Volume 24, Issue 157 (7-2017)                   RJMS 2017, 24(157): 1-7 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

ahmadi R, saberi B, Noroozi S, rahimi S, Moulavi P. The effects of Rhamnus frangula miller leaf extract on breast cancer cells viability in cell culture. RJMS 2017; 24 (157) :1-7
URL: http://rjms.iums.ac.ir/article-1-4459-en.html
Islamic Azad University of North Tehran , pooriamoulavi@yahoo.com
Abstract:   (6215 Views)

Background: Various studies have shown that plant extracts may have cytotoxic effects on cancer cells. The aim of this study was to determine the effects of Rhamnus frangula miller leaf on viability of breast cancer cells in cell culture.
Methods: In this laboratory experimental study breast cancer cell line (MCF-7) was used. MCF cells were cultured in RPMI 1640 culture medium containing 10% fetal bovine serum and 100 unit/ml penicillin and 100 µg/ml streptomycin and were kept in incubator at 37 C and at pressure of 5% CO2. The cells were randomly divided into control group and groups exposed to 10, 1, 0.1, 0.01 and 0.001 mg/ml of Rhamnus frangula leaf extract. Viability of cells was assayed using MTT test. The data were analyzed using ANOVA.
Results: There was no significant difference between viability of MCF7 cells in group exposed to 0.001 mg/ml of extract and control group. However, viability of MCF7 cells significantly decreased in groups exposed to 0.01, 0.1, 1 and 10 mg/ml of extract compared with control group (p<0.05).
Conclusion: Our findings indicated that low dose of the Rhamnus frangula leaf extract has no significant cytotoxic effect on MCF-7 cells. However, high doses of extract of have cytotoxic effects on MCF-7 cells.
 

Full-Text [PDF 231 kb]   (2204 Downloads)    
Type of Study: Research | Subject: Biology

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | Razi Journal of Medical Sciences

Designed & Developed by : Yektaweb