturation. In vitro follicular culture systems, at various

developmental stages, allow the identification of these factors and the understanding of their

mechanisms of action. Keeping in mind the significance of the influence of environmental factors on

the follicle growth, this work focuses on the effect of some endocrine and paracrine factors on the

growth and differentiation of preantral follicles during the in vitro follicular culture using the rodent

(mouse) model.

: Several endocrine and locally acting factors are involved in the complex process of

Methods

serum type. Prepubertal gilt serum, embryonic stem cell tested fetal calf serum, hypogonadal mouse

serum and fetal calf serum were tested during the present study. Effect of the culture period was also

evaluated on the follicle growth. After carefully selecting the optimum growth conditions, the effect

of FSH was evaluated on the growth and viability of the follicular and oocyte maturation. Different

concentrations of FSH (5, 20, 40, 60, 100, 140, 180 and 220 mIU/l) were added to the culture medium

(containing 25-30 follicles each) during separate experiments.

: Our study was semi-experimental. First task of the study was to choose an appropriate

Results

During the present experiment, 100 mIU/ml FSH showed highly significant effect on the follicle and

oocyte growth. Follicle survival rate also increased (91%) as compared to that grown without this

gonadotropin (28%). Oocyte maturation (61%) and germinal vesicle breakdown rates (81%) also

showed an increase (p

: After experiments, fetal calf serum (FCS) was chosen for the evaluation of the effect of FSH.0.05).

Conclusion

antrum had a positive effect on the follicle survival and oocyte robustness.

: These results have suggested that the exposure to FSH and FCS before the formation of
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Type of Study: Research | Subject: Physiology

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