Volume 15, Number 59 (summer 2008)                   RJMS 2008, 15(59): 157-164 | Back to browse issues page


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Moeini Alishah, F, Hosseini Gohari, L, Shamsian, B, Mosta'an, L. The Separation of Globin Chains by Ion Exchange Chromatography inorder to Identify Hemoglobinopathies. RJMS. 2008; 15 (59) :157-164
URL: http://rjms.iums.ac.ir/article-1-1020-en.html

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Abstract:   (5366 Views)

Background & Aim:

Since the mutant Hbs do not have any obvious electrical charge, globin chain separation is helpful for the diagnosis of

unknown Hbs. Therefore, the present study was carried out to detect alpha or beta chain variants by cation exchange

chromatography.

There are several point mutations in hemoglobin(Hb) genes which can cause hemoglobinopathy.

Material and Method:

count(CBC), HbA2 and HbF percentages were determined by routine methods, and cellulose acetate and citrate agar

electrophoresis were performed on all the samples. For HbS confirmation, solubility test was performed and globin chains

were separated by carboxymethyl cellulose(CMC) chromatography in the presence of 8 M urea. SPSS version 14 was used

for statistical analysis.

In this descriptive study, 20 samples having abnormal Hb were selected. Complete blood cell

Results:

agar electrophoresis. CMC chromatography revealed that three patients had abnormal beta chains, 15 had abnormal alpha

chains, and 2 remaining samples had abnormal alpha and beta chains.

According to the obtained results, all of the samples had an abnormal band on cellulose acetate and citrate

Conclusion:

helpful guideline for the selection of an appropriate gene for DNA sequencing. Moreover, this method is useful to screen the

cases for hemoglobinopathies and beta thalassemia coexistance, which can sometimes lead to severe clinical

manifestations.

Mutant alpha and beta chains can be detected by CMC chromatography and globin chain separation is a
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Type of Study: Research | Subject: Biochemistry

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